Genetic instability of sugarcane plants derived from meristem cultures

Zucchi, Maria Imaculada; Arizono, H.; Morais, Vicente Alberto; Fungaro, Maria Helena Pelegrinelli; Vieira, Maria Lúcia Carneiro

doi:10.1590/s1415-47572002000100017

Cited by 57 publications

(53 citation statements)

References 30 publications

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“…Maritano et al (2010) suggest a distance value of 0.05 to consider that the clones obtained by in vitro culture are genetically identical, so according to the results, obtained calluses are not clones since they showed genetic variability. Zucchi et al (2002) reported polymorphism ranges of 3.06% to 5.1% between the mother plant and explants derived from sub-crops in sugarcane, polymorphism occurred in all sub-cultures and did not increase with the number thereof; as in our work, higher level of variation between the mother plant and established explants in vitro was shown. The process of in vitro culture is a stressing factor for the genome of the plant; during the cultivation in vitro, the plant suffers cell dedifferentiation, mainly in the first generation, in the next generations or sub-cultures, the level of polymorphism decreases, due to non-predictable processes (Zucchi et al 2002).…”

Section: Combinación De Iniciadoressupporting

confidence: 72%

“…Maritano et al (2010) sugieren un valor de distancia de 0.05 para considerar que los clones obtenidos por cultivo in vitro son genéticamente idénticos, por lo que de acuerdo con los resultados, los callos obtenidos no son clones ya que presentaron variabilidad genética. Zucchi et al (2002) reportaron intervalos de polimorfismo de 3.06% a 5.1% entre la planta madre y los explantes derivados de los sub-cultivos en caña de azúcar, los polimorfismos se presentaron en todos los sub-cultivos y no se incrementaron con el número de los mismos; al igual que en nuestro trabajo se mostró mayor nivel de variación entre la planta madre y los explantes establecidos in vitro. El proceso de cultivo in vitro es un factor estresante para el genoma de la planta, durante el cultivo in vitro la planta sufre des-diferenciación celular…”

Section: Combinación De Iniciadoresunclassified

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Variabilidad genética de cultivo in vitro de aguacate raza mexicana

Cerda-Hurtado

Ojeda-Zacarías

Iracheta-Donjuan

et al. 2018

Remexca

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ResumenExplantes foliares de Persea americana Mill. var. drymifolia, se establecieron en medio DCR (Gupta y Durzan, 1985) suplementado con vitaminas y reguladores del crecimiento para producir callos, dos sub cultivos se realizaron en intervalos de 30 a 45 días entre ellos. Con el fin de determinar la variabilidad genética se generaron 341 marcadores AFLP (amplified fragment lenght polymorphism) polimórficos con 10 combinaciones de iniciadores, se analizaron 94 muestras: la planta madre, 31 callos, 31 callos del primer sub cultivo y 31 callos del segundo sub cultivo. Se determinó el contenido de información polimórfica (PIC) por combinación de iniciadores y se calculó la distancia genética entre muestras para hacer el análisis de agrupamiento. Se obtuvo la diversidad genética entre la planta madre, callos y callos de los dos sub cultivos y se realizó ei análisis de varianza molecular. Los valores PIC indicaron que las combinaciones de iniciadores fueron adecuadas para estimar la variabilidad genética de los callos. En el análisis de agrupamiento, la planta madre mostró distancias de 0.63 a 0.7 con respecto a sus callos, distancias de 0.78-0.99 se obtuvieron entre los callos y los callos de los dos sub cultivos. La diversidad genética presentó diferencias significativas, el análisis de varianza molecular demostró variabilidad genética entre AbstractLeaf explants of Persea americana Mill. var. drymifolia were established on DCR medium (Gupta and Durzan, 1985) supplemented with vitamins and growth regulators to produce callus, two sub cultures were made in intervals of 30 to 45 days between them. In order to determine the genetic variability 341 AFLP (amplified fragment lenght polymorphism) polymorphism with 10 primer combinations were generated, 94 samples were analyzed: the mother plant, 31 calluses, 31 calluses from the first sub culture and 31 calluses from the second sub culture. Polymorphic information content (PIC) was determined by primer combination and the genetic distance between samples was calculated for the cluster analysis. Genetic diversity between the mother plant, calluses and calluses of the two sub cultures were obtained and the analysis of molecular variance was performed. The PIC values indicated that the primer combinations were adequate to estimate the genetic variability of calluses. In the cluster analysis, the mother plant showed distances of 0.63 to 0.7 in regard to their calluses, distances of 0.78 -0.99 were obtained between calluses and calluses of the two sub cultures. Genetic diversity showed significant differences, analysis of molecular variance demonstrated genetic variability between the mother plant, calluses and calluses of the two sub cultures IntroducciónEn plantas el cultivo in vitro es una alternativa para la conservación ex situ, el intercambio y conservación de germoplasma y la propagación clonal; la selección del explante depende de los recursos y objetivos finales de la técnica. Diversos explantes se han utilizado para obtener tipos diferentes de morfogénesis en agu...

