Molecular variability in the maize grey leaf spot pathogens in Brazil

Brunelli, Kátia Regiane; Dunkle, Larry D.; Sobrinho, C. Athayde; Fazza, Ana Carolina; Camargo, L. E. A.

doi:10.1590/s1415-47572008005000010

Cited by 12 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A larger number (79) of C. zeae-maydis isolates revealed a similar high genetic similarity (93%) from the same AFLP study. The same AFLP primers were used to score 104 loci in 41 C. zeae-maydis isolates and 28 C. zeina isolates from different regions of Brazil, and similar high levels (>92%) of genetic similarity within species was observed (Brunelli et al 2008). In contrast, Dunkle and collegues screened 80 C. zeae-maydis isolates from a single site in Illinois using 10 microsatellite markers and reported high genetic diversity (79 haplotypes; within-marker gene diversity of 0.61-0.91) (Molecular Ecology Resources Primer Development Consortium, 2009).…”

Section: Zeina (Molecular Ecology Resources Primer Development Conmentioning

confidence: 96%

Cercospora zeina from Maize in South Africa Exhibits High Genetic Diversity and Lack of Regional Population Differentiation

Müller

Barnes

Kunene³

et al. 2016

Phytopathology®

View full text Add to dashboard Cite

South Africa is one of the leading maize-producing countries in sub-Saharan Africa. Since the 1980s, Cercospora zeina, a causal agent of gray leaf spot (GLS) of maize, has become endemic in South Africa, and is responsible for substantial yield reductions. To assess genetic diversity and population structure of C. zeina in South Africa, 369 isolates were collected from commercial maize farms in three provinces (KwaZulu-Natal, Mpumalanga and North West). These isolates were evaluated with fourteen microsatellite markers and species-specific mating type markers that were designed from draft genome sequences of C. zeina isolates from Africa (CMW 25467) and USA (USPA-4). Sixty alleles were identified across 14 loci, and gene diversity values within each

show abstract

Section: Zeina (Molecular Ecology Resources Primer Development Conmentioning

confidence: 96%

Cercospora zeina from Maize in South Africa Exhibits High Genetic Diversity and Lack of Regional Population Differentiation

Müller

Barnes

Kunene³

et al. 2016

Phytopathology®

View full text Add to dashboard Cite

show abstract

“…Carson et al (2002) and Brunelli et al (2008) previously observed that some isolates from the former group II, now C. zeina, did not produce Cercosporin.…”

