Leptin and hypothalamic gene expression in early- and late-maturing Bos indicus Nellore heifers

Vaiciunas, Aline; Coutinho, Luiz Lehmann; Meirelles, Flávio Vieira; Pires, Alexandre Vaz; Silva, Luís Felipe Prada e

doi:10.1590/s1415-47572008000400010

Cited by 12 publications

(10 citation statements)

References 41 publications

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“…However, the authors reported that early-maturing heifers had reduced expression of two hypothalamic NPY receptors, NPY1R and NPY4R. The results of the present study, together with the results of Vaiciunas et al (2008), support the hypothesis that signaling through the NPY receptors modulates both nutrition and genetics effects on the attainment of puberty in Nellore heifers.…”

Section: Discussionsupporting

confidence: 82%

“…As observed in this study, the reduction of NPY1R expression by high energy intake suggests that it would make the hypothalamus less sensitive to NPY inhibition, enabling earlier onset of puberty. The role of NPY and its receptors in modulating puberty attainment of early-and late-maturing heifers was analyzed by Vaiciunas et al (2008), who reported that NPY expression was not significantly altered in early-maturing heifers. However, the authors reported that early-maturing heifers had reduced expression of two hypothalamic NPY receptors, NPY1R and NPY4R.…”

Section: Discussionmentioning

confidence: 99%

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Effect of high energy intake on carcass composition and hypothalamic gene expression in Bos indicus heifers

et al. 2017

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-The objective of this study was to evaluate the effect of high or low energy intake on carcass composition and expression of hypothalamic genes related to the onset of puberty. Twenty-four prepubertal Nellore heifers, 18-20-months-old, with 275.3±18.0 kg body weight (BW), and 4.9±0.2 (1-9 scale) body condition score (BCS) were randomly assigned to two treatments: high-energy diet (HE) and low-energy diet (LE). Heifers were housed in two collective pens and fed diets formulated to promote average daily gain of 0.4 (LE) or 1.2 kg (HE) BW/day. Eight heifers from each treatment were slaughtered after the first corpus luteum detection -considered as age of puberty. The 9-10-11th rib section was taken and prepared for carcass composition analyses. Samples from hypothalamus were collected, frozen in liquid nitrogen, and stored at −80 °C. Specific primers for targets (NPY, NPY1R, NPY4R, SOCS3, OXT, ARRB1, and IGFPB2) and control (RPL19 and RN18S1) genes were designed for real-time PCR and then the relative quantification of target gene expression was performed. High-energy diets increased body condition score, cold carcass weight, and Longissimus lumborum muscle area and decreased age at slaughter. High-energy diets decreased the expression of NPY1R and ARRB1 at 4.4-fold and 1.5-fold, respectively. In conclusion, the hastening of puberty with high energy intake is related with greater body fatness and lesser hypothalamic expression of NPY1 receptor and of β-arrestin1, suggesting a less sensitive hypothalamus to the negative effects of NPY signaling.Key Words: cattle, energy supplementation, neuropeptides, puberty, reproduction Revista Brasileira de Zootecnia

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Effect of high energy intake on carcass composition and hypothalamic gene expression in Bos indicus heifers

et al. 2017

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“…The late onset to puberty, which is generally observed in B. indicus when compared with B. taurus, directly affects profit in the cattle industry since it can postpone the start of the reproductive life of a cow for one year. Hence, the development of tools for the identification of more efficient cattle in this regard is of great commercial interest (Vaiciunas et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genome wide association study on early puberty in Bos indicus

Nascimento¹,

Matos

Seno

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to evaluate a genome wide association study (GWAS) approach to identify single nucleotide polymorphisms (SNPs) associated with fertility traits (early puberty) in Nellore cattle (Bos indicus). Fifty-five Nellore cows were selected from a herd monitored for early puberty onset (positive pregnancy at 18 months of age). Extremes of this phenotype were selected; 30 and 25 individuals were pregnant and non-pregnant, respectively, at that age. DNA samples were genotyped using a high-density SNP chip (>777.000 SNP). GWAS using a case-control strategy highlighted a number of significant markers based on their proximity with the Bonferroni correction line. Results indicated that chromosomes 5, 6, 9, 10, and 22 were associated with the traits of interest. The most significant SNPs on these chromosomes were rs133039577, rs110013280, rs134702839, rs109551605, and rs41639155. Candidate genes, as well as quantitative trait loci (QTL) previously reported in the Ensembl and Cattle QTLdb databases, were further investigated. Analysis of the regions close to the SNP on chromosomes 9 and 10 revealed that four QTL had been previously classified under the reproduction category. In conclusion, we have identified SNPs in close proximity to genes associated with reproductive traits. Moreover, U6 spliceosomal RNA was present on three different chromosomes, which is possibly associated with age at first calving, suggesting that it might be a strong candidate for future studies.