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Section: Combinación De Iniciadoressupporting

confidence: 72%

Section: Combinación De Iniciadoresunclassified

Variabilidad genética de cultivo in vitro de aguacate raza mexicana

Cerda-Hurtado

Ojeda-Zacarías

Iracheta-Donjuan

et al. 2018

Remexca

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“…Entretanto, recentemente, ROSENBERG et al (2010 demonstraram que clones de batata da cultivar 'Reet', originados de ápices caulinares, apresentaram variação somaclonal para características fenotípicas como rendimento. Em cana-de-açúcar, identificaram-se somaclones em genótipos originados de ápices caulinares (ZUCCHI et al, 2002).…”

Section: Resultsunclassified

“…A identificação de alterações em características morfológicas em plantas de batata provenientes da cultura de calos foi estudada, há bastante tempo, por alguns autores (RAMULU et al, 1983;BORDALLO et al, 2004). Existem evidências que, sob determinadas condições, plantas de cana-de-açúcar e de batata, oriundas de organogênese direta também apresentem variação somaclonal (ZUCCHI et al, 2002;ROSENBERG et al, 2010;SANTIAGO et al, 2012).…”

Section: Introductionunclassified

Somaclones em batata identificados pelos descritores mínimos de broto

2015

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“…It was reported that variation may be resulted from the pre-existing variation of the explants (Wakasa 1979;Nwauzoma & Jaja 2013), the et al long period of the culture (Koornneef 1991;Masoud & Hamta 2008), the high level of plant growth regulator (Bairu 2006), the frequent et al subculture (Eeuwens 2002), the genotype et al dependence (Zucchi . 2002), activation of et al transposable elements (Bairu 2011), and et al hypo-or hypermethylation of DNA (Abdellatif et al 2012).…”

Section: Introductionmentioning

confidence: 99%

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

Roostika

Khumaida²,

Ardie³

2016

BIOTROPIA

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Plant off-type becomes a concern in pineapple micropropagation. Reliable methods are needed to detect and to reduce plant off-type. This research was conducted to confirm the occurrence of somaclonal variation during micropropagation of pineapple clone Simadu. The culture period (long-and short-period) and the regeneration methods (direct organogenesis, indirect organogenesis and somatic embryogenesis) were studied to know their contribution in bringing out somaclonal variation. RAPD analysis using 10 primers was performed to confirm genetic variation. The result showed that RAPD assay could be applied as early detection of somaclonal variation of pineapple, where OPA primers were better to be used than OPJ primers. The phenotypic variation occurred in the four year-old pineapple plants and plantlets were due to genetic variation. It has proved that the long-period of cultures is the main contributor of somaclonal variation, while the regeneration method and plant growth regulator could also induce genetic variation. The new cultures showed higher level of similarity. Therefore, it needs a correct strategy to apply micropropagation method of pineapple to minimize plant off-type. It was recommended to avoid the use of long-period culture as mother stock and to apply direct organogenesis method for micropropagation rather than indirect organogenesis and somatic embryogenesis.

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Genetic instability of sugarcane plants derived from meristem cultures

Cited by 57 publications

References 30 publications

Variabilidad genética de cultivo in vitro de aguacate raza mexicana

Variabilidad genética de cultivo in vitro de aguacate raza mexicana

Somaclones em batata identificados pelos descritores mínimos de broto

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

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