Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH)

Wasko, Adriane Pinto; Galetti, Pedro Manoel

doi:10.1590/s1415-47572000000100025

Cited by 36 publications

(25 citation statements)

References 30 publications

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Section: Comp Cytogenet 2010 4(2)supporting

confidence: 83%

“…Similar results were found comparing the studies with silver nitrate staining (Margarido, Galetti, 1996) and FISH with an 18S rDNA probe (Wasko, Galetti, 2000), with the occurrence of two NORs (rDNA sites) in the end of the long arm of a unique large submetacentric pair in all seven species of Brycon analyzed. Furthermore, the FISH observations revealed very week additional signals in the interstitial position in two chromosome pairs, contrasting with the results described by Wasko, Galetti (2000) for other Brycon species. The FISH technique using 18S rDNA sequences has identifi ed a higher number of major ribosomal cistrons than the Ag-NOR technique in several species of fi sh: Salmo trutta Linnaeus, 1758 (Pendás et al, (Nirchio et al, 2003), Lebias fasciata Valenciennes, 1821 (Tigano et al, 2004) and Triportheus venezuelensis Spix, 1829 (Nirchio et al, 2007).…”

Section: Comp Cytogenet 2010 4(2)supporting

confidence: 83%

“…These differences may be important cytogenetic markers that could be used for differentiation of populations and/or species of Brycon. A remarkable stability of the Nucleolar Organizer Region (NOR) pattern, characterized by the presence of NORs in a single chromosome pair, has been recognized as a common characteristic of all Brycon species studied and proposed as a primitive feature in the subfamily Bryconinae (Wasko, Galetti, 2000). In B. amazonicus, metaphases exposed to silver impregnation also showed only one NOR-bearing chromosome pair, located in the terminal region of the long arms of the second pair of subtelocentric chromosomes, which correspond in number and locations to those found in other species of this genus analyzed to date (Table 1).…”

Section: Comp Cytogenet 2010 4(2)mentioning

confidence: 99%

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Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela

Mariguela¹,

Nirchio²,

Ron³

et al. 2010

CCG

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Abstract.A cytogenetic analysis by conventional Giemsa staining, silver staining, C-banding, and fl uorescence in situ hybridization (FISH) was carried out on Brycon amazonicus from Caicara del Orinoco, Venezuela. The karyotype of this species is characterized by the presence of 2n = 50 chromosomes, a karyotypic formula 22m+14sm+14st, and a fundamental number of 100 chromosomal arms. Nucleolar organizer regions (NORs) and 18S rDNA genes are located in the terminal regions of the long arms of the second pair of subtelocentric chromosomes, corresponding to pair 13. C-banding revealed heterochromatin distribution in only seven chromosome pairs. The largest metacentric pair (number 1) possesses a paracentromeric block equilocally distributed in both chromosome arms, whereas in pairs 12 to 17 positive C band blocks were located in the paracentromeric region of the long arm, close to the centromere. Analysis performed with 5S rDNA gene revealed a terminal site located on the short arm of one small submetacentric chromosome (pair 15) corroborating previous studies with this repetitive gene showing an apparent conservation of 5S rDNA in the genome of these fi sh species. The data obtained in this study reinforce the chromosomal conservativeness in the species of the genus Brycon, related not only to the macro-chromosomal structure (diploid number, karyotypic formula, and fundamental number), but also to the repeated DNAs, such as heterochromatic regions and ribosomal DNAs. These data will contribute to a better understanding of chromosomal evolution in both Brycon and Characidae fi shes.

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Section: Comp Cytogenet 2010 4(2)supporting

confidence: 83%

Section: Comp Cytogenet 2010 4(2)supporting

confidence: 83%

Section: Comp Cytogenet 2010 4(2)mentioning

confidence: 99%

See 1 more Smart Citation

Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela

Mariguela¹,

Nirchio²,

Ron³

et al. 2010

CCG

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“…In a number of fish species, NORs have been found to be associated with C-band regions (Pendas et al 1993;Martinez et al 1996;Wasko and Galetti 2000;Kushwaha et al 2011). The significance of association is not clear but it indicates that small portions of heterochromatin may be genetically active and important for gene expression (Rishi and Rishi 1992).…”

