Hematoxylin: a simple, multiple-use dye for chromosome analysis

Guerra, Marcelo

doi:10.1590/s1415-47571999000100016

Cited by 19 publications

(13 citation statements)

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“…Somatic chromosomes were studied in root meristems pretreated in 0.05 % colchicine at room temperature for 3 h. These were then fixed in Carnoy solution (ethanol 96 %: glacial acetic acid; 3:1) for 24 h and stored in a freezer at -20°C (Sharma & Sharma 1972). Root tips were stained by hematoxylin-iron alum 4 % for 30 minutes and squashed with 45 % acetic acid (Guerra 1999). All slides were examined under a Nikon OPTOPHOT-2 and photographed by a Moticam 2300 digital camera.…”

Section: Methodsmentioning

confidence: 99%

Meiotic behaviour, karyotype analyses and pollen viability in species ofTamarix(Tamaricaceae)

Samadi¹,

Ghaffari

Akhani³

2013

Willdenowia

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Section: Methodsmentioning

confidence: 99%

Meiotic behaviour, karyotype analyses and pollen viability in species ofTamarix(Tamaricaceae)

Samadi¹,

Ghaffari

Akhani³

2013

Willdenowia

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“…For slide preparation, the material was hydrolyzed in 5 N HCl for 20-30 min at room temperature and stained with Giemsa 2% (Guerra, 1983) or hematoxylin at 1% (Guerra, 1999). Photomicrographs were taken with Kodak Imagelink or Agfa Copex Pan films, using a Leica DMRB photomicroscope adjusted to 25 ASA.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetics and cytotaxonomy of some Brazilian species of Cymbidioid orchids

Félix

Guerra

2000

Genet. Mol. Biol.

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The Cymbidioid phylad presents the widest chromosome number variation among orchids, with records varying from 2n = 10 in Psygmorchis pusilla to 2n = 168 in two species of Oncidium. In the present work, a total of 44 species were studied belonging to 20 Cymbidioid genera, as a contribution to clarifying the karyological evolution of the group. All the plants investigated were collected in Brazil, mainly in the northeast region. The chromosome variation found was similar to that previously registered in the literature. Chromosome numbers observed were: 2n = 54 (subtribe Eulophiinae), 2n = 44, 46, 92 (subtribe Cyrtopodiinae), 2n = 54, ca. 108 (subtribe Catasetinae), 2n = 52, ca. 96 (subtribe Zygopetalinae), 2n = 40, 80 (subtribe Lycastinae), 2n = 40, 42 (subtribe Maxillariinae), 2n = 40 (subtribe Stanhopeinae), 2n = 56 (subtribe Ornithocephalinae), and 2n = 12, 20, 30, 36, 42, 44, 56, 112, ca. 168 (subtribe Oncidiinae). Interphase nuclei varied widely from simple chromocenter to complex chromocenter types, with no apparent cytotaxonomic value. In the genera Catasetum and Oncidium, the terrestrial and lithophytic species presented higher ploidy levels than the epiphytic species, suggesting a higher adaptability of the polyploids to those habitats. The primary base number x = 7 seems to be associated to the haploid chromosome numbers of most Cymbidioid groups, although n = 7 was observed only in two extant genera of Oncidiinae. For each tribe, subtribe and genus the probable base numbers were discussed along with the possible relationships to the primary base number x 1 = 7 admitted for the whole phylad.

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“…Ross et al (1996) apud Fujji; Guerra (1998), usando uma solução de coloração semelhante, descobriram que reduzindo o tempo de hidrólise foi possível corar não só o nucléolo, mas também a heterocromatina pericentromérica de bivalentes de Arabidopsis. No entanto, colorações convencionais, por vezes, podem produzir falsos padrões de heterocromatina, sendo possível distinguir eucromatina condensada de heterocromatina, uma vez que a heterocromatina geralmente é melhor diferenciada por bandas C no procedimento com Giemsa do que por qualquer outro método (Guerra, 1988).…”

Section: Introductionunclassified

“…Contudo, a variação no método de Giemsa produz padrões de bandas que são o reverso das bandas G, isso é, as bandas G escuras são as bandas R claras e vice-versa (Brammer et al, 2007). Além disso, conforme Guerra (1988), é possível localizar exatamente a região do cromossomo diretamente afetada, o que seria impossível com a coloração convencional. Semelhante a outras espécies, essas técnicas têm possibilitado compreender melhor as alterações cromossômicas que se estabeleceram em cada cariótipo.…”

Section: Introductionunclassified

“…Essa foi umas das primeiras aplicações dos corantes fluorescentes e, desde então, tem se tornado rotina nos laboratórios de citogenética. Do mesmo modo, a utilização desses fluorocromos permite verificar a ocorrência de processos evolutivos complexos, tais como inversão pericên-trica e duplicação em tandem, em análises tanto em célu-las mitóticas como em células meióticas (Guerra, 1988;Feitoza, 2008).…”

Section: Introductionunclassified

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Corantes comumente empregados na citogenética vegetal

2015

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ABSTRACT:The use of dyes in plant cytogenetics goes back many years, as research in the fields of cytology and histology has been constantly developed since the first cellular studies in the 19 th century. Initially they were taken from plant or animal sources, and now they are produced synthetically on a commercial scale. These dyes are classified in fluorescent and non-fluorescent, according to their chemical properties and the choice of use is based on the type of cell structure or cell group to be analyzed. The diversity of types and chemical compounds available in different dyes provides their application in advanced and classical cytogenetics studies. A review of their chemical properties and use is presented for the non-fluorescent dyes orcein, hematoxylin, Giemsa, and carmine; and for the fluorescent dyes 4',6-diamidino-2-phenylindole (DAPI), chromomycin A (CMA), fluorescein, and rhodamine.KEYWORDS: cell biology; cytology; histology; chemical compounds. RESUMO:O emprego dos corantes, na citogenética vegetal, data de muitos anos, uma vez que as pesquisas nas áreas da citologia e histologia vêm sendo desenvolvidas constantemente desde os primeiros estudos celulares no século XIX. Inicialmente, eram extraídos de fontes vegetais ou animais, sendo atualmente produzidos sinteticamente em escala comercial. Os corantes são classificados em não fluorescentes e fluorescentes, conforme suas propriedades químicas e a escolha de uso é de acordo com o tipo de estrutura celular ou grupo celular a ser analisado. A diversidade de tipos e compostos químicos existentes nos diferentes corantes proporciona sua aplicação em estudos avançados na citogenética clássica e molecular. Uma revisão de suas propriedades químicas e emprego é apresentada para os corantes não fluorescentes orceína, hematoxilina, Giemsa, carmin; e para os fluorescentes 4',6-diamidino-2-fenilindol (DAPI), cromomicina A (CMA), fluoresceína e rodamina.

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Hematoxylin: a simple, multiple-use dye for chromosome analysis

Cited by 19 publications

References 10 publications

Meiotic behaviour, karyotype analyses and pollen viability in species ofTamarix(Tamaricaceae)

Meiotic behaviour, karyotype analyses and pollen viability in species ofTamarix(Tamaricaceae)

Cytogenetics and cytotaxonomy of some Brazilian species of Cymbidioid orchids

Corantes comumente empregados na citogenética vegetal

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