Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae)

Chippari-Gomes, Adriana Regina; Leitão, M. A. B.; Paula-Silva, Maria de Nazaré; Mesquita-Saad, L.S.B; Almeida-Val, Vera Maria Fonseca de

doi:10.1590/s1415-47572003000100005

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…Variation of the dissolved oxygen concentrations (mg L −1 ) during 24 h at different depths (cm) in the Catalão Lake (Amazonas‐Brazil) at October 2000 and March 2001. Note that the oxygen concentration varies depending on the period of the year, time of day, and measurement depth (Chippari‐Gomes et al, 2003, unpublished data) [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: The Amazonian Environment: Shaping the Evolution Of Hypoxia‐tolerance In Amazonian Fish Speciesmentioning

confidence: 99%

Ecological adaptations of Amazonian fishes acquired during evolution under environmental variations in dissolved oxygen: A review of responses to hypoxia in fishes, featuring the hypoxia‐tolerantAstronotusspp.

Braz-Mota

Almeida-Val

2021

J Exp Zool Pt A

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The Amazon Basin presents a dynamic regime of dissolved oxygen (DO) oscillations, which varies among habitats within the basin, including spatially, daily, and seasonally. Fish species inhabiting these environments have developed many physiological adaptations to deal with the frequent and periodic events of low (hypoxia), or no (anoxia) DO in the water. Cichlid fishes, especially the genus Astronotus (A. ocellatus and A. crassipinnis), are hypoxic‐tolerant species that can survive in very low DO levels for long periods, while adults often inhabit places where DO is close to zero. The present review will focus on some metabolic adjustments that Amazonian fish use in response to hypoxic conditions, which include many strategies from behavioral, morphological, physiological, and biochemical strategies. These strategies include ASR (aerial surface respiration), lip expansion, branchial tissue remodeling, increases in glycolytic metabolism with the increase of blood glucose levels, and increases in anaerobic metabolism with increases of plasma lactate levels. Other groups over evolutionary time developed obligate aerial respiration with changes in pharyngeal and swim bladder vascularization as well as the development of a true lung. However, most species are water‐breathing species, such as A. ocellatus and A. crassipinnis, which are detailed in this study because they are used as hypoxia‐tolerant model fish. Herein, we draw together the literature data of the physiological mechanisms by which these species decrease aerobic metabolism and increase anaerobic metabolism to survive hypoxia. This is the first attempt to synthesize the physiological mechanisms of the hypoxia‐tolerant Astronotus species.

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Section: The Amazonian Environment: Shaping the Evolution Of Hypoxia‐tolerance In Amazonian Fish Speciesmentioning

confidence: 99%

Ecological adaptations of Amazonian fishes acquired during evolution under environmental variations in dissolved oxygen: A review of responses to hypoxia in fishes, featuring the hypoxia‐tolerantAstronotusspp.

Braz-Mota

Almeida-Val

2021

J Exp Zool Pt A

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“…또한 호기적 조건에서 피루브산이 아세틸 CoA로 전환되고 시트르산합성 효소(EC 4.1.3.7, citrate synthase, CS)에 의해 시트르산을 생성 하므로 CS는 산화적 대사의 지표로 사용된다 [3,22]. 따라서 조 직에서 혐기적 대사와 호기적 대사의 정도는 LDH/CS로 연구 되었다 [8,36].…”

Section: 서 론unclassified

Purification and Characterization of Lactate Dehydrogenase Isozymes in Channa argus

