Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus)

Abstract

Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.

Microsatellite DNA markers applied to detection of multiple paternity in Caiman latirostris in Santa Fe, Argentina

“…Gels were stained with ethidium bromide and analyzed under UV light. We assumed amplification was successful when we obtained bands with estimated molecular weights within the range established by Zucoloto et al (2006). Products were analyzed by electrophoresis on 10% polyacrylamide gels at 2,200 V and 75 W and stained with AgNO 3 solution (Bassam et al,'91).…”

“…More recently, Villela (2004) recorded high values of genetic diversity and heterozygosity in C. latirostris using microsatellite markers specifically developed for this species Zucoloto, 2003). These broad-snouted caiman primers were also successfully applied to amplification of DNA samples of Paleosuchus palpebrosus, C. crocodylus and C. yacare (Zucoloto et al, 2006). This study aims to utilize microsatellite markers in order to understand the underlying genetic diversity and mating structure of C. latirostris in Santa Fe, Argentina, by following similar work conducted on other crocodilian species.…”

Assessment of microsatellites in estimating inter- and intraspecific variation among Neotropical Crocodylus species

“…Fortunately, a number of microsatellite loci are conserved enough across species to be useful in interspecific and intraspecific marker comparisons (Glenn et al, 1998). Successful cross-species amplification of microsatellites has been reported from groups such as mammals (Williamson et al, 2002;Gunn et al, 2005), birds (Primmer et al, 1996(Primmer et al, , 2005, insects (Wilson et al, 2004), fish (Scribner et al, 1996;King et al, 2001), snakes (King, 2009;Vandewege et al, 2012), and crocodilians (Dever and Densmore III, 2001;Zucoloto et al, 2006;Weaver et al, 2008;Milián-Garcia et al, 2011;Rodriguez et al, 2008Rodriguez et al, , 2011.…”

Culex quinquefasciatus (Diptera: Culicidae): aspectos da manipulação genética e estudos populacionais utilizando marcadores microssatélites

“…Por exemplo: o motivo mais abundante na maioria dos insetos é AC, mas em abelhas e vespas o motivo mais abundante é AG (Estoup et al 1993;Thoren et al 1995;Zucoloto et al 2006). Esta disparidade encontrada em taxa superiores não pode ser explicada somente pela diferença de composição entre genomas (Field & Wills 1996;Bachtrog et al 2000;Kruglyak et al 1998;Katti et al 2001).…”

“…Ao assumirmos que padrões de variação de microssatélites refletem processos de mutação em uma espécie, não é surpreendente que tais processos sejam distintos entre populações que foram separadas por milhões de anos (Zucoloto et al 2006). Loci microssatélites foram encontrados no genoma de todos os organismos em que foram procurados (Goldstein and Schloetterer 1999;Katti, et al 2001;Neff & Gross 2001;Zucoloto et al 2006). …”