“…However, Tup1 is involved in pleiotropic functions through interaction with specific DNA‐binding proteins for each functionally related set of genes (reviewed in Malave & Dent, 2006), while the Mig1‐dependent regulation is thought to be more specific, with a limited set of the target genes that include those repressed by glucose (Murad et al , 2001). Therefore, our results are in line with the previous observation that HpTup1 is not required for glucose repression of peroxisomal enzymes (Oliveira et al , 2003), and suggest that, at least for alcohol oxidase, operation of the classical S. cerevisiae pathway with Mig1/2‐mediated Tup1‐Ssn6 binding to the repressible promoter is unlikely. However, the redundant function of these repressors cannot be excluded, and the effect of combining all three mutations in one strain would be interesting to examine.…”