Karyotype characterization and nucleolar organizer regions of marsupial species (Didelphidae) from areas of Cerrado and Atlantic Forest in Brazil

Abstract

The karyotypes of 23 specimens belonging to 16 species from nine genera of Brazilian marsupials (family Didelphidae) were studied. The animals were collected in eight localities of Cerrado or Atlantic Forest biomes in the states of Goiás, Tocantins and São Paulo. The karyotypes were analyzed after conventional Giemsa staining and silver staining of the nucleolus organizer regions (Ag-NORs). New karyotypic data were obtained for Gracilinanus microtarsus . Although the karyotypes were relatively conserved with respect to the morphology of the autosomes among species with the same diploid number, some differences regarding FN, sex chromosomes morphology and Ag-NORs patterns were detected.

Comparative cytogenetics of some marsupial species (Didelphimorphia, Didelphidae) from the Amazon basin

“… Geographic locations of Caluromys philander individuals and its sexual chromosomes morphology data in South America. Literature data represented by empty circles and present work represented by full circles: (● 14) Venezuela, Reig et al 1977 ; (○ 4) Purus River; (○ 6) Manaus city, urban área: Federal University of Amazonas’s campus (UFAM); (● 15) Manaus REMAN (Isaac Saba Oil Refinery), present work and Souza et al 2013 ; (○ 10) Trombetas River; (○ 11) Tapajós River; (○ 12) Jari River, Souza et al 2013 ; (● 16) Pernambuco State, Souza et al 1990 ; (● 17) São Paulo State, Svartman and Vianna-Morgante 1999 and Pereira et al 2008 . m = metacentric; sm = submetacentric; a = acrocentric; d = dot-like.…”

“… Geographic locations of Marmosa murina individuals and its X chromosome morphology data in South America. Literature data represented by empty circles and present work represented by full circles: (● 18) Villa Vivêncio, Colômbia, Hayman and Martin 1974 ; (● 20) Loreto-Peru, Reig et al 1977 ; (● 19) Bolívar, Venezuela, Reig et al 1977 ; (○ 3) Negro River; (○ 2) Juruá River; (○ 4) Purus River; (○ 8) Uatumã River; (○ 10) Trombetas River; (○ 12) Jari River; (● 16) Pernambuco State, Souza et al 1990 ; (● 21) Tartarugalzinho, Amapá State State, Carvalho et al 2002 ; (● 22) Vila Rica Mato Grosso State, Pagnozzi et al 2002 ; (● 23) UHE Peixe Angical,TO, Pereira et al 2008 ; (● 24) Tocantins State, Lima 2004 ; (● 25) Uruaçú, Goiás State , Carvalho et al 2002 ; (● 26) Colinas do Sul, Goiás state; (● 27) UHE Corumbá IV Luziania, Goiás state Pereira et al 2008 ; (● 28) Pacoti, Ceará State, Pagnozzi et al 2002 ; (● 29) Espírito Santo State, Paresque et al 2004 . m = metacentric; sm = submetacentric; a = acrocentric.…”

“…4 , locality 14) and areas in the western, central and eastern Amazon (Fig. 4 , localities 4, 6, 12 and 15) ( Reig et al 1977 , Svartman and Vianna-Morgante 1999 , Pereira et al 2008 ). Interestingly, both homozygote and heterozygote females were recorded in central Amazonia near Manaus (Fig.…”

“…01) (not shown here), we verified that multiple NORs are distributed in two distinct lineages: the first in species of the genus Marmosa and the second in species with 22 chromosomes of the genera Didelphis and Philander Brisson, 1762. The mapping of the simple condition onto the phylogenetic tree depicts a wide distribution for this character, present at the base of the tree ( Caluromys philander , C. lanatus , Glironia venusta ) and in at least one or more species of the remaining major clades ( Gracilinanus emiliae , Marmosops spp., Metachirus nudicaudatus , Monodelphis touan , Monodelphis kunsi, and Monodelphis dimidiata ) ( Souza et al 1990 , Palma and Yates 1996 , Carvalho et al 2002 , Svartman and Vianna-Morgante 2003 , Pereira et al 2008 , Souza et al 2013 ). This distribution of NOR character on the didelphid phylogeny is thus congruent with the hypothesis advanced by Hsu et al (1975) that the single NOR is an ancestral state.…”

Citotaxonomia e evolução cromossômica em Oligoryzomys (Rodentia, Sigmodontinae).

“…Akodon montensis (FAGUNDES, 1997), Chaetomys subspinosus (VILELA et al, 2009), Sciurus aestuans ingrami (FAGUNDES et al, 2003) e Trinomys iheringi (YONENAGA-YASSUDA et al, 1985) e de algumas espécies de marsupiais sulamericanos (PEREIRA et al, 2008;SVARTMAN, 2009).…”