Low doses of gamma ionizing radiation increase hprt mutant frequencies of TK6 cells without triggering the mutator phenotype pathway

Ayres, Flávio Monteiro; Cruz, Aparecido Divino da; Steele, Patricia P.; Glickman, Barry W.

doi:10.1590/s1415-47572006000300027

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…To evaluate the genotoxic potential of PEPs, a DNA damage assessment was performed on human lymphoblasts (TK6 cells), which are genetically sensitive to chemical exposure ( Ayres et al 2006 ; Kimura et al 2013 ). The results from the Nano-CometChip assay indicate that PEPs did not inflict significant DNA damage on the lymphoblasts (see Supplemental Material, Figure S4).…”

Section: Resultsmentioning

confidence: 99%

Effects of Laser Printer–Emitted Engineered Nanoparticles on Cytotoxicity, Chemokine Expression, Reactive Oxygen Species, DNA Methylation, and DNA Damage: A Comprehensive in Vitro Analysis in Human Small Airway Epithelial Cells, Macrophages, and Lymphoblasts

Pirela

Miousse

et al. 2016

Environ Health Perspect

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BackgroundEngineered nanomaterials (ENMs) incorporated into toner formulations of printing equipment become airborne during consumer use. Although information on the complex physicochemical and toxicological properties of both toner powders and printer-emitted particles (PEPs) continues to grow, most toxicological studies have not used the actual PEPs but rather have primarily used raw toner powders, which are not representative of current exposures experienced at the consumer level during printing.ObjectivesWe assessed the biological responses of a panel of human cell lines to PEPs.MethodsThree physiologically relevant cell lines—small airway epithelial cells (SAECs), macrophages (THP-1 cells), and lymphoblasts (TK6 cells)—were exposed to PEPs at a wide range of doses (0.5–100 μg/mL) corresponding to human inhalation exposure durations at the consumer level of 8 hr or more. Following treatment, toxicological parameters reflecting distinct mechanisms were evaluated.ResultsPEPs caused significant membrane integrity damage, an increase in reactive oxygen species (ROS) production, and an increase in pro-inflammatory cytokine release in different cell lines at doses equivalent to exposure durations from 7.8 to 1,500 hr. Furthermore, there were differences in methylation patterns that, although not statistically significant, demonstrate the potential effects of PEPs on the overall epigenome following exposure.ConclusionsThe in vitro findings obtained in this study suggest that laser printer–emitted engineered nanoparticles may be deleterious to lung cells and provide preliminary evidence of epigenetic modifications that might translate to pulmonary disorders.CitationPirela SV, Miousse IR, Lu X, Castranova V, Thomas T, Qian Y, Bello D, Kobzik L, Koturbash I, Demokritou P. 2016. Effects of laser printer–emitted engineered nanoparticles on cytotoxicity, chemokine expression, reactive oxygen species, DNA methylation, and DNA damage: a comprehensive in vitro analysis in human small airway epithelial cells, macrophages, and lymphoblasts. Environ Health Perspect 124:210–219; http://dx.doi.org/10.1289/ehp.1409582

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Section: Resultsmentioning

confidence: 99%

Effects of Laser Printer–Emitted Engineered Nanoparticles on Cytotoxicity, Chemokine Expression, Reactive Oxygen Species, DNA Methylation, and DNA Damage: A Comprehensive in Vitro Analysis in Human Small Airway Epithelial Cells, Macrophages, and Lymphoblasts

Pirela

Miousse

et al. 2016

Environ Health Perspect

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“…They also reported that the large lesions were radiation dose dependent, whereas ‘other lesions (events excluding large lesions)’ did not depend on either the radiation dose or the dose rate. In addition, there is a report showing that low-dose gamma-irradiation (>0.2 Gy) increases the Hprt-deficient mutant frequency without triggering mutator phenotype pathways depending on mismatch repair [ 24 ]. These findings support our results showing that there are radiation-associated events leading to mutagenesis.…”

Section: Discussionmentioning

confidence: 99%

Induction of somatic mutations by low-dose X-rays: the challenge in recognizing radiation-induced events

