Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico

Perales-Flores, Laura E.; Sifuentes‐Rincón, Ana María; León, Francisco J. Garcı́a de

doi:10.1590/s1415-47572007000400011

Cited by 9 publications

(19 citation statements)

References 25 publications

(26 reference statements)

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“…These results could be a consequence of empirically mixing selection broodstock from other hatcheries, which is a common practice on traditional farms. As previously reported by Perales-Flores et al (2007), this practice maintains genetic diversity in the genetic pool as a whole.…”

Section: Resultsmentioning

confidence: 54%

“…All of the samples were analyzed at 11 microsatellite loci (Table 1), as previously described (Perales-Flores et al 2007;Waldbieser and Wolters, 2007). PCR reactions were performed in a final volume of 10 µL composed of 50 ng DNA, 1.25 U Taq DNA polymerase (Promega Co, Madison, WI, USA) and 0.5 mM dNTPs.…”

Section: Methodsmentioning

confidence: 99%

“…In some cases, these problems are related to the loss of genetic variability and inbreeding depression. Molecular technologies have been proposed and partially applied as versatile tools for monitoring genetic variability in regional populations (Perales-Flores et al 2007). Although producers face technical and basic issues that prevent the complete acceptance of these tools, some producers have invested time and money in monitoring genetic variability.…”

Section: Introductionmentioning

confidence: 99%

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Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico

Parra‐Bracamonte¹,

Sifuentes‐Rincón²,

Rosa-Reyna³

et al. 2011

Electro Journal of Biotech

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Background: Channel catfish are one of the most important aquaculture species raised for food purposes in Mexico. Two temporal samples were obtained from the largest channel catfish breeding hatchery in Mexico to identify changes in genetic diversity and inbreeding that are promoted by traditional hatchery management. Results: The genetic parameter analysis of 11 microsatellite loci showed no significant change in genetic diversity (p > 0.05). However, a significant heterozygosis deficiency was detected (p < 0.001), and genetic structure analysis indicated moderate differentiation between the temporally divided populations (FST = 0.08). A moderate level of inbreeding and a slight increase of the inbreeding coefficient from 0.23 to 0.27 were the result of traditional hatchery practices. To achieve an effective population size, the temporal approach resulted in a limited number of breeders to maintain genetic variability. Conclusions: Although no significant change in genetic diversity parameters was found, the heterozygote deficiency and low effective number of breeders suggest that there is a risk for increased inbreeding. Thus, we propose the need for controlled reproductive management and the establishment of genetic programs in hatcheries. Molecular tools can provide valuable information to facilitate the achievement of these goals.

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Section: Resultsmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico

Parra‐Bracamonte¹,

Sifuentes‐Rincón²,

Rosa-Reyna³

et al. 2011

Electro Journal of Biotech

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“…In recent years, genetic techniques have been found to be more powerful in analysing population structure in salmonid strains (Clifford et al 1998) and acipenserid fishes (King et al 2001, Fopp-Bayat 2004, 2007. There are numerous studies that clearly demonstrate loss of genetic diversity following hatchery culture in aquatic organisms, for example in Salmo salar (see Norris et al 1999), Cyprinus carpio (see Desvignes et al 2001, Bártfai et al 2003, or Ictalurus punctatus (see Perales-Flores 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The use of microsatellite DNA as high polymorphic DNA markers has gained in importance for a number fish species over the past decade owing to the fact that it is an easy and powerful method (Norris et al 1999, King et al 2001, Fopp-Bayat 2004, Perales-Flores et al 2007). The microsatellite, or short sequence repeat (SSR), is a powerful genetic marker, useful in many areas of fish genetics and breeding.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite DNA Variation in the Siberian Sturgeon, <I>Acipenser Baeri</I> (Actinopterygii, Acipenseriformes, Acipenseridae), Cultured in a Polish Fish Farm

Fopp‐Bayat¹

2010

Acta Icth et Piscat

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Fopp-Bayat D. 2010. Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), cultured in a Polish fish farm. Acta Ichthyol. Piscat. 40 (1): 21-25.Background. The Siberian sturgeon, Acipenser baeri, is one of the most important sturgeon species cultured in Poland. The effective management of aquaculture production of this species requires contemporary knowledge of broodstock structure, mating patterns, and genetic diversity of broodstock. The aim of the present study was the application of microsatellite DNA analysis for estimation of gene diversity in the Siberian sturgeon farmed at a Polish fish farm. Materials and Methods. Fin clips were randomly sampled from 94 specimens of Siberian sturgeon broodstock reared at the Wąsosze Fish Farm near Konin, Poland. The analysed broodstock has been kept there since 1996, with new specimens being introduced annually. The fish were studied in 2007-2008. Genomic DNA for amplification of microsatellite loci was extracted using Chelex 100. Six microsatellite loci were amplified for examination of the genetic variability of the studied fish.Results. Within 94 individuals of the Siberian sturgeon, a total of 74 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 8 to 18, with an average allele number being 12.The genetic diversity of six microsatellite loci varied from 0.686 to 0.811. Conclusion. This technology has great potential for use in aquaculture of sturgeon fish, especially when levels of genetic variation could be monitored and inbreeding controlled in commercial breeding programs.

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Estimating the genetic diversity and potential influence of habitat segregation in Channel Catfish

Janzen,

Blouin‐Demers

2023

Trans Am Fish Soc

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ObjectiveIndividual habitat preference can reduce intraspecific competition for resources and may differ between age groups, sexes, and adult phenotypes. The Channel Catfish Ictalurus punctatus is a widespread species occurring in diverse freshwater habitats. This species displays breeding philopatry, returning to nesting sites occupied in previous years. Larger Channel Catfish tend to nest in the main channels of large rivers, whereas smaller fish tend to prefer smaller tributaries. The purpose of our study was to determine whether this habitat segregation potentially associated with habitat preference affects the genetic structure of a population. We hypothesized that spatial segregation of breeding sites in the Ottawa River and its smaller tributaries at Lac des Chats reduced gene flow within the population, resulting in genetically differentiated demes associated with lacustrine‐like and fluvial habitats.MethodsMicrosatellite allelic data was collected from 162 Channel Catfish.ResultWe found little genetic variation between the Ottawa, Mississippi, and Madawaska rivers. Furthermore, our analyses suggested that the sampled specimens comprised one panmictic population. Fish from one site in the Ottawa River, however, were significantly differentiated from fish from a nearby site also in the Ottawa River as well as from fish from the Mississippi River tributary.ConclusionGiven that fish from sites further up the Ottawa River were not differentiated from fish from these sites, it is unlikely that geography can account for the differences observed; rather, assortative mating may explain the differentiation. We propose that panmixia within the population is caused by ontogenetic changes in habitat selection, straying individuals, or sex‐biased dispersal and philopatry.

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Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico

Cited by 9 publications

References 25 publications

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico

Microsatellite DNA Variation in the Siberian Sturgeon, <I>Acipenser Baeri</I> (Actinopterygii, Acipenseriformes, Acipenseridae), Cultured in a Polish Fish Farm

Estimating the genetic diversity and potential influence of habitat segregation in Channel Catfish

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