AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)

Abstract

The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F 2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown to be associated with water-stress tolerance in segregating F 2 progeny of the barley cross used in a previous study. AFLP analysis was then performed on these DNA pools, using 40 primer pairs to detect AFLP fragments that are present/absent, respectively, in the two pools and their parental lines. One separate AFLP fragment, which was present in the tolerant parent and in the tolerant bulk, but absent in the sensitive parent and in the sensitive bulk, was identified. Polymorphism of the AFLP marker was tested among tolerant and sensitive F 2 individuals. The presence of this marker that is associated with water-stress tolerance will greatly enhance selection for paraquat and water-stress tolerant genotypes in future breeding programs.

Fine Mapping QTL for Drought Resistance Traits in Rice (Oryza sativa L.) Using Bulk Segregant Analysis

“…Though the potential of BSA has been demonstrated to tag genes controlling qualitative traits, the method could now be extended for analysis of complex traits such as drought resistance. Markers linked to drought-resistance traits have been identified using BSA in barley [26], wheat [27] and maize [28]. Markers linked to yield [29] and yield under drought stress in rice [24] have also been identified using BSA.…”

Identification of QTLs for pod and kernel traits in cultivated peanut by bulked segregant analysis

“…Molecular marker analysis is useful for identification of genomic segments contributing to the genetic variance of a trait, and selection of superior genotypes. Marker analysis provides accurate genotypic information, which would be unavailable otherwise, giving a precision lacking with phenotypic measurements due to environmental interaction and experimental error (Altinkut et al 2003). Molecular markers are sought for early and reliable selection of desired pod and kernel genotypes in breeding programs (Samizadeh et al 2003).…”

Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

“…BSA was reported by Michelmore et al (1991) to identify RAPD markers tightly linked to genes for resistance to lettuce downy mildew. In recent years, some markers tightly linked to genes were found by using BSA method as such or with slite modificatiom (Altinkue et al, 2003; Govindaraj et al, 2005; Kotresh et al, 2006; Podlich et al, 2004; Mackay and Caligari, 2000; Xu et al, 1995; Zheng et al, 2002). BSA analysis involves screening for differences between pooled DNA samples derived from a segregating population that originated from a single cross, which improves the efficiency of primer screening (Michelmore et al, 1991).…”