Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences

Inglis, Peter W.; Tigano, Myrian S.

doi:10.1590/s1415-47572006000100025

Cited by 66 publications

(49 citation statements)

References 15 publications

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“…Additionally, the morphological differences observed may be the product of simple mutations or media/cultivation [3]. Phenotypic characters of a fungal species often displayed a range of variation, which overlaps, with those of another species [4].…”

Section: Introductionmentioning

confidence: 99%

Species confirmation of fungal isolates by molecular analysis

Thakur¹,

Sandhu

2010

Indian J Microbiol

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Traditional taxonomy of hyphomycetes has been based on conidial morphology and development. In order to confi rm species level for the detection and identifi cation of the entomopathogenic fungus, we analysed the species-specifi c fi ngerprints to investigate molecular characteristics within isolates of six species and to resolve morphologically atypical isolates. The extent of fi ngerprint profi le observed by RAPD was suffi cient to confi rm the species level of all the isolates. The genetic similarity among morphologically identifi ed isolates of each species was considerably higher, allowing us to conclude that all the isolates are of same species. These results establish a molecular framework for further taxonomic, phylogenetic and comparative biological investigations.

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Section: Introductionmentioning

confidence: 99%

Species confirmation of fungal isolates by molecular analysis

Thakur¹,

Sandhu

2010

Indian J Microbiol

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“…Many other Paecilomyces species, including the pathogenic species P. lilacinus and P. viridis, group within the family Clavicipitaceae sensu lato (Hypocreales), comprising many arthropod-and grass-associated fungi (7,13,14). Some of these Paecilomyces species have been reclassified in the genus Isaria, but P. lilacinus and P. viridis are unrelated to Isaria species and their relationships to other members of the family have not been resolved (7,14). In a recent multigene phylogenetic study, clavicipitalean fungi were reclassified into three families, namely, Clavicipitaceae sensu stricto, Cordycipitaceae, and Ophiocordycipitaceae, but P. viridis was not examined in that study (20).…”

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confidence: 99%

“…The type species, P. variotii, is placed within the ascomycete family Trichocomaceae (Eurotiales) (12). Many other Paecilomyces species, including the pathogenic species P. lilacinus and P. viridis, group within the family Clavicipitaceae sensu lato (Hypocreales), comprising many arthropod-and grass-associated fungi (7,13,14). Some of these Paecilomyces species have been reclassified in the genus Isaria, but P. lilacinus and P. viridis are unrelated to Isaria species and their relationships to other members of the family have not been resolved (7,14).…”

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confidence: 99%

Disseminated Mycosis in Veiled Chameleons ( Chamaeleo calyptratus ) Caused by Chamaeleomyces granulomatis , a New Fungus Related to Paecilomyces viridis

et al. 2010

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An outbreak of disseminated granulomatous disease occurred in a group of veiled chameleons (Chamaeleo calyptratus) in a zoo collection. An adult female and six offspring developed large granulomas in multiple organs and were euthanized. At necropsy, roughly spherical yellow-to-white nodules 1 to 3 mm in diameter were grossly visible in the liver and other organs. Histopathology revealed fungal elements that were spherical to ovoid in shape, fragments of slender to irregularly swollen hyphae, and occasional conidia produced on phialides. Fungal isolates were initially suspected on the basis of morphology results to represent Paecilomyces viridis, a species known only from one outbreak of fatal mycosis in carpet chameleons (Furcifer lateralis). Data obtained from morphological studies and from phylogenetic analyses of nuclear ribosomal rRNA (rDNA) sequence data revealed the Danish chameleon isolates to be a related undescribed anamorphic species within the family Clavicipitaceae that includes many insect pathogens. Chamaeleomyces granulomatis gen. et sp. nov. is given as the name for the newly described fungus, and P. viridis is transferred to the new genus as Chamaeleomyces viridis comb. nov. Chamaeleomyces species are distinguished by having basally swollen phialides tapering to a narrow neck, conidia in fragile chains, and pale green to greenish-gray colonies. Both species are dimorphic, producing a transitory yeast stage characterized by ovoid-to-subglobose or subcylindrical yeast-like cells. Chamaeleomyces species appear to be rare but aggressive pathogens of chameleons.Disseminated, often fatal, mycoses caused by Paecilomyces species have previously been reported in many reptiles, including lizards and crocodiles; P. lilacinus and P. variotii are the main species involved (14). Paecilomyces viridis was first reported in 1964 as the cause of disseminated fungal infection in 4 of 50 carpet chameleons (Furcifer lateralis; formerly Chamaeleo lateralis) that were sent from Madagascar to Paris for experimental use (16,17). The fungus was isolated from blood and multiple organs, including liver and spleen, and it produced yeast-like cells in vivo but not in vitro. There have been no subsequent reports of P. viridis causing mycosis in any reptile, and two reports of human infection have not been well substantiated and probably concerned infections by P. variotii (4). A recent outbreak of disseminated granulomatous disease in a group of veiled chameleons (Chamaeleo calyptratus) housed at the Copenhagen Zoo yielded fungal isolates with morphologies resembling those of P. viridis. However, a DNA sequence from the internal transcribed spacer (ITS) region of the nuclear ribosomal rRNA (rDNA) gene from one isolate showed only 94% similarity to that of a P. viridis sequence obtained from GenBank (AY624194, strain CBS 348.65, extype culture). This result prompted further investigation into the relationship between P. viridis and the new chameleon isolates and other Paecilomyces species.Recent taxonomic studies have shown th...

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“…Other species reported to infect humans rarely include Paecilomyces marquandii and Paecilomyces javanicus 7 . Paecilomyces lilacinus, show poor susceptibility to amphotericin B, itraconazole, and echinocandins, while Paecilomyces variotii shows a different susceptibility pattern, being susceptible to most antifungal agents apart from voriconazole.…”

Section: Discussionmentioning

confidence: 99%

<I>Paecilomyces lilacinus</I>: An Emerging Pathogen

Ramya

Baby²,

Geetha

2016

JHSR

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Paecilomyces is a common saprophyte found in air and food and is also associated with many types of human infections. Pneumonia due to Paecilomyces is rare and the clinical manifestations are unspecific. So distinguishing Paecilomyces infection clinically and prompt microbiological identification will help in adminiatering empirical antifungal therapy. We describe a case of atypical pneumonia by Paecilomyces lilacinus in a patient with interstitial lung disease and cor pulmonale. The female patient had breathlessness for over two years and was admitted to hospital as it worsened. Her X-ray revealed right and left ventricular failure. Sputum was sent for fungal bacterial culture and KOH for fungal elements. The Culture on Sabourouds Dextrose Agar grew purecolonies of Paecilomyces.

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Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences

Cited by 66 publications

References 15 publications

Species confirmation of fungal isolates by molecular analysis

Species confirmation of fungal isolates by molecular analysis

Disseminated Mycosis in Veiled Chameleons ( Chamaeleo calyptratus ) Caused by Chamaeleomyces granulomatis , a New Fungus Related to Paecilomyces viridis

<I>Paecilomyces lilacinus</I>: An Emerging Pathogen

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