5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers

Alves-Costa, Fernanda Antunes; Martins, Cesar; Matos, Fernanda Del Campos de; Foresti, Fausto; Oliveira, Cláudio; Wasko, Adriane Pinto

doi:10.1590/s1415-47572008000200025

Cited by 6 publications

(6 citation statements)

References 27 publications

(20 reference statements)

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“…To date, the 5S rDNA has scarcely been studied in the Sciaenidae family, and in only one species ( Isopisthus parvipinnis ) two variants of 5S rDNA have been obtained (Alves‐Costa et al . ). Neither of the two NTSs presented homology with the NTS of A. regius , making this region suitable for the designing of species‐specific markers.…”

Section: Resultsmentioning

confidence: 97%

“…If the whole Sciaenidae family is considered in respect of genetic data, there are only two studies on the cytogenetics of Brazilian species and on 5S rDNA characterization in certain species respectively (Accioly & Molina ; Alves‐Costa et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…The Sciaenidae fish family comprises 282 known species distributed into 70 genera, found in the Indian, Pacific and Atlantic Oceans (Accioly & Molina ). Most species of this family are important fishery resources (Alves‐Costa, Martins, Del Campos de Matos, Foresti, Oliveira & Wasko ). The range of Argyrosomus regius Asso, known as the meagre, extends along the coast from Norway to the Congo, including the Mediterranean and Black Seas.…”

Section: Introductionmentioning

confidence: 99%

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First approach to studying the genetics of the meagre (Argyrosomus regius; Asso, 1801) using three multigene families

et al. 2012

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The meagre (Argyrosomus regius) is a commercially important species for fisheries, and aquaculture production has been increasing in recent years. However, this growing importance has not been matched by increased genetic knowledge of the species. For this reason, an initial approach has been made to understanding the genetics of the meagre, using three particular multigene families (45S and 5S ribosomal DNA and U2 snRNA gene) as both molecular and cytogenetic markers. Only one type of 5S rDNA and only one ITS-1 (from 45S rDNA) were obtained, and these therefore could provide the basis for the development of genetic markers for this species. However, the U2 snRNA gene produced a multi-band electrophoretic pattern, and some of these bands were demonstrated to be linked with the U1 snRNA gene. This linkage could also provide a good species-specific marker which could be useful for tracing the evolutionary history of the Sciaenidae family. In this study, we have described for the first time the karyotype of the meagre: this is composed of 48 acrocentric chromosomes. Each of the three gene families were localized on different chromosome pairs, although the U2 snRNA gene probe was also located scattered throughout the genome of the meagre, as expected by the amplification pattern.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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First approach to studying the genetics of the meagre (Argyrosomus regius; Asso, 1801) using three multigene families

et al. 2012

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“…In most eukaryotes, these sequences are organized in tandem repetitions, in which the (NTS)non transcribed spacers present high interspecific variations, due to insertions/deletions, minisatellites and pseudogenes (Nelson and Honda 1985, Leah et al 1990, Alves-Costa et al 2008). However, although evolutionarily conserved, stochastic events may promote a great dispersal of the 5S rDNA sequences in a large number of chromosomes in some Perciformes species (Affonso and Galetti 2005, Lima-Filho et al 2014b).…”

Section: Discussionmentioning

confidence: 99%

Co-located 18S/5S rDNA arrays: an ancient and unusual chromosomal trait in Julidini species (Labridae, Perciformes)

Amorim¹,

Cioffi²,

Bertollo³

et al. 2016

CCG

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Wrasses (Labridae) are extremely diversified marine fishes, whose species exhibit complex interactions with the reef environment. They are widely distributed in the Indian, Pacific and Atlantic oceans. Their species have displayed a number of karyotypic divergent processes, including chromosomal regions with complex structural organization. Current cytogenetic information for this family is phylogenetically and geographically limited and mainly based on conventional cytogenetic techniques. Here, the distribution patterns of heterochromatin, GC-specific chromosome regions and Ag-NORs, and the organization of 18S and 5S rDNA sites of the Atlantic species Thalassoma noronhanum (Boulenger, 1890), Halichoeres poeyi (Steindachner, 1867), Halichoeres radiatus (Linnaeus, 1758), Halichoeres brasiliensis (Bloch, 1791) and Halichoeres penrosei Starks, 1913, belonging to the tribe Julidini were analyzed. All the species exhibited 2n=48 chromosomes with variation in the number of chromosome arms among genera. Thalassoma noronhanum has 2m+46a, while species of the genus Halichoeres Rüppell, 1835 share karyotypes with 48 acrocentric chromosomes. The Halichoeres species exhibit differences in the heterochromatin distribution patterns and in the number and distribution of 18S and 5S rDNA sites. The occurrence of 18S/5S rDNA syntenic arrangements in all the species indicates a functionally stable and adaptive genomic organization. The phylogenetic sharing of this rDNA organization highlights a marked and unusual chromosomal singularity inside the family Labridae.

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“…The sequences of 5S primers were designed according to Komiya and Takemura [ 53 ]: and ; and those of the 28S primers were: and [ 54 ]. The temperature profile for the amplification of the 5S locus was: initial denaturation step for 5 min at 95°C, followed by 34 cycles (95°C for 15 s, 55°C for 30 s and 72°C for 30 s) and a final extension step at 72°C for 5 min [ 55 ] slightly modified by [ 56 ]. Conditions for PCR of the 28S locus were as follows: initial denaturation step for 3 min at 94°C, followed by 33 cycles (94°C for 30 sec, 53°C for 30 sec and 72°C for 45 sec) with final extension step at 72°C for 10 min [ 57 ] and modified by [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L

et al. 2018

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The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a higher ploidy level than the others. We therefore applied a set of molecular genetic (S7 nuclear and cytochrome b mitochondrial genes) and cytogenetic tools (sequential fluorescent 4’, 6-diamidino-2-phenylindole [DAPI], Chromomycin A3 [CMA3], C-banding and in situ hybridization [FISH] with both 5S and 28S ribosomal DNA probes) to determine its origin. While all examined characteristics of a diploid representative male (CCAHe2Fi) clearly corresponded to those of C. carassius, a triploid individual (CCAHe1Fi) was more complex. Phylogenetic analysis revealed that the nuclear genome of CCAHe1Fi contained three haploid sets: two C. gibelio and one C. carassius. However the mitochondrial DNA was that of C. gibelio, demonstrating its hybrid origin. The FISH revealed three strong (more intensive) 5S rDNA loci, confirming the triploid status, and an additional 24 weak (less intensive) signals were observed in the chromosome complement of CCAHe1Fi. On the other hand, only two strong and 16 weak 5S rDNA signals were visible on the chromosomes of the CCAHe2Fi male. 28S rDNA FISH revealed four strong signals in both CCAHe1Fi and CCAHe2Fi individuals. CMA3 staining revealed four to six CMA3-positive bands of CCAHe1Fi, while that of diploids contained only two to four. The fact that a polyploid hybrid Carassius female with a strong invasive potential may share morphological characters typical for endangered C. carassius highlights a need to combine genetic investigations of Carassius cryptic diversity with conservation measures of C. carassius in Europe.

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5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers

Cited by 6 publications

References 27 publications

First approach to studying the genetics of the meagre (Argyrosomus regius; Asso, 1801) using three multigene families

First approach to studying the genetics of the meagre (Argyrosomus regius; Asso, 1801) using three multigene families

Co-located 18S/5S rDNA arrays: an ancient and unusual chromosomal trait in Julidini species (Labridae, Perciformes)

Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L

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