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“…A similar effect was reported by Vaiciunas et al (2008), which verified that precocious heifers had lower expression of hypothalamic NPY receptors.…”

Section: Introductionsupporting

confidence: 86%

Estudo do mecanismo molecular da progesterona e do estradiol sobre o início da puberdade em novilhas Nelore

Magalhães¹

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AGRADECIMENTOSAgradeço a Deus e a Nossa Senhora por me fazerem forte, me manterem firme, segura dos meus objetivos, e persistente. Agradeço toda a proteção e às bênçãos recebidas.À minha família, meu maior tesouro, meu chão, meu mundo. Em especial à minha mãe, Leila, meu porto seguro e meu maior apoio, a quem tanto amo. Ao meu pai que me apresentou outro mundo e a quem tenho aprendido a compreender. Aos meus avós, Maria e Afonso, fontes de inspiração, alicerces da nossa família, exemplos de união e amores da minha vida. À minha avó Estelita, muitas saudades e ao avô Ernesto que não tive oportunidade de conhecer. Aos meus tios e padrinhos, Keila (amiga inseparável e verdadeira), Marciano (incentivador das minhas idéias mais malucas), tio Júnior (pai dos meus amores), Marlon, Sônia, Netinho, Smênia, Valmira, Xandy, Néia, Vanusa, Alípio, Toninho, Mara, eternos cúmplices, amigos, apoios, pessoas que ajudaram a formar minha personalidade e me ajudaram a ser uma pessoa melhor, obrigada por confiarem em mim. Aos meus irmãos Newton, Daniel, Grégory, parceiros eternos de vida, farras, bons momentos e dificuldades e às minhas irmãs Isabela e Lívia, minhas lindas! Amo a todos vocês! Aos meus primos lindos, Neto, Gu, Bia, Arthur, Lorrane, Igor, Fred, Breno, Grazian, Dani (e família), amo vocês demais apesar da distância. Aos meus dois afilhados Arthurzinho e Gu, que me desmontam a cada passagem por Brasília. E o agradecimento mais especial aos novos presentes de Deus que trouxeram vida nova e alegria à família meus sobrinhos Esther, Enzo e Anna.A todos os meus amigos de Brasília, que entendem o motivo da minha distância, mas não menor consideração.À FAPESP pela concessão da bolsa de doutorado e pelo auxílio financeiro à pesquisa, que me possibilitaram ter tranquilidade no desenvolvimento de minhas atividades. À Prefeitura do Campus da USP de Pirassununga por disponibilizar as instalações do abatedouro escola e parte dos animais.Ao professor Luis Felipe Prada e Silva, por todo o apoio, confiança, amizade e estrutura, sem os quais não seria possível o desenvolvimento do trabalho. E por todo o aprendizado e vivência fora do projeto de pesquisa, mas dentro do contexto do LPGC, que só me auxiliam a crescer e me fazem uma melhor profissional a cada dia. The understanding of the molecular mechanisms by which nutrition, genetics and hormonal treatments affect the beginning of puberty is of great importance for developing strategies aiming to reduce the age at first calving, and therefore increase the slaughter rate in Nellore cattle. The effects of progesterone device and of endogenous estradiol on the molecular mechanisms controlling the attainment of puberty in Nellore heifers were investigated.Specifically, the molecular pathways of progesterone and estradiol were studied in the hypothalamus. Thirty five non-pubertal heifers, between 13 and 14 months of age, were divided into four treatment (nine or eight per treatment): P4 device without estradiol (SP), P4 device with estradiol (PE), without P4 device and without estradiol (SS...

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Leptin and hypothalamic gene expression in early- and late-maturing Bos indicus Nellore heifers

Cited by 12 publications

References 41 publications

Effect of high energy intake on carcass composition and hypothalamic gene expression in Bos indicus heifers

Effect of high energy intake on carcass composition and hypothalamic gene expression in Bos indicus heifers

Genome wide association study on early puberty in Bos indicus

Estudo do mecanismo molecular da progesterona e do estradiol sobre o início da puberdade em novilhas Nelore

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