Park¹,

Yum²

2010

Journal of Life Science

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The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Channa argus were purified and characterized by biochemical, immunochemical and kinetic methods. The activity of LDH in skeletal muscle was the highest at 380.4 units and those in heart, eye and brain tissues were 13.4, 3,5 and 5.4 units, respectively. Citrate synthase (EC 4.1.3.7, CS) activity in heart tissue was the highest at 20.7 units. LDH/CS in skeletal muscle, heart, eye and brain tissues were 172.9, 0.6, 0.32 and 0.47. Protein concentration in skeletal muscle tissue was 14.7 mg/g and specific activities of LDH in skeletal muscle, heart, eye and brain tissues were 25.88, 0.79, 0.31 and 1.38 units/mg, respectively. Therefore, skeletal muscle tissue was anaerobic and heart tissue was aerobic. The LDH isozymes in tissues were identified by polyacrylamide gel electrophoresis, immunoprecipitation and Western blot with antiserum against A4, B4, and eye-specific C4. LDH A4, A3B, A2B2. AB3 and B4 isozymes were detected in every tissue, C4, AC3, A2C2 and A3C were detected in eye tissue, and A3C was found in brain tissue. LDH A4, A3B, A2B2, AB3, B4, eye-specific C4 isozymes were purified by affinity chromatography and Preparative PAGE Cells. The LDH A4 isozyme was purified in the fraction from elution with NAD + containing buffer of affinity chromatography. Eye-specific C4 isozyme was eluted right after A4, after which B4 isozyme was eluted with plain buffer. As a result, one part of molecular structures in A4, B4 and eye-specific C4 were similar, but were different from each other in B4 and C4. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The activity of LDH A4, A2B2, B4, and eye-specific C4 isozymes remained at 39.98, 21.28, 19.67 and 16.87% as a result of the inhibition by 10 mM of pyruvate, so the degree of inhibition was very high. The Km PYR values were 0.17, 0.27 and 0.133 mM in A4, B4 and eye-specific C4 isozymes, respectively. The optimum pH of LDH A4, B4, eye-specific C4, A2B2, A3B, and AB3 were pH 6.5, pH 8.5, pH 5.5, pH 6.0-6.5, pH 5.0 and pH 7.5. The A4 and heterotetramer isozymes stabilized a broad range of pH. Especially, LDH activities in skeletal muscle tissue were high, resulting in a high degree of muscle activity.LDH metabolism in eye tissue seems to be converted faster from pyruvate to lactate by eye-specific C4 isozyme as eye-specific C4 have the highest affinity for pyruvate, and right after the conversion, oxidation of lactate was induced by A4 isozyme. It was found that expression of Ldh-C, affinity to substrate and reaction time of C4 isozyme were different according to the ecological environmental and feeding capturing patterns.

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“…Temperature and oxygen availability are among predominant environmental determinants of evolutionary as well as physiological adaptation in teleost fishes (Hochachka 1980;Das & Ratha 1996;Pierce & Crawford 1997;Somero 2004). LDH activity or LDH isoenzymes are one of the best indicators of switch-over to anaerobic metabolism by which teleosts survive periods of oxygen unavailability (Chippari-Gomes et al 2003). However, mostly the behavioral air-breathers have been the subject of stress studies, while information is scanty on air-breathing teleosts which possess air-breathing organs (ABOs).…”

Section: Introductionmentioning

confidence: 99%

Functional and adaptive significance of differentially expressed lactate dehydrogenase isoenzymes in tissues of four obligatory air-breathing Channa species

Ahmad

2009

Biologia

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This study investigates the differential expression of lactate dehydrogenase (LDH) isoenzymes in the genus Channa using PAGE. With the help of obligate air-breathing, all of the selected species can sustain water deprivation to varying degrees. In subunit composition and higher electrophoretic mobility of LDH-A4, the profiles of channid species were similar to other teleosts documented in the literature. However, inter-and intra-species differences, with particular reference to aerobic/anaerobic metabolic options, existed. Whereas glycolysis in Channa punctata appears to depend largely on aerobic LDH-B and partly on anaerobic LDH-A, metabolism in C. gachua, C. striata and C. marulius depends exclusively on the activity of anaerobic LDH-A. Expression of the third locus Ldh-C was recorded in the eyes of C. marulius, in addition to C. gachua. Heat inactivation experiments reveal species differences between LDH isoenzymes and a general order of the relative stabilities: LDH-C > LDH-B > LDH-A. Metabolic and evolutionary implications of the findings have also been discussed.

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Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae)

Cited by 7 publications

References 18 publications

Ecological adaptations of Amazonian fishes acquired during evolution under environmental variations in dissolved oxygen: A review of responses to hypoxia in fishes, featuring the hypoxia‐tolerantAstronotusspp.

Ecological adaptations of Amazonian fishes acquired during evolution under environmental variations in dissolved oxygen: A review of responses to hypoxia in fishes, featuring the hypoxia‐tolerantAstronotusspp.

Purification and Characterization of Lactate Dehydrogenase Isozymes in Channa argus

Functional and adaptive significance of differentially expressed lactate dehydrogenase isoenzymes in tissues of four obligatory air-breathing Channa species

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