Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae)

Vicari, Marcelo Ricardo; Almeida, Mara Cristina de; Bertollo, Luiz Antônio Carlos; Moreira‐Filho, Orlando; Artoni, Roberto Ferreira

doi:10.1590/s1415-47572006000400008

Cited by 26 publications

(29 citation statements)

References 30 publications

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“…population, is suggested as a conserved condition for different fish groups (Jesus & Moreira-Filho, 2003;Hatanaka & Galetti, 2004;Vicari et al, 2006). Almeida-Toledo et al (2002) observed similar details to the present study, with an 5S rDNA site co-localized with 28S rDNA in two acrocentric pairs, in a population of Astyanax paranae of the rio Tietê and in a population of A. aff.…”

Section: Discussionsupporting

confidence: 90%

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)

et al. 2014

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Two populations of the Astyanax scabripinnis complex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the absence of B chromosomes in the lower altitude population. The fluorescence in situ hybridization showed differences for both markers (18S rDNA and 5S rDNA), and reproductive data suggests pre-zygotic reproductive isolation among the two populations. The data showed the absence of gene flow, indicating that an incipient speciation process has occurred, which leads the two populations to follow independent evolutionary pathways.Duas populações do complexo Astyanax scabripinnis isoladas por uma queda d´água de mais de 100 metros de altura e localizadas em diferentes altitudes do mesmo rio (662 m e 1850 m a.s.l.) foram comparadas através de dados de reprodução, cariótipo, morfometria geométrica, morfologia dentária, e número de raios da nadadeira anal, de modo a testar a hipótese de especiação entre as duas populações. Os resultados de morfometria geométrica mostraram diferenças entre as populações. A análise da função discriminante (DFA) e a análise de variância canônica (CVA) demonstraram a presença de dimorfismo sexual. Caracteres sexuais secundários, como ganchos em raios da nadadeira anal dos machos, estão ausentes na população de menor altitude. Ambas as populações têm a mesma macro estrutura cariotípica, exceto pela ausência de cromossomos B na população de menor altitude. A hibridação in situ mostrou diferenças para ambos os marcadores (rDNA 18S e rDNA 5S), e os dados de reprodução sugerem isolamento reprodutivo pré-zigótico entre as duas populações. Os dados mostram ausência de fluxo gênico, indicando que ocorreu um processo de especiação incipiente, o que leva as duas populações seguirem vias evolutivas independentes.

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Section: Discussionsupporting

confidence: 90%

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)

et al. 2014

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“…Heteromorphisms of NORs are very common in fish and they are likely determined by unequal cross-over, gene duplication, transposition, or other rearrangements involving homologous chromosomal segments (Borin and Martins-Santos, 1999;Vicari et al, 2003Vicari et al, , 2006. Such NOR heteromorphic condition has already been formerly reported in a population of I. labrosus from the Capivara reservoir, Paraná, Brazil (Carvalho and Dias, 2007).…”

Section: Discussionmentioning

confidence: 94%

Karyotypic variability in Iheringichthys labrosus (Teleostei, Pimelodidae) from the Tibagi River basin (Paraná State, Brazil)

Ribeiro

Matoso²,

Almeida³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Cytogenetic analyses were carried out in a populational sample of Iheringichthys labrosus from the Guaraúna River (Upper Tibagi River; Paraná State, Brazil) in order to provide a karyotypic comparison with another previously studied population from the Lower Tibagi River, characterized by the presence of 32m + 8sm + 6st + 10a (2n = 56, FN = 102) and occurrence of supernumerary chromosomes (80% of individuals). The 17 specimens of I. labrosus (6 females, 10 males and 1 of unknown sex) from the Upper Tibagi River showed 2n = 56 chromosomes, a karyotype formula of 14m + 32sm + 4st + 6a (FN = 106), without evidence of sex chromosome heteromorphism or supernumerary chromosomes. The heterochromatin was detected Karyotypic variability in Iheringichthys labrosusat telomeric and centromeric positions in several chromosomal pairs. The Ag-nucleolar organizer regions were heteromorphic and located at terminal position on short arms of the 16th chromosomal pair, suggesting a positive association with heterochromatic regions. The inter-populational karyotypic differentiation reported indicates distinct evolutionary pathways within I. labrosus in the Tibagi River basin.

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“…The polymorphism of the NORs is a relatively common event in Neotropical fish (e.g. Foresti et al, 1981;Brum et al;Vicari et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

“…The occurrence of 18S and 5S ribosomal DNA sites in synteny is rare in vertebrates and has only been reported in a few organisms, such as the fish species Salmo salar (Linnaeus, 1758) (Pendás et al, 1994), Oncorhynchus mykiss (Walbaum, 1792) (Moran et al, 1996), Prochilodus argenteus (Hatanaka & Galetti, 2004), Prochilodus lineatus (Valenciennes, 1836) (Jesus et al, 2003;Vicari et al, 2006), five species of the genus Astyanax (Almeida-Toledo et al, 2002) and amphibians (Lucchini et al, 1993). Moreover, the loci have been mapped on different chromosomes in several fish species (Morán et al, 1996;Wasko et al, 2001), a condition often found in vertebrates (Lucchini et al, 1993;Suzuki et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)

et al. 2012

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Cytogenetic and molecular analyses were carried out in fish representative of the genus Piabina. This study specifically involved the species P. argentea and P. anhembi collected from areas of the Paranapanema and Tietê River basins, Brazil. Our findings suggest that fish classified as Piabina argentea in the Paranapanema and Tietê Rivers may represent more than one species. The samples analyzed differed by cytogenetic particularities and molecular analyses using partial sequences of the genes COI and CytB as genetic markers revealed three distinct groups of P. argentea with genetic distances sufficient to support the conclusion that the three samples analyzed are three distinct taxonomic units.Foram realizadas análises citogenéticas e moleculares em representantes do gênero Piabina. O estudo envolveu especificamente as espécies P. argentea e P. anhembi coletadas nas áreas das bacias hidrográficas dos rios Paranapanema e Tietê (Brasil). Os dados sugerem que a espécie P. argentea coletada nas bacias dos rios Paranapanema e Tietê podem representar mais de uma espécie. As amostras analisadas diferem por particularidades citogenéticas e nas análises moleculares utilizando-se sequências parciais dos genes COI e CytB, revelando três grupos distintos de P. argentea com distâncias genéticas suficientes para sustentar a conclusão de que as três amostras analisadas são unidades taxonômicas distintas.

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Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae)

Cited by 26 publications

References 30 publications

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)

Karyotypic variability in Iheringichthys labrosus (Teleostei, Pimelodidae) from the Tibagi River basin (Paraná State, Brazil)

Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)

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