Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos

Marins, Luís Fernando; Iyengar, Arati; Maclean, Norman; Levy, J.A.; Sohm, Frédéric

doi:10.1590/s1415-47572002000300007

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…In chicken, two SNPs in STAT5B were found to be associated with early body weight and egg weight, suggesting a promising marker for use in marker‐assisted selection in this species (Sadeghi et al, 2012 ). In Nile tilapia, the overexpression of STAT5B can neutralize the effects of GH overexpression, suggesting the partial role of STAT5B on the deleterious effects of GH overexpression observed in genetically selected fish (Marins et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Increased accuracy of genomic predictions for growth under chronic thermal stress in rainbow trout by prioritizing variants from GWAS using imputed sequence data

Yoshida

Yáñez

2021

Evolutionary Applications

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Through imputation of genotypes, genome‐wide association study (GWAS) and genomic prediction (GP) using whole‐genome sequencing (WGS) data are cost‐efficient and feasible in aquaculture breeding schemes. The objective was to dissect the genetic architecture of growth traits under chronic heat stress in rainbow trout ( Oncorhynchus mykiss ) and to assess the accuracy of GP based on imputed WGS and different preselected single nucleotide polymorphism (SNP) arrays. A total of 192 and 764 fish challenged to a heat stress experiment for 62 days were genotyped using a customized 1 K and 26 K SNP panels, respectively, and then, genotype imputation was performed from a low‐density chip to WGS using 102 parents (36 males and 66 females) as the reference population. Imputed WGS data were used to perform GWAS and test GP accuracy under different preselected SNP scenarios. Heritability was estimated for body weight (BW), body length (BL) and average daily gain (ADG). Estimates using imputed WGS data ranged from 0.33 ± 0.05 to 0.55 ± 0.05 for growth traits under chronic heat stress. GWAS revealed that the top five cumulatively SNPs explained a maximum of 0.94%, 0.86% and 0.51% of genetic variance for BW, BL and ADG, respectively. Some important functional candidate genes associated with growth‐related traits were found among the most important SNPs, including signal transducer and activator of transcription 5B and 3 ( STAT5B and STAT3 , respectively) and cytokine‐inducible SH2‐containing protein ( CISH ). WGS data resulted in a slight increase in prediction accuracy compared with pedigree‐based method, whereas preselected SNPs based on the top GWAS hits improved prediction accuracies, with values ranging from 1.2 to 13.3%. Our results support the evidence of the polygenic nature of growth traits when measured under heat stress. The accuracies of GP can be improved using preselected variants from GWAS, and the use of WGS marginally increases prediction accuracy.

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Section: Discussionmentioning

confidence: 99%

Increased accuracy of genomic predictions for growth under chronic thermal stress in rainbow trout by prioritizing variants from GWAS using imputed sequence data

Yoshida

Yáñez

2021

Evolutionary Applications

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“…The F0104 lineage was produced by the co-injection of transgenes cbA/ msGH and cbA/GFP. Both transgenes include the carp (Cyprinus carpio) b-actin promoter and either marine silverside (Odontesthes argentinensis) GH cDNA (Marins et al 2002) or the green fluorescent protein (GFP) gene. GFP was used as a marker for successful gene transfer, allowing identification through fluorescence analysis (excitation = 485 nm; emission = 520 nm).…”

Section: Transgenic Fishmentioning

confidence: 99%

GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model

Kuradomi

Figueiredo

Lanes³

et al. 2010

Transgenic Res

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The aim of the present study was to analyse the morphology of white skeletal muscle in males and females from the GH-transgenic zebrafish (Danio rerio) lineage F0104, comparing the expression of genes related to the somatotrophic axis and myogenesis. Histological analysis demonstrated that transgenic fish presented enhanced muscle hypertrophy when compared to non-transgenic fish, with transgenic females being more hypertrophic than transgenic males. The expression of genes related to muscle growth revealed that transgenic hypertrophy is independent from local induction of insulin-like growth factor 1 gene (igf1). In addition, transgenic males exhibited significant induction of myogenin gene (myog) expression, indicating that myog may mediate hypertrophic growth in zebrafish males overexpressing GH. Induction of the α-actin gene (acta1) in males, independently from transgenesis, also was observed. There were no significant differences in total protein content from the muscle. Our results show that muscle hypertrophy is independent from muscle igf1, and is likely to be a direct effect of excess circulating GH and/or IGF1 in this transgenic zebrafish lineage.

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“…Freshly fertilized eggs were collected for microinjection and transgenic zebrafish produced using two transgenes containing the carp (Cyprinus carpio) β-actin promoter driving the expression of either the A. victoria GFP gene (pcβA/GFP plasmid) or the marine silverside fish (Odonthestes argentinensis) growth hormone (msGH) cDNA (pcβA/msGH plasmid). The pcβA/GFP plasmid was kindly provided by Dr. Suzanne Brooks (University of Southampton, UK) and was used to produce the pcβA/ msGH plasmid by replacing the GFP gene with the msGH cDNA (Marins et al, 2002). Both plasmids were linearized with the Spe I restriction enzyme and co-injected at a 1:1 molar ratio into one-cell embryos using a total DNA concentration of 35 ng μL -1 .…”

Section: Production Of Transgenic Fishmentioning

confidence: 99%

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy

et al. 2007

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In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp β-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes argentinensis. The methodology applied allowed a rapid identification of G 0 transgenic fish and also detected which fish were transmitting transgenes to the next generation. This strategy also allowed inferences to be made about genomic transgene integration events in the six lineages produced and allowed the identification of one lineage transmitting both transgenes linked on the same chromosome. These results represent a significant advance in the reduction of the effort invested in producing a stable genetically modified fish lineage.

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Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos

Cited by 9 publications

References 30 publications

Increased accuracy of genomic predictions for growth under chronic thermal stress in rainbow trout by prioritizing variants from GWAS using imputed sequence data

Increased accuracy of genomic predictions for growth under chronic thermal stress in rainbow trout by prioritizing variants from GWAS using imputed sequence data

GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy

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