Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141

Deng, Wenqian; Sun, Huaqin; Tao, Dachang; Zhang, Sizhong; Ma, Yongxin

doi:10.1590/s1415-47572009005000062

Cited by 5 publications

(2 citation statements)

References 25 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, RNF183 exerts a positive effect on the proliferation and metastasis of CRC cells by activating NF-κB-IL-8 axis [22]. Importantly, the previous studies find that RNF141 promotes spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization in mouse spermatogenesis [23], and exerts a broad function on the development of embryos [24]. Another study shows that there is an increased expression of RNF141 in Barrett's esophagus or esophageal adenocarcinoma than that in normal esophagus [16].…”

Section: Introductionmentioning

confidence: 93%

RNF141 interacts with KRAS to promote colorectal cancer progression

et al. 2021

View full text Add to dashboard Cite

RING finger proteins (RNFs) play a critical role in cancer initiation and progression. RNF141 is a member of RNFs family; however, its clinical significance, roles, and mechanism in colorectal cancer (CRC) remain poorly understood. Here, we examined the expression of RNF141 in 64 pairs of CRC and adjacent normal tissues by real-time PCR, Western blot, and immunohistochemical analysis. We found that there was more expression of RNF141 in CRC tissue compared with its adjacent normal tissue and high RNF141 expression associated with T stage. In vivo and in vitro functional experiments were conducted and revealed the oncogenic role of RNF141 in CRC. RNF141 knockdown suppressed proliferation, arrested the cell cycle in the G1 phase, inhibited migration, invasion and HUVEC tube formation but promoted apoptosis, whereas RNF141 overexpression exerted the opposite effects in CRC cells. The subcutaneous xenograft models showed that RNF141 knockdown reduced tumor growth, but its overexpression promoted tumor growth. Mechanistically, liquid chromatography-tandem mass spectrometry indicated RNF141 interacted with KRAS, which was confirmed by Co-immunoprecipitation, Immunofluorescence assay. Further analysis with bimolecular fluorescence complementation (BiFC) and Glutathione-S-transferase (GST) pull-down assays showed that RNF141 could directly bind to KRAS. Importantly, the upregulation of RNF141 increased GTP-bound KRAS, but its knockdown resulted in a reduction accordingly. Next, we demonstrated that RNF141 induced KRAS activation via increasing its enrichment on the plasma membrane not altering total KRAS expression, which was facilitated by the interaction with LYPLA1. Moreover, KRAS silencing partially abolished the effect of RNF141 on cell proliferation and apoptosis. In addition, our findings presented that RNF141 functioned as an oncogene by upregulating KRAS activity in a manner of promoting KRAS enrichment on the plasma membrane in CRC.

show abstract

Section: Introductionmentioning

confidence: 93%

RNF141 interacts with KRAS to promote colorectal cancer progression

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The amphioxus zfp665 contains four sequential C2H2 zinc finger DNA binding domains at its N-terminus ( Fig 8B), and we were unable to identify a clear orthologue in zebrafish or other species that we have searched. Nevertheless, several zinc finger domain-containing proteins have been found to regulate axial patterning in Drosophila [59], zebrafish [60] and Xenopus [61]. We hypothesize that the overexpressed amphioxus zfp665 may behave like some unidentified endogenous C2H2 zinc finger protein to control zebrafish eve1 expression.…”

Section: Plos Geneticsmentioning

confidence: 94%

Molecular asymmetry in the cephalochordate embryo revealed by single-blastomere transcriptome profiling

Lin

et al. 2020

PLoS Genet

View full text Add to dashboard Cite

Studies in various animals have shown that asymmetrically localized maternal transcripts play important roles in axial patterning and cell fate specification in early embryos. However, comprehensive analyses of the maternal transcriptomes with spatial information are scarce and limited to a handful of model organisms. In cephalochordates (amphioxus), an early branching chordate group, maternal transcripts of germline determinants form a compact granule that is inherited by a single blastomere during cleavage stages. Further blastomere separation experiments suggest that other transcripts associated with the granule are likely responsible for organizing the posterior structure in amphioxus; however, the identities of these determinants remain unknown. In this study, we used high-throughput RNA sequencing of separated blastomeres to examine asymmetrically localized transcripts in two-cell and eight-cell stage embryos of the amphioxus Branchiostoma floridae. We identified 111 and 391 differentially enriched transcripts at the 2-cell stage and the 8-cell stage, respectively, and used in situ hybridization to validate the spatial distribution patterns for a subset of these transcripts. The identified transcripts could be categorized into two major groups: (1) vegetal tier/germ granule-enriched and (2) animal tier/anterior-enriched transcripts. Using zebrafish as a surrogate model system, we showed that overexpression of one animal tier/anterior-localized amphioxus transcript, zfp665, causes a dorsalization/anteriorization phenotype in zebrafish embryos by downregulating the expression of the ventral gene, eve1, suggesting a potential function of zfp665 in early axial patterning. Our results provide a global transcriptomic blueprint for early-stage amphioxus embryos. This dataset represents a rich platform to guide future characterization of molecular players in early amphioxus development and to elucidate conservation and divergence of developmental programs during chordate evolution.

show abstract