Section: Resultsmentioning

confidence: 99%

Karyological and molecular diversity in three freshwater species of the genusChanna(Teleostei, Perciformes) from India

et al. 2013

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Genetic variability was assessed among three species of genus Channa, namely C. gachua, C. punctata and C. striata, using karyological tools including karyomorphology, silver nitrate and chromomycin A 3 staining of nucleolar organizer regions (NORs) and C-banding. Additionally, sequence variation in partial 18S and divergent domain 1 of 28S ribosomal DNA was utilized for inter-species diversity assessment. Diploid chromosome numbers of 32, 40 and 52 in the three species were observed and the karyotype formula was derived as 18m + 12sm + 2st (fundamental arm number [FN] = 62); 6m + 2sm + 10st + 22t (FN = 48) and 12m + 10sm + 14st + 16t (FN = 86) in C. punctata, C. striata and C. gachua, respectively. Similarly, variations were also observed in number and location of silver and chromomycin A 3 stained NORs and C-banding patterns. DNA sequencing generated sizes for 18S of 243, 248, 245 bp and for 28S of 401, 392 and 393 bp in C. gachua, C. punctata and C. striata, respectively. The results indicated that the cytogenetic and molecular markers are useful for the assessment of genetic diversity among the fish species and could provide valuable information for germplasm evaluation and ex situ conservation.

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“…NORs polymorphic sizes located between homologous chromosomes could be explained by spontaneous events like as duplication, deletion and unequal crossing over (Gold et al 1990). Heteromorphism in size of NORs has been reported in neotropical fish frequently (Moreira-Filho et al 1984, Jesus and Moreira Filho 2000, Wasko and Galetti 2000, Hatanaka and Galetti 2004. Concluding, although Prochilodus lineatus was observed a conservational karyotypic in many different populations from Paraná River, the location and variability in size and number of NORs sites seems to be an unstable condition among differents populations.…”

Section: Resultsmentioning

confidence: 94%

Chromosomal Characterization of Prochilodus lineatus from Parana River, Corrientes, Argentina. I. B Chromosomes and NOR Banding

2011

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SummaryThe fish of the genus Prochilodus, commonly known as sábalo, are of great economic and ecological importance. Different species of this genus are widely distributed throughout all the basins of South America. Cytogenetic analysis performed on specimens of Prochilodus lineatus from Puerto Abras (Paraná River, Argentina), revealed the occurrence of 54 chromosomes of metasubmetacentric type, together with the presence of small supernumerary chromosomes with intra and interindividual variations. The use of the 18S rDNA probe confirmed the results obtained with AgNORs, identifying the presence of a metacentric chromosome pair with ribosomal cistrons in interstitial position in the long arm. Through both of these techniques are noted differences in the size of the nucleolar regions, this variation is probably related to the occurrence of events of duplication and/or deletion. Key words Fish, Prochilodus lineatus, B chromosomes, NOR, 18S rDNA.The genus Prochilodus is widely distributed in South America (Leccia 1972). Their economic value of inland fisheries in many South American countries and their anatomical peculiarities, point out interesting material for phylogenetic studies within Characiformes. Prochilodus lineatus known as sábalo or curimbatá represents in Argentina the most abundant fish resources from the Paraná River is an illiophagus fish, like other species of the family Prochilodontidae. From an economic point of view, easier cultivation in several systems and water qualities is, and the fish also has high protein meat and a balanced content of essential amino acids (Lessi 1968). Most of the appreciated reports which concern of P. lineatus karyotypes analyzed were specimens from Brazilian territory. Pauls andBertollo (1983, 1990) found in animals from different rivers of Paraná River basin diploid number of 54 chromosomes, fundamental number (NF) equal to 108 and a karyotype consisting of chromosomes of meta-submetacentric type. Other authors (Oliveira et al. 1997, Cavallaro et al. 2000 analyzed in this species the frequency and inheritance of chromosomes Bs. On the other hand, Jesus et al. (2003) achieved the molecular characterized 2 families of satellite DNA and its chromosomal location.Due to the limited information of the population of P. lineatus in Argentina, the objectives of this study are to obtain information about the karyotypic characteristics of the species and determine the chromosomal location of 18S ribosomal RNA genes as possible genetic marker for this purpose was used fluorescent in situ hybridization (FISH) and staining with silver nitrate.

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Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH)

Cited by 36 publications

References 30 publications

Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela

Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela

Karyological and molecular diversity in three freshwater species of the genusChanna(Teleostei, Perciformes) from India

Chromosomal Characterization of Prochilodus lineatus from Parana River, Corrientes, Argentina. I. B Chromosomes and NOR Banding

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