Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)

Moysés, Cinthia Bachir; Almeida-Toledo, Lurdes Foresti de

doi:10.1590/s1415-47572002000400008

Cited by 27 publications

(17 citation statements)

References 21 publications

(17 reference statements)

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“…As already mentioned, Garutti and Britski (2000) have noted that the morphology of this lambari is not uniform from population to population, probably because these fish inhabit a wide variety of microenvironments, and thus it is to be expected that the populations found at different points along a river should display genetic structuring. Moysés and Almeida-Toledo (2002) analysed restriction-fragment length polymorphism of mitochondrial DNA among five freshwater fish of the genus Astyanax from Upper Paraná and São Francisco hydrographic basins. In this study, nine different mtDNA haplotypes were detected in A. altiparanae, eight of them population-specific.…”

Section: Analysis Of the Genetic Structure Of Astyanax Altiparanae Inmentioning

confidence: 99%

Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil

et al. 2004

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In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows that the low Paranapanema is highly differentiated from the middle (0.2813) and upper (0.2912) Paranapanema, while the differentiation between the last two is moderate (0.0895). The data obtained in the present work suggest that recolonization and conservation studies should not be focused on the species A. altiparanae as such, but on the conservation units, because they are the genetically differentiated populations.

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Section: Analysis Of the Genetic Structure Of Astyanax Altiparanae Inmentioning

confidence: 99%

Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil

et al. 2004

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“…Protein electrophoresis on starch gel was first used more than 45 years ago to identify species (Manwell and Baker 1963). Since then, many studies have been published using a diverse assemblage of molecular approaches and markers to identify species, such as allozymes (Aron and Sole-Cava 1991;Gusmão et al 2000), restriction fragment length polymorphism (Moysés and Almeida-Toledo 2002), DNA arrays (Hajibabei et al 2007), single nucleotide polymorphism (Shaffer and Thonsom 2007), multiplex PCR (Mendonça et al 2009), DNA sequences (Pook and McEwing 2005;Lemer et al 2007), and many others.…”

Section: Introductionmentioning

confidence: 99%

DNA barcodes discriminate freshwater fishes from the Paraíba do Sul River Basin, São Paulo, Brazil

et al. 2011

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Background and aims: Considering the promising use of DNA barcoding for species identification, the importance of the freshwater fish fauna of the Paraíba do Sul River Basin, and its advanced stage of degradation, the present study evaluated the effectiveness of DNA barcoding to identify the fish species in this basin. Materials and methods: A total of 295 specimens representing 58 species belonging to 40 genera, 17 families, and 5 orders were sequenced. Results: The DNA barcodes discriminated all species analyzed without ambiguity. The results showed a pronounced difference between conspecific and congeneric pair-wise sequence comparisons, demonstrating the existence of a "barcode gap" for the species analyzed. The nearest-neighbor distance analysis showed only three cases with Kimura two-parameter values lower than a 2% divergence threshold. However, the patterns of divergence observed in each case remained sufficient to discriminate each species, revealing the accuracy of DNA barcoding even cases with relatively low genetic divergence. At the other extreme, three species displayed high genetic sequence divergence among conspecifics. For two cases, Characidium alipioi and Geophagus proximus, barcoding proved effective at flagging possible new species. For another case, Astyanax bimaculatus, the use of DNA barcoding of the comparison of shared freshwater fish fauna between different basins revealed itself as highly useful in disclosing that the previously identified A. bimaculatus "cluster A" probably represents the species Astyanax altiparanae. Conclusion: The present study is among the first to assess the efficiency of barcoding for the Brazilian freshwater fishes. The results demonstrate the utility of barcoding to identify the fauna from this basin, contribute to an enhanced understanding of the differentiation among species, and to help flag the presence of overlooked species.

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“…Other workers have also used molecular markers to compare the genetic variability of A. altiparanae populations from other Brazilian rivers. Moysés and Almeida-Toledo (2002) studied the genetic variability of the mitochondrial DNA (mtDNA) of Astyanax lacustris from the São Francisco River basin and A. altiparanae from the PR basin using the restriction fragment length polymorphism (RFLP) method and found differences between these two populations. used random amplified polymorphic DNA (RAPD) and mtDNA markers to demonstrate strong genetic similarities between A. altiparanae populations from the Keller and Pirapó rivers in the PR basin and the Iguaçu River.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

Peres

Vasconcelos²,

Renesto

2005

Genet. Mol. Biol.

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Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase -NADP + , 1.1.1.42 (IDH), L-Lactate dehydrogenase, 1.1.1.27 (LDH), Malate dehydrogenase, 1.1.1.37 (MDH), Malate dehydrogenase -NADP + , 1.1.1.40 (MDHP), Phosphoglucomutase, 5.4.2.2 (PGM), and Superoxide dismutase, 1.15.1.1 (SOD). The proportion of polymorphic loci were estimated as 52.38% in the PR population and 38.10% in the RF population. Expected estimated heterozygosities were 0.1518 ± 0.0493 for the PR population and 0.0905 ± 0.0464 for the RF population. The A. altiparanae heterozygosity data were similar to previous estimates for other PR basin characid species. Allele frequencies were significantly different between the PR and RF populations in respect to some loci (Acp-1, G3pdh-1, Gpi-A, Iddh-1, Mdhp-1 and Mdhp-2). Wright's statistics for all loci were estimated as F is = 0.3919, F it = 0.4804 and F st = 0.1455. Our results show that the A. altiparanae populations studied are genetically different and have a high degree of genetic variability.

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Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)

Cited by 27 publications

References 21 publications

Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil

Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil

DNA barcodes discriminate freshwater fishes from the Paraíba do Sul River Basin, São Paulo, Brazil

Genetic variability in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil

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