Comparing a single observation with a larger sample through Levene's test

Cerqueira, Rui

doi:10.1590/s1415-47571998000200012

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Pearson’s test was used for numerical correlation. To further investigate the effect of HLA-G alleles on HLA-G isoform mRNA expression, the comparison of a single observation to the mean of the sample has been used ( 37 ). In this particular case, data are represented as mean with SD.…”

Section: Methodsmentioning

confidence: 99%

Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression

et al. 2017

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Not all asthmatic patients adequately respond to current available treatments, such as inhaled corticosteroids or omalizumab®. New treatments will aim to target the bronchial epithelium–immune response interaction using different pathways. HLA-G is involved in immunomodulation and may promote epithelial cell differentiation and proliferation. HLA-G protein has several isoforms generated by alternative splicing that might have differential functionalities. HLA-G protein expression and genetic polymorphisms have been reported to be associated with asthma. Our hypothesis is that bronchial epithelium from asthmatic patients displays less functional HLA-G isoforms. HLA-G transcriptional isoforms were quantified by real-time PCR in human bronchial epithelium cells (HBEC) grown in air–liquid interface culture obtained from five healthy controls (HC), seven patients with mild asthma (MA), and seven patients with severe asthma (SA). They were re-differentiated, and IL-13 exposure was used as a proxy for a pro-inflammatory cytokine. HLA-G protein expression was assessed by western blot analysis. HLA-G allele was typed by direct sequencing. Our results showed that both MA and SA display less functional HLA-G isoforms than HC (p < 0.05); in vitro HBEC re-differentiation from SA displays a particular isoform expression profile compared to MA and HC (p = 0.03); HLA-G*01:06 frequency in MA and SA was significantly higher than in the healthy population (p = 0.03 and p < 0.001, respectively); and IL-13 exposure had no impact on HLA-G expression. Our results support that an impaired expression of HLA-G isoforms in asthmatic patients could contribute to the loss of inflammation control and epithelium structural remodeling. Therefore, HLA-G might be an interesting alternative target for asthmatic patients not adequately responding to current drugs.

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Section: Methodsmentioning

confidence: 99%

Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression

et al. 2017

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“…In addition, an u posteriori Tukey test was done as a multiple comparisons test. Samples of 0. oniscus from the Northern Atlantic Forest were analysed morphometrically only in respect to the new species, using a modified t-test (Cerqueira, 1998) as a result of the small sample size. Principal component analysis was performed using the covariance matrix of the natural logarithm of measurements.…”

Section: Methodsmentioning

confidence: 99%

A new species of Oryzomys (Rodentia, Sigmondontinae) from southeast Brazil, with comments on the classification of the O. capito species group

Weksler¹,

Geise

Cerqueira³

1999

Zoological Journal of the Linnean Society

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< O O l O g i C d j h m d gst heLinnean Society (l999), 12.5: 415-462. With 6 fiLgures Article ID: zjls, 1997.0165, available online at http://M.Mnv.idealibrary.rom on I Df bl @ a A new species of Oryzomys (Rodentia, Sigmondontinae) fiom southeast Brazil, with comments on the classification of the 0. cupito species group A new species of Olyzomys is described for the coastal Atlantic region of Brazil. Oryzomys seuanezi sp. nov. was collected in eight localities throughout the Atlantic Forest from the south of the State of Bahia to the State of the Rio de Janeiro, mostly in lowlands. Belonging to the 0. capito species group, 0. seuanezi differs from the other species by its karyotype equal 2n=48, FN=60. A morphometric analysis of populations of some species of the group indicates a split between the samples of the Atlantic Forest and those from 0. cupito from Cerrados and Amazon in relation to size, the former being larger. The population from Amazonia and from the Cerrados belongs to the same species, 0. goeldii being a synonym of 0. cupito. 0. seuanezi sp. nov. is the sister taxon of 0. onkcus, forming an Atlantic Forest clade. The close relationship of this group and some Peruvian populations of 0. cupito, probably a new species, is postulated. 0 1999 The Liiinean Socictv of London ADDITIONAL KEY WORDS:-karyosystematics ~ biogeography --morphometry ~ distributionphylogeny.

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Comparing a single observation with a larger sample through Levene's test

Cited by 2 publications

References 28 publications

Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression

Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression

A new species of Oryzomys (Rodentia, Sigmondontinae) from southeast Brazil, with comments on the classification of the O. capito species group

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