Nagashima

Shiraishi

Ohkawa

et al. 2017

Journal of Radiation Research

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It is difficult to distinguish radiation-induced events from spontaneous events during induction of stochastic effects, especially in the case of low-dose or low-dose-rate exposures. By using a hypersensitive system for detecting somatic mutations at the HPRT1 locus, we investigated the frequency and spectrum of mutations induced by low-dose X-rays. The mutant frequencies induced by doses of >0.15 Gy were statistically significant when compared with the spontaneous frequency, and a clear dose dependency was also observed for mutant frequencies at doses of >0.15 Gy. In contrast, mutant frequencies at doses of <0.1 Gy occurred at non-significant levels. The mutation spectrum in HPRT-deficient mutants revealed that the type of mutations induced by low-dose exposures was similar to that seen in spontaneous mutants. An apparent change in mutation type was observed for mutants induced by doses of >0.2 Gy. Our observations suggest that there could be a critical dose for mutation induction at between 0.1 Gy and 0.2 Gy, where mutagenic events are induced by multiple DNA double-strand breaks (DSBs). These observations also suggest that low-dose radiation delivered at doses of <0.1 Gy may not result in DSB-induced mutations but may enhance spontaneous mutagenesis events.

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“…A lot of studies have reported an increase the mutation frequency of HPRT gene along with other genetic aberration in several radiation accidents or occupational exposure. Ayres and colleagues have reported that low dose ionizing radiation exposure associated with increased HPRT mutation frequency without triggering the mutator phenotype pathway [11]. HPRT mutations are also linked to cancer development due to its role in cell cycle regulation [12].…”

Section: Introductionmentioning

confidence: 99%

Investigation of HPRT Sequence Changes in Radiation Occupationally-Exposed Workers-in Al-Twuathia Site in Baghdad City

Haider- Y-Lafta,

Risala H –Allami,

Naemh- H- Zghair

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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The present instruction targets according to makes use of the molecular organic strategies into a genotoxicity looking after about mean diffraction doses within samples concerning people among Al-Twuathia site, it learning inclusive of 35♂ blood samples, used (30 -fifty five year), so well as 15 ♂blood samples, ancient (25 -50 year) permanency toughness which are no longer people who smoke yet alcohol as much control. In that research usage DNA sequences technique for investigating the factor mutations appropriate in conformity with the impact regarding ignoble doses on ionizing radiation within the blood of smearing workers namely nicely namely the discovery about the effect concerning ionizing diffraction on the genes The genetic susceptibility after smearing exposure is in all likelihood after end result from the contribution about much genetic variants between multiple repair pathways as may bear a suture then additive impact over repair diverse DNA damages. Longevity a more comprehensive, larger-scale genetic instruction is needed after similarly explore the outcomes about gene-environment interaction concerning genetic susceptibility in conformity with ionizing dispersion exposure. In conclusion, the results indicated the opportunity over the use of the adjustments of as like beneficial biomarkers for the discovery concerning ethnical publicity in accordance with ionizing radiation., the learnt tested usefulness on the mutation frequency because HPRT assay then DNA sequences technique namely a sensitive extra biomarker among the everyday fitness screening about people occupationally uncovered to low doses on ionizing diffraction the effects indicated as at that place is an opportunity over the usage of the changes at the degree concerning HPRT gene mutation as much useful biomarkers because of the detection of ethnic exposure in accordance with radiological or chemical substances yet in accordance with makes use of PCR Techniques as like biomarkers because detection about the consequences on the low doses painting within the lymphocytes of gore among people occupationally to ionizing diffraction at the Al-Twuathia site.

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Low doses of gamma ionizing radiation increase hprt mutant frequencies of TK6 cells without triggering the mutator phenotype pathway

Cited by 5 publications

References 19 publications

Effects of Laser Printer–Emitted Engineered Nanoparticles on Cytotoxicity, Chemokine Expression, Reactive Oxygen Species, DNA Methylation, and DNA Damage: A Comprehensive in Vitro Analysis in Human Small Airway Epithelial Cells, Macrophages, and Lymphoblasts

Effects of Laser Printer–Emitted Engineered Nanoparticles on Cytotoxicity, Chemokine Expression, Reactive Oxygen Species, DNA Methylation, and DNA Damage: A Comprehensive in Vitro Analysis in Human Small Airway Epithelial Cells, Macrophages, and Lymphoblasts

Induction of somatic mutations by low-dose X-rays: the challenge in recognizing radiation-induced events

Investigation of HPRT Sequence Changes in Radiation Occupationally-Exposed Workers-in Al-Twuathia Site in Baghdad City

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