Expression of D-type cyclins in differentiating cells of the mouse spinal cord

Salles, Patricy de Andrade; Fortes, Juliana Chagas; Guedes, Maria Izabel Florindo; Weller, M.

doi:10.1590/s1415-47572007000400032

Cited by 2 publications

(1 citation statement)

References 43 publications

(45 reference statements)

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“…Here, for example, Celsr1 , Frzb (also known as Sfrp3 ) and Sfrp5 (or its homologs Sfrp1/2 ) functional studies revealed that their inhibition is linked to enhanced stem cell maintenance (Boucherie [ 82 – 85 ]. Vice versa, we could detect upregulated proliferation-enhancing genes of the Wnt pathway like Ccnd2 known to be expressed during midbrain development [ 86 – 88 ]. We thus conclude that known proliferative effects on midbrain NSC through physioxia might be mediated through Wnt pathways and differ compared to cortical cells.…”

Section: Discussionmentioning

confidence: 99%

Serial Gene Expression Profiling of Neural Stem Cells Shows Transcriptome Switch by Long-Term Physioxia from Metabolic Adaption to Cell Signaling Profile

Braunschweig

Lanto

Meyer

et al. 2022

Stem Cells International

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Oxygen is an essential factor in the cellular microenvironment with pivotal effects on neural development with a particular sensitivity of midbrain neural stem cells (NSCs) to high atmospheric oxygen tension. However, most experiments are still performed at atmospheric O2 levels (21%, normoxia), whereas mammalian brain tissue is physiologically exposed to substantially lower O2 tensions around 3% (physioxia). We here performed serial Affymetrix gene array analyses to detect expression changes in mouse fetal NSCs from both midbrain and cortical tissues when kept at physioxia compared to normoxia. We identified more than 400 O2-regulated genes involved in cellular metabolism, cell proliferation/differentiation, and various signaling pathways. NSCs from both regions showed a low number but high conformity of regulated genes (9 genes in midbrain vs. 34 in cortical NSCs; 8 concordant expression changes) after short-term physioxia (2 days) with metabolic processes and cellular processes being the most prominent GO categories pointing to cellular adaption to lower oxygen levels. Gene expression profiles changed dramatically after long-term physioxia (13 days) with a higher number of regulated genes and more diverse expression patterns when comparing the two NSC types (338 genes in midbrain vs. 121 in cortical NSCs; 75 concordant changes). Most prominently, we observed a reduction of hits in metabolic processes but an increase in biological regulation and signaling pointing to a switch towards signaling processes and stem cell maintenance. Our data may serve as a basis for identifying potential signaling pathways that maintain stem cell characteristics in cortical versus midbrain physioxic stem cell niches.

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Section: Discussionmentioning

confidence: 99%

Serial Gene Expression Profiling of Neural Stem Cells Shows Transcriptome Switch by Long-Term Physioxia from Metabolic Adaption to Cell Signaling Profile

Braunschweig

Lanto

Meyer

et al. 2022

Stem Cells International

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Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro

Shaker

Kim

et al. 2015

Stem Cells and Development

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Secondary neurulation is an embryonic progress that gives rise to the secondary neural tube, the precursor of the lower spinal cord region. The secondary neural tube is derived from aggregated Sox2-expressing neural cells at the dorsal region of the tail bud, which eventually forms rosette or tube-like structures to give rise to neural tissues in the tail bud. We addressed whether the embryonic tail contains neural stem cells (NSCs), namely secondary NSCs (sNSCs), with the potential for self-renewal in vitro. Using in vitro neurosphere assays, neurospheres readily formed at the rosette and neural-tube levels, but less frequently at the tail bud tip level. Furthermore, we identified that sNSC-generated neurospheres were significantly smaller in size compared with cortical neurospheres. Interestingly, various cell cycle analyses revealed that this difference was not due to a reduction in the proliferation rate of NSCs, but rather the neuronal commitment of sNSCs, as sNSC-derived neurospheres contain more committed neuronal progenitor cells, even in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). These results suggest that the higher tendency for sNSCs to spontaneously differentiate into progenitor cells may explain the limited expansion of the secondary neural tube during embryonic development.

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Expression of D-type cyclins in differentiating cells of the mouse spinal cord

Cited by 2 publications

References 43 publications

Serial Gene Expression Profiling of Neural Stem Cells Shows Transcriptome Switch by Long-Term Physioxia from Metabolic Adaption to Cell Signaling Profile

Serial Gene Expression Profiling of Neural Stem Cells Shows Transcriptome Switch by Long-Term Physioxia from Metabolic Adaption to Cell Signaling Profile

Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro

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