Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures

José, A A F B V; Alvares, Lúcia Elvira; Delgado, Eduardo Francisquine; Coutinho, Luiz Lehmann; Lanna, Dante Pazzanese Duarte

doi:10.1590/s1415-47572006000100032

Cited by 4 publications

(3 citation statements)

References 42 publications

(70 reference statements)

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“…In contrast, Choi et al (2000) observed no alterations in FAS mRNA expression when immature 3T3-L1 adipocytes were cultured with trans-10, cis-12 CLA. In a previous study, where the effects of pGH were evaluated under the same conditions, José et al (2006) observed a reduction of 22% in FAS enzymatic activity, which was accompanied by 70% of reduction in mRNA abundance after 48 h of incubation. In the present study, the effects were similar in direction, but of different magnitude.…”

Section: Effect Of Trans-10 Cis-12 Cla On Lipid Metabolism Results Amentioning

confidence: 79%

Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

José

Gama²,

Lanna³

2008

Genet. Mol. Res.

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ABStrAct. The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14 C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA Effect of trans-10, cis-12 CLA on lipid metabolism had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.

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Section: Effect Of Trans-10 Cis-12 Cla On Lipid Metabolism Results Amentioning

confidence: 79%

Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

José

Gama²,

Lanna³

2008

Genet. Mol. Res.

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“…In growing swine, 80% of fatty acid is a result of de novo synthesis, using glucose as substrate, because only a low proportion of calories in their normal feed is present as fatty acids, and about 40% body glucose usage is destined for fat deposition in adipose tissue (José et al, 2006). The addition of glycerin reduced corn inclusion in the experimental diets and, therefore, lower the substrate for synthesis and transport of fatty acids by the liver, suggesting a reduction of circulating cholesterol and triglycerides.…”

Section: Serum Biochemical Indicatorsmentioning

confidence: 99%

Performance and serum parameters of growing pigs fed semi- purified glycerin

Verussa

Corassa

Pina

et al. 2017

Rev Colomb Cienc Pecu

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Performance and serum parameters of growing pigs fed semipurified glycerin ¤ Desempeño productivo y parámetros séricos de cerdos en crecimiento alimentados con glicerina semipurificada Desempenho e parâmetros séricos de suínos em crescimento alimentados com glicerina semipurificada Rei, Praça Frei Orlando, 170, Centro, CEP 36307-352, São João Del-Rei, MG, Brasil. (Received: November 16, 2015; accepted: August 16, 2016) AbstractBackground: the use of semi-purified glycerin in pig diets has been studied as an alternative energy source. Objective: to evaluate growth performance and serum levels of glucose, triglycerides, cholesterol, and urea in pigs fed diets containing semi-purified glycerin during the growth phase. Methods: forty barrows with an initial weight of 27.30 ± 1.74 Kg, distributed in a randomized complete block design with four treatments and five replicates were used. The dietary treatments were a combination of corn and soybean meal diets with the addition of 0, 5, 10 or 15% semi-purified glycerin. The experimental period lasted 27 days and was divided into two periods. Results: during Period 1, pig performance did not differ with the inclusion of semi-purified glycerin in the diet. In Period 2, an improvement in average daily weight gain was observed. In the total period, an improvement was observed in the average daily weight gain, feed/gain ratio and final weight of the animals. However, serum parameters were not affected by the inclusion of semi-purified glycerin in the diets. Conclusion: the addition of up to 15% semi-purified glycerin to the diet of growing pigs improves growth performance without affecting serum parameters.Keywords : cholesterol, co-product, glucose, glycerol, triglycerides, urea. 12Rev Colomb Cienc Pecu 2017; 30:11-20 Verussa GH et al. Glycerin for growing pigsResumen Antecedentes: el uso de la glicerina semi-purificada en la dieta de cerdos se ha estudiado como una fuente alternativa de energía. Objetivo: evaluar el desempeño y los niveles séricos de glucosa, triglicéridos, colesterol y urea en cerdos alimentados con dietas que contienen glicerina semi-purificada durante la fase de crecimiento. Métodos: fueron utilizados 40 machos castrados con un peso inicial de 27,30 ± 1,74 Kg, distribuidos en un diseño experimental en bloques al azar con cuatro tratamientos y cinco repeticiones. Los tratamientos se basaron en maíz y harina de soja con adición de 0, 5, 10 o 15% de glicerina semi-purificada. El período experimental duró 27 días, dividido en dos períodos. Resultados: en el período 1, el desempeño productivo de los cerdos no fue alterado por la inclusión de glicerina semi-purificada en la dieta. En el período 2 se observó una mejora en la ganancia diaria de peso. En el período total se observó una mejora en la ganancia diaria de peso, conversión alimenticia y el peso final de los animales. Los parámetros séricos no se afectaron por la inclusión de glicerina semi-purificada en la dieta. Conclusión: la adición de glicerina semi-purificada hasta en un 15% en la di...

