Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin

Abreu, Marcia Matos de; Pereira, Luiz Henrique Garcia; Vila, Vilma Barretto; Foresti, Fausto; Oliveira, Cláudio

doi:10.1590/s1415-47572009005000075

Cited by 21 publications

(22 citation statements)

References 40 publications

(34 reference statements)

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“…Expected heterozigosity was very high in local populations, ranging from 0.553 to 0.839. A high global value of f (0.254; P < 0.05) was noted, with values within local populations ranging from 0 to 0.6, indicating nonrandom mating and low effective population sizes (similar to those found by de Abreu et al, 2009 andPereira et al, 2009 Table 2. Microsatellite panels (1 and 2), markers (locus), forward primer sequence, fluorescent dye and annealing temperature prime (TA) used in the study.…”

Section: Resultssupporting

confidence: 69%

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Telles¹,

Collevatti²,

Braga³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular genetic variation (estimated using 7 microsatellite markers) of Pseudoplatystoma punctifer, by using samples obtained from 15 localities along the Madeira River and Solimões, Amazon Basin. A high genetic diversity was observed associated with a relatively low F ST (0.057; P < 0.001), but pairwise F ST values ranged from zero up to 0.21 when some pairs of populations were compared. These F ST values Geographical genetics of Pseudoplatystoma punctifer have a relatively low correlation with geographic distances (r = 0.343; P = 0.074 by Mantel test), but a Mantel correlogram revealed that close populations (up to 80 km) tended to be more similar than expected by chance (r = 0.360; P = 0.015). The correlogram also showed a exponential-like decrease of genetic similarity with distance, with a patch-size of around 200 km, compatible with isolation-by-distance and analogous processes related to local constraints of dispersal and spatially structured levels of gene flow. The pattern revealed herein has important implications for establishing strategies to maintain genetic diversity in the species, especially considering the threats due to human impacts caused by building large dams in this river system.

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Section: Resultssupporting

confidence: 69%

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Telles¹,

Collevatti²,

Braga³

et al. 2014

Genet. Mol. Res.

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“…Before characterizing the genetic variability of pure populations; however, it important identify if the fishes are pure species or hybrids [17][18][19]. There have been studies of the genetic variability of P. corruscans and P. reticulatum using microsatellite molecular markers [12,20,21], but none have genetically identified the individuals in order to determine whether they were genetically pure before performing the study of genetic variability. This lack of genetic identification has occurred not only with P. corruscans and P. reticulatum, but also in studies of the "tambaqui" Colossoma macropomum [22,23] and the "pacu" Piaractus mesopotamicus [24][25][26][27], which also have commercially produced hybrids.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of pure Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum individuals in the Paraná and Paraguay River basins

et al. 2016

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The presence of interspecific hybrid surubim (Pseudoplatystoma corruscans × Pseudoplatystoma reticulatum) in natural environments can put at risk the existence of the pure parental lineages. The aim of this study was to characterize the genetic variability of pure Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum individuals from rivers in the Paraná and Paraguay basins in Brazil. Seventy-six pure individuals of P. corruscans and 16 of P. reticulatum were evaluated with six microsatellite loci for both species, along with one species-specific locus each for P. corruscans and P. reticulatum. Loss of heterozygosity was confirmed, and preservation measures for P. corruscans and P. reticulatum are needed in order not to lose further genetic variation. In addition, we confirmed that these markers are useful for the management of pure stocks in natural environments, for fish breeding, and in Biology Electronic supplementary material The online version of this article (

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“…Microsatellites are abundantly distributed along genomes and demonstrate high levels of allelic polymorphism (DeWoody & Avise, 2000). The molecular structure and genetic variability of microsatellites are extensively exploited in evolutionary studies of a wide variety of fish species (see Chistiakov et al, 2006), including some from Neotropical freshwater (Hrbek et al, 2007;Abreu et al, 2009;Calcagnotto & DeSalle, 2009;Matsumoto & Hilsdorf, 2009;Pereira et al, 2009;Sanches et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus

Melo

Sato²,

Foresti

et al. 2013

Neotrop. ichthyol.

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The rio São Francisco basin contains many endemic species, such as Prochilodus argenteus and P. costatus, which have great commercial importance. However, information about the main recruitment sites and genetic studies containing extensive sampling of these species are scarce. To investigate the roles of the marginal lagoons in the maintenance of genetic variability and in the population structure, we analyzed six microsatellite loci in nine sampling groups of P. argenteus and five sampling groups of P. costatus. Our results showed high levels of genetic variability and low values of genetic differentiation for P. argenteus (F ST = 0.008, P < 0.05) and for P. costatus (F ST = 0.031, P < 0.05). In addition, high values of gene flow combined with a small genetic distance suggest the presence of a single population for each species in the middle rio São Francisco basin. Moreover, putative migration routes involving marginal lagoons during the reproductive season could be detected, confirming the importance of these nurseries in the lifecycle of these species. Our results also indicate the necessity of adequate management of the fish resources and the conservation of the floodplains in the rio São Francisco basin.A bacia do rio São Francisco contém muitas espécies endêmicas, tais como Prochilodus argenteus e P. costatus, os quais têm grande importância comercial. Entretanto, informações sobre as principais áreas de recrutamento e estudos genéticos contendo uma extensa amostragem dessas espécies no rio São Francisco são escassas. Para investigar o papel das lagoas marginais na manutenção da variabilidade genética e na estruturação populacional dessas espécies, nós analisamos seis loci microssatélites em nove grupos amostrais de P. argenteus e cinco grupos amostrais de P. costatus. Nossos resultados revelaram altos níveis de variabilidade genética para ambas as espécies e valores baixos de diferenciação genética para P. argenteus (F ST = 0.008, P < 0.05) e P. costatus (F ST = 0.031, P < 0.05). Adicionalmente, valores altos de fluxo gênico combinados com a distância genética baixa sugerem a presença de uma única população para cada espécie no médio rio São Francisco. Possíveis rotas migratórias envolvendo lagoas marginais durante o período reprodutivo puderam ser detectadas, confirmando a importância das lagoas marginais no ciclo de vida dessas espécies. Nossos resultados também indicaram a necessidade de um manejo adequado dos recursos pesqueiros e a conservação das várzeas na bacia do rio São Francisco.

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Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin

Cited by 21 publications

References 40 publications

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Genetic variability of pure Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum individuals in the Paraná and Paraguay River basins

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus

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