Section: Introductionmentioning

confidence: 94%

Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. maydis

Koshikumo

Alves

Pinho

2014

Agraria

View full text Add to dashboard Cite

Cercosporin is a phytotoxin with toxic activity against large number of organism, it is produced by hypha in the culture medium. This way, the objective of this study was to induce the production of Cercosporin in Cercospora zeina (CZ) and Cercospora sorghi f. sp. maydis (CS) in 16 culture media, and to verify which medium is most suitable for detection of the toxin. Agar plugs were extracted and they were put in KOH for 16 hours. Cercosporin concentration was determined by using a molar extinction coefficient of 23300 L mol -1 . Under the conditions in which the fungi were cultured, it was not possible to induce the toxin in CZ, which reinforces the hypothesis that the toxin is only produced in planta. CS produced a high concentration of toxin in coconut water Sococo ® medium with yeast extract, 177.34 nmol plug -1. Only the M1D medium did not produce the toxin. There was a difference in the production of toxin depending on the brand and concentration of PDA medium. It is concluded that the media with coconut water Sococo ® are efficient in the induction of Cercosporin in vitro by CS, which are promising for research in this area. Furthermore, CZ did not produce the toxin in the studied conditions. Key words: Cercosporin, maize gray leaf spot, Zea mays L. Indução e detecção de toxina em Cercospora zeinae Cercospora sorghi f. sp. maydis RESUMO A cercosporina é uma fitoxina com atividade tóxica contra um amplo espectro de organismos, produzida pelas hifas em meio de cultura. O objetivo deste estudo foi induzir a toxina em Cercospora zeina (CZ) e Cercospora sorghi f. sp. maydis (CS) em 16 meios de cultura e averiguar qual o meio mais adequado para se detectar a toxina. Discos miceliais foram imersos em KOH por 16 horas e a concentração de toxina foi calculada utilizando-se o coeficiente de 23.300 L mol -1 . Nas condições em que foram submetidos os fungos não foi possível induzir a toxina em CZ, fato que reforça a hipótese de que a toxina é produzida apenas em planta. Em CS, a produção de toxina foi intensa nos meios com água de coco da marca Sococo ® , principalmente quando foi adicionado extrato de leveduras, apresentando 177.34 nmol disco -1 enquanto o meio M1D não foi capaz de induzir a toxina. A diferença na produção de toxina em BDA é devida à concentração e às marcas comerciais diferentes. Conclui-se que os meios com água de coco Sococo ® são eficientes na indução da Cercosporina in vitro por CS, o que constitui uma substância promissora para estudos dessa linha de pesquisa; além disto, CZ não produziu a toxina nas condições estudadas neste trabalho. Palavras-chave:Cercosporiose do milho, Zea mays L., Cercosporina

show abstract

“…Gray leaf spot (GLS) is one of the most significant yieldlimiting foliar diseases of maize (Zea mays L.) in Brazil (Brunelli et al 2008) and several other countries (Ward et al 1999). Until recently, GLS was considered to be primarily caused by C. zeae-maydis Tehon & E. Y. Daniels, with C. sorghi var.…”

Section: Introductionmentioning

confidence: 99%

“…Worldwide, C. zeae-maydis was reported in the USA, Canada, Mexico, China, and Brazil, whereas C. zeina is reported in the eastern United States, Sub-Saharan African countries, Brazil and China (Wang et al 1998;Goodwin et al 2001;Zhu et al 2002;Okori et al 2003;Shim and Dunkle 2005;Crous et al 2006;Brunelli et al 2008;Meisel et al 2009;Groenewald et al 2012;Liu and Xu 2013).…”

Section: Introductionmentioning

confidence: 99%

Cercospora zeina is the main species causing gray leaf spot in southern and central Brazilian maize regions

Neves

Silva

Pereira

et al. 2015

Trop. plant pathol.

View full text Add to dashboard Cite

Maize (Zea mays) fields from a tropical central (Goiás state) and a subtropical south region (Paraná state) of Brazil were surveyed for Cercospora species causing gray leaf spot (GLS), a foliar fungal disease of increasing concern to growers of the country. Single spore isolates were obtained from maize leaves showing GLS symptoms and species-specific primers were used for species identification. In addition, a phylogeny of isolates representative of each species was conducted based on partial DNA sequencing of the EF1-α, CAL, and HIS genes, and ITS-5.8S rDNA region. In the regional collections, Cercospora zeina was the dominant species in Goiás (68/75 isolates) and Paraná (73/75 isolates), followed by C. zeae-maydis and C. sorghi var. maydis, respectively. In three field collections of both states, C. zeina was the sole species found. The phylogenetic characterization of isolates representing C. zeina (n=18), C. zeae-maydis (n=6), and C. sorghi var. maydis (n=2) confirmed the identity of the species and grouped them in well-supported clades.

show abstract

Molecular variability in the maize grey leaf spot pathogens in Brazil

Cited by 12 publications

References 18 publications

Cercospora zeina from Maize in South Africa Exhibits High Genetic Diversity and Lack of Regional Population Differentiation

Cercospora zeina from Maize in South Africa Exhibits High Genetic Diversity and Lack of Regional Population Differentiation

Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. maydis

Cercospora zeina is the main species causing gray leaf spot in southern and central Brazilian maize regions

Contact Info

Product

Resources

About