A microsatellite study of bovine solute carrier family 11 a1 (Slc11a1) gene diversity in Mexico in relation to bovine tuberculosis

Vázquez-Flores, Felicitas; Alonso, Ricardo; Villegas‐Sepúlveda, Nicolás; Arriaga, C.; Pereira‐Suárez, Ana Laura; Mancilla, Raúl; Estrada-Chávez, Ciro

doi:10.1590/s1415-47572006000300019

Cited by 5 publications

(2 citation statements)

References 18 publications

(14 reference statements)

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“…A study in Taiwanese HF cattle identified SNPs in exon 4 and intron 4 that were associated with enhanced susceptibility to bTB ( Cheng et al, 2015 ), while in a Chinese HF cattle population, associations with bTB susceptibility/resistance were found with SNPs in exon 11 and introns 5 and 9 ( Liu et al, 2017 ). Additionally polymorphisms in the 3′UTR of the bovine SLC11A1 gene, that have been shown to cause changes to the lengths of two microsatellites (MS1 and MS2), have been identified in various breeds of cattle ( Paixao et al, 2006 ; Vazquez-Flores et al, 2006 ; Hasenauer et al, 2013 ). Interestingly, an increase in the length of the MS1 microsatellite has been described to reduce traits indicative of bTB infection in African zebu cattle ( Kadarmideen et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

et al. 2020

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Bovine tuberculosis (bTB), caused by Mycobacterium bovis , is a chronic zoonotic disease where host genetics is thought to contribute to susceptibility or resistance. One of the genes implicated is the SLC11A1 gene, that encodes for the natural resistance-associated macrophage protein 1 (NRAMP1). The aim of this study was to identify SLC11A1 polymorphisms and to investigate any resulting functional differences in NRAMP1 expression that might be correlated with resistance/susceptibility to M. bovis infection. Sequencing of the SLC11A1 gene in cDNA isolated from Brown Swiss, Holstein Friesian, and Sahiwal cattle identified five single nucleotide polymorphisms (SNPs) in the coding region, but only one of these (SNP4, c.1066C>G, rs109453173) was present in all three cattle breeds and therefore warranted further investigation. Additionally, variations of 10, 11, and 12 GT repeats were identified in a microsatellite (MS1) in the SLC11A1 3′UTR. Measurement of NRAMP1 expression in bovine macrophages by ELISA showed no differences between cells generated from the different breeds. Furthermore, variations in the length of the MS1 microsatellite did not impact on NRAMP1 protein expression as analyzed by luciferase reporter assay. However, further analysis of the ELISA data identified that the presence of the alternative G allele at SNP4 was associated with increased expression of NRAMP1 in bovine macrophages. Since NRAMP1 has been shown to influence the survival of intracellular pathogens such as M. bovis through the sequestering of iron, it is possible that cattle expressing the alternative G allele might have an increased resistance to bTB through increased NRAMP1 expression in their macrophages.

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Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

et al. 2020

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“…Interestingly, in cattle it has been demonstrated that differences in susceptibility to BTB is at the level of genus [11], indicating that Bos indicus cattle are more resistant than Bos taurus. Although there is no particular gene known to be responsible for differences in BTB infection susceptibility in cattle, natural resistant associated macrophage protein 1 (NRAMP1) was a known candidate gene; it is associated with natural resistance to infection with Mycobacterium spp., in cattle [12,13]. Recently, a genome-wide association study on Holstein-Friesian herds in Irish cattle that used the bovine 50 k SNP chip has identified three SNPs in a 65 kb genomic region on chromosome 22 associated with BTB susceptibility [14].…”

Section: Introductionmentioning

confidence: 99%

Association between microsatellite markers and bovine tuberculosis in Chadian Zebu cattle

Ali¹,

Thomson²,

Kadarmideen³

2013

OJAS

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Bovine tuberculosis (BTB) is a considerable threat to livestock keepers and public health in many developing and underdeveloped countries. We investigated associations between 20 microsatellite markers and three phenotypes for BTB in a sample of the Chadian cattle population. The phenotypes measured were: 1) single intra-dermal comparative cervical tuberculin test (SICCT) performed on live animals, 2) presence of lesion post-slaughter, 3) a bacteriological tissue culture test for Mycobacterium bovis using the samples with observed lesions and 4) a predicted Bayesian model (BM) estimate of a true BTB disease status using all tested animals. All traits were recorded in binary form and as either 1 = presence or 0 = absence. A total of 224 animals for SICCT, lesion and BM traits and 96 animals with bacteriological culture test were genotyped. Generalised linear models were fitted to the binary BTB phenotypes that consisted of age (covariate), sex (2 levels), breed (2 levels) and markers (alleles: 5 -14 levels) as explanatory variables and implemented in R using glm with a logit link function. The model was fitted for each marker, separately. Six out of 20 markers tested were significantly associated with at least one trait considered; these were ILSTS005, ILSTS006, TGLA227, BM2113 and CSRM66. Genomic regions around these markers may serve as a basis for further functional investigations. This is the first study to report association of microsatellite markers with bovine tuberculosis traits in African or Chadian cattle population.

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Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus

Mohindra¹,

Singh²,

Barman³

et al. 2011

Mol Biol Rep

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Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained, out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes.

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A microsatellite study of bovine solute carrier family 11 a1 (Slc11a1) gene diversity in Mexico in relation to bovine tuberculosis

Cited by 5 publications

References 18 publications

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

Association between microsatellite markers and bovine tuberculosis in Chadian Zebu cattle

Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus

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