Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia

Abstract

Chronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybridization), applying centromeric probes for chromosome 12 has made it possible to detect a higher percentage of trisomy 12 cases. The objective of the present study was to detect trisomy 12 by FISH (alpha satellite probe) in 13 patients with CLL whose karyotypes by G-banding were either normal or inadequate. Using this method trisomy 12 was detected in three patients in a percentage of positive cells varying from 55.5% to 79%, showing that FISH is a sensitive and highly specific method for trisomy detection and should be routinely performed when the karyotype is normal.<br>A leucemia linfocítica crônica (CLL) apresenta incidência variável de anomalias de cariótipo devido às dificuldades em se obter mitose para análise. Desde a introdução da citogenética molecular (FISH = hibridação in situ por fluorescência) usando sonda centromérica para o cromossomo 12 foi possível detectar uma maior porcentagem de casos com trissomia 12. O objetivo deste trabalho foi de detectar trissomia 12 por FISH (sonda alfa satélite) em 13 pacientes com CLL cujos cariótipos por banda G haviam sido normais ou sem resultado. Três pacientes apresentaram trissomia 12 por este método com uma porcentagem de células trissômicas variando de 55,5 a 79%, demonstrando que a FISH é um método sensível e altamente específico para detecção de trissomia 12

MgCl2‐supported catalyst containing mixed internal donors for propylene polymerization

“…Cytogenetic analysis using G‐banding was performed on metaphase chromosomes obtained from 200 peripheral blood lymphocytes and from 50 gonadal fibroblasts. FISH analysis was carried out, using Yp telomere probes, SRY (Vysis, Inc., Downers Grove, IL), and AneuVysion (X/Y/18) (Vysis, Inc.), in order to detect alpha‐satellite sequences in the centromeric regions of chromosomes X,Y, and 18, as described previously [Chauffaille et al, 2000].…”

Determination of the sexual phenotype in a child with 45,X/46,X,Idic(Yp) mosaicism: Importance of the relative proportion of the 45,X line in gonadal tissue

“…FISH analysis was carried out, using Yp telomere probes, SRY (Vysis, Inc., Downers Grove, IL), and AneuVysion (X/Y/18) (Vysis, Inc.), in order to detect alpha-satellite sequences in the centromeric regions of chromosomes X,Y, and 18, as described previously [Chauffaille et al, 2000].…”