Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

Yaish, Mahmoud W.; Vega, M. Pérez de la

doi:10.1590/s1415-47572003000300019

Cited by 29 publications

(16 citation statements)

References 24 publications

(22 reference statements)

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“…In recent years, microsatellites for common beans have been developed from published sequences (Yu et al 1999;Blair et al 2003;Masi et al 2003;Guerra-Sanz 2004) and from microsatellite-enriched libraries (Métais et al 2002;Blair et al 2003;Gaitán-Solis et al 2002;Yaish et al 2003). Recently, two major publications have added new data information to the Phaseolus database (Ramírez et al 2005;Melotto et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers

Benchimol¹,

Campos

Carbonell³

et al. 2007

Genet Resour Crop Evol

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A common bean genomic library was constructed using the 'IAC-UNA' variety enriched for (CT) and (GT) for microsatellite motifs. From 1,209 sequenced clones, 714 showed microsatellites distributed over 471 simple and 243 compound motifs. GA/CT and GT/CA were the most frequent motifs found among these sequences. A total of 123 microsatellites has been characterized. Out of these, 87 were polymorphic (73.7%), 33 monomorphic (26.8%), and 3 (2.4%) did not amplify at all. In a sample of 20 common bean materials selected from the Agronomic Institute Germplasm Bank, the number of alleles per locus varied 2-9, with an average of 2.82. The polymorphic information content (PIC) of each marker varied from 0.05 to 0.83, with a 0.45 average value. Cluster and principal coordinate analysis of the microsatellite data were consistent with the original assignment of the germplasm accessions into the Andean and Mesoamerican gene pools of common bean. Low polymorphism levels detected could be associated with the domestication process. These microsatellites could be a valuable resource for the bean community because of their use as new markers for genetic studies.

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Section: Introductionmentioning

confidence: 99%

Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers

Benchimol¹,

Campos

Carbonell³

et al. 2007

Genet Resour Crop Evol

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“…They are also more widely distributed in the bean genome (Freyre et al 1998;Blair et al 2003). In common bean, around 400 microsatellite markers have been developed and mapped (Yu et al 2000;Gaitán-Solís et al 2002;Blair et al 2003;Masi et al 2003;Yaish and Pérez de la Vega 2003;GuerraSanz 2004;Caixeta et al 2005;Buso et al 2006). However, population studies with microsatellites in common bean so far have been performed only in a small number of landraces or breeding lines or they have focused on certain geographic regions (Métais et al 2002;Blair et al 2006;Díaz and Blair 2006).…”

Section: Introductionmentioning

confidence: 99%

Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae)

2009

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Domesticated materials with well-known wild relatives provide an experimental system to reveal how human selection during cultivation affects genetic composition and adaptation to novel environments. In this paper, our goal was to elucidate how two geographically distinct domestication events modified the structure and level of genetic diversity in common bean. Specifically, we analyzed the genome-wide genetic composition at 26, mostly unlinked microsatellite loci in 349 accessions of wild and domesticated common bean from the Andean and Mesoamerican gene pools. Using a model-based approach, implemented in the software STRUCTURE, we identified nine wild or domesticated populations in common bean, including four of Andean and four of Mesoamerican origins. The ninth population was the putative wild ancestor of the species, which was classified as a Mesoamerican population. A neighbor-joining analysis and a principal coordinate analysis confirmed genetic relationships among accessions and populations observed with the STRUC-TURE analysis. Geographic and genetic distances in wild populations were congruent with the exception of a few putative hybrids identified in this study, suggesting a predominant effect of isolation by distance. Domesticated common bean populations possessed lower genetic diversity, higher F ST , and generally higher linkage disequilibrium (LD) than wild populations in both gene pools; their geographic distributions were less correlated with genetic distance, probably reflecting seed-based gene flow after domestication. The LD was reduced when analyzed in separate Andean and Mesoamerican germplasm samples. The Andean domesticated race Nueva Granada had the highest F ST value and widest geographic distribution compared to other domesticated races, suggesting a very recent origin or a selection event, presumably associated with a determinate growth habit, which predominates in this race.

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“…The establishment in the glasshouse was aimed at obtaining young leaves for DNA extraction (Yaish and Vega 2003) and other samples for laboratory-based seed-borne disease detection tests. No phytosanitary observations were carried out in the glasshouse.…”

Section: Bean Varietiesmentioning

confidence: 99%

Seed-Health Assessment of Different Bean Seed Grades and Sources from Ethiopia Using Molecular Tools

Claude¹,

Dickinson

Mayes

et al. 2009

Journal of New Seeds

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Local seed systems, which supply about 99% of bean seeds in the east and central Africa regions, are progressively being supported as an alternative to facilitate the accessibility of improved bean varieties to farmers. However, these systems have been rightly or wrongly criticized for supplying substandard quality seeds. This study was carried out to assess the seed health of different seed grades from different sources of two popular Ethiopian bean varieties. Polymerase chain reaction (PCR) and terminal restriction fragment length polymorphism (T-RFLP) were used to detect fungal and bacterial pathogens of the major bean seed-borne diseases. The ELISA test was used to assess the status of the bean common mosaic virus (BCMV). No fungal or bacterial pathogens were detected in the seeds. However, BCMV was detected in almost all sources except seed from one untrained farmer and one grain merchant. 294 R. J. Claude et al.The study findings provide evidence about the capacity of the local seed system to produce and supply seed of an acceptable quality.

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Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

Cited by 29 publications

References 24 publications

Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers

Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers

Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae)

Seed-Health Assessment of Different Bean Seed Grades and Sources from Ethiopia Using Molecular Tools

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