Electrophoretic characterization of Aspergillus nidulans strains with chromosomal duplications

Queiroz, Marisa Vieira de; Pizzirani-Kleiner, Aline Aparecida; Azevedo, João Lúcio de

doi:10.1590/s1415-47572000000200009

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…Karyotype analysis, genome sizing and gene mapping of Paracoccidioides brasiliensis were well studied by PFGE techniques ( Cano et al., 1998; Feitosa et al., 2003). Queiroz et al. (2000) used PFGE to determine the electrophoretic karyotype of Aspergillus nidulans with chromosomal duplications, sizing and locating of the chromosomal duplication.…”

Section: Introductionmentioning

confidence: 99%

Studies on the regeneration‐restore and karyotype of protoplast in Metarhizium anisopliae var. majus

Shi

Miao

2005

J Applied Entomology

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The characteristics and regeneration-restore of protoplasts and its karyotype of an insect pathological fungus, Metarhizium anisopliae var. majus were studied. Among the protoplasts, 25.3% were without a nucleus, and 74.7% contained a nucleus. Among the nucleus protoplasts, 53.6% contained a single nucleus. The regeneration-restore of protoplasts was of three distinct shapes. Considering the frequency of regeneration and the growing speed of the colony, 0.7 mol/l glucose was the optimum as osmotic stabilizer of culture medium in the regeneration-restore of the protoplasts. The chromosomal DNA molecules of M. anisopliae var. majus have been separated into seven bands by pulsed-field gel electrophoreses. Using the Schizosaccharomyces pombe chromosomes as size standard, the size of chromosomal DNA was estimated to be 1.1-6.5 Mb and its karyotype exhibited polytypism among strains.

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Section: Introductionmentioning

confidence: 99%

Studies on the regeneration‐restore and karyotype of protoplast in Metarhizium anisopliae var. majus

Shi

Miao

2005

J Applied Entomology

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“…The buffer was replaced every 72 h and maintained in circulation at 10°C. Schizosaccharomyces pombe (Bio-Rad, USA) and Aspergillus nidulans (Queiroz et al, 2000) chromosomes were run in parallel as standard size markers. In both runs, 200 µM of thiourea was added to both the agarose gel and the electrophoresis buffer to ensure minimal DNA degradation (Fawley and Wilcox, 2002).…”

Section: Preparation Of Intact Chromosomal Dna and Pulsed-field Gel E...mentioning

confidence: 99%

Genetic variability and host specialization among brazilian populations of Ceratocystis fimbriata s.l.

Fernandes¹

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Ceratocystis fimbriata is one of the most important woody plant pathogens worldwide. The fungi colonize the vascular tissue, causing stains on the wood and death of branches or whole plants. Due the wide range of hosts, it is possible that adapted populations of C. fimbriata have been selected to specific hosts and that there is high genetic and physiological variability within the pathogen population. The knowledge of physiological factors that determine host range and host specificity are important aspects for disease management. Therefore, the aims of this study were: (i) determine the specializing for their hosts of 11 isolates on eight hosts; (ii) to investigate the karyotype of isolates of the C. fimbriata species complex from different host plants and geographical origins in Brazil and; (iii) assessed the variability genetic of karyotyped isolates using retrotransposon-microsatellite amplified polymorphism (REMAP). In addition, the identity of the isolates was confirmed conducting multilocus phylogeny using DNA sequences of mating type genes, TEF-1α, and β-tubulin. The analysis based on the C. fimbriata microsatellite (SSR) profiles showed a separation of isolates according to the host. Based on the results of inoculation of 11 isolates on eight hosts, a wide variation in aggressiveness was found. The isolates that were used in this study were more aggressive in their respective hosts. Some hosts, however, were susceptible to isolates from other hosts. Considering the length of xylem lesion caused by C. fimbriata isolates, F. carica was the most susceptible to the isolates tested, followed by M. indica. Only T. cacao and C. guianensis isolates proved to be specialized by their hosts. Polymorphism in chromosome number and size was found, indicating the existence of genomic differences among isolates and occurrence of chromosomal rearrangements in the species complex. The number of chromosomes varied from six to eight and the estimated minimum chromosome sizes were estimated to be between 2.7 to 6.0 Mbp. Small polymorphic chromosomes were observed in all isolates, raising the hypothesis that they could be supernumerary chromosomes. Because chromosomal variation can be caused by transposable elements (TEs), we also assessed the variability of the same isolates using retrotransposon-microsatellite amplified polymorphism (REMAP) molecular marker. REMAP analysis of karyotyped isolates revealed genetic variability and isolates from the same host tend to group in a same cluster.Keywords: Chromosomal rearrangements. Genomic architecture. PFGE. Ceratocystis wilt. Cross-inoculations. Physiological variability.

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Molecular Evolution of Aspergillus

Flores-Gallegos

Veana

Michel

et al. 2016

New and Future Developments in Microbial Biotechnology and Bioengineering

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Electrophoretic characterization of Aspergillus nidulans strains with chromosomal duplications

Cited by 6 publications

References 21 publications

Studies on the regeneration‐restore and karyotype of protoplast in Metarhizium anisopliae var. majus

Studies on the regeneration‐restore and karyotype of protoplast in Metarhizium anisopliae var. majus

Genetic variability and host specialization among brazilian populations of Ceratocystis fimbriata s.l.

Molecular Evolution of Aspergillus

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