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“…Reduction in FASN expression in adipose tissue and liver leads to increased lipid oxidation (211,212). Increased FASN expression in adipose tissue increases lipogenesis (213).…”

Section: 5! Discussionmentioning

confidence: 99%

The role of growth hormone in the regulation of the anaerobic energy system and physical function

Chikani¹

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Growth hormone (GH) regulates energy metabolism and body composition in adult life. Adults with GH deficiency (GHD) suffer from lack of energy and from impaired physical functioning. GH supplementation improves sprinting in recreational athletes, a performance measure dependent on the anaerobic energy system (AES). The AES underpins the initiation of all physical activities including those of daily living. The physiological and functional significance of GH in regulation of the AES is unknown.This thesis tests the hypothesis that GH positively regulates the AES and aspects of physical functioning in adult life. The key objectives are to 1) investigate whether anaerobic capacity is impaired in adults with GHD and improved by GH replacement, ii) characterise facets of physical function that are AES-dependent and GH responsive and iii) identify GH-regulated genes governing anaerobic metabolism in skeletal muscle.Exercise capacity, body composition, physical function and quality of life (QoL) were studied in 19 adults with GHD before and after GH replacement. Anaerobic capacity was assessed by the 30-second Wingate test, and aerobic capacity by the VO 2 max test. Physical function was assessed by the stair-climb test, chair-stand test, and 7-day pedometry. QoL was assessed by a GHD-specific questionnaire. Lean body mass (LBM) was quantified by dual-energy x-ray absorptiometry. Muscle biopsies were obtained before and after 1 and 6 months of GH replacement. GH responsive genes were identified by microarray analysis.In a cross-sectional study, anaerobic capacity and aerobic capacity were significantly reduced compared to age-, gender-and body mass index-matched normal adults. The duration of the stair climb test was longer, the number of chair stand repetitions and daily step counts were lower in adults with GHD who had lower QoL scores. GH status was an independent predictor of anaerobic capacity, which significantly and independently correlated with stair climb performance and QoL.One month of GH replacement (0.5 mg/day) did not significantly change anaerobic capacity nor any of the other outcome measures compared to placebo in adults with GHD. Six months of GH treatment significantly increased LBM, anaerobic capacity, chair-stand repetitions, daily step count, and QoL scores but not VO 2 max. Improvement in anaerobic capacity significantly correlated with an improvement in the energy and vitality domains of QoL. GH treatment did not significantly affect the differential expression of metabolic genes in skeletal muscle.In summary, anaerobic capacity, aerobic capacity, physical function and QoL were impaired in adults with GHD. GH replacement improved anaerobic capacity, LBM, chair-stand performance, daily step counts and QoL in a time-dependent manner without a concomitant improvement in aerobic capacity. GH at a replacement dose was insufficient to induce the detection of GH-regulated genes governing substrate metabolism and energy production in skeletal muscle.In conclusion, GH positively regulates the AES...

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Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures

Cited by 4 publications

References 42 publications

Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

Effects of trans-10, cis-12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs

Performance and serum parameters of growing pigs fed semi- purified glycerin

The role of growth hormone in the regulation of the anaerobic energy system and physical function

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