Evaluation testicular fine needle aspiration cytology and serum testosterone levels in dogs

Souza, Fabiana Ferreira de; Leme, Denise Pereira; Uechi, Edilson; Trinca, Luzia A.; Lopes, Maria Denise

doi:10.1590/s1413-95962004000200004

Cited by 8 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors pointed out that cytological examination must be performed after proper anamnesis, testicular palpation and semen analysis; in addition, they confirmed that testicular FNA results were well correlated with histological findings. In other study [8], in addition to clinical exam and semen analysis, testicular volume carried out before testicular FNA provided valuable information about the spermatogenesis of four male dogs.…”

Section: Introductionmentioning

confidence: 99%

Testicular cytology by fine needle aspiration in domestic cats

Leme

Visacre²,

Castro

et al. 2018

Theriogenology

View full text Add to dashboard Cite

In cases where semen collection in tom-cats is not possible, FNA of testes is the alternative to evaluate sperm production. Although this technique for the diagnosis of fertility problems has been well discussed in other mammals (men, dogs, stallions), data for domestic cats are limited. Therefore, the aim of this study was to verify the reliability of FNA using needles of small diameters (22G and 29G) in testes of domestic cats of different ages to assess the spermatogenesis status and to present description of germ cells and Sertoli cells for cytological examinations. Thirty-four mixed breed cats aged between four months and two years presented for neutering to a Veterinary Hospital were used in this study. Under general anesthesia, testicular measures and FNA were followed by orchiectomy and imprints of the parenchyma of testes and epididymides. Cats were assigned into 3 groups: (G1) 10 cats aged less than 6 months, (G2) 14 cats aged between 6 months and one year and (G3) 10 cats aged more than one year. Cats weighted between 1.5 and 6.0 kg. The mean testicular volume (TV cm) was 0.55 (G1), 1.18 (G2) and 2.66 (G3). Hemorrhages in the needle path were observed in more than 70% of testes. Few samples (4/68) were excluded due to blood contamination. All germ cells and Sertoli cells were identified and quantified in imprint and FNA smears. Incomplete spermatogenesis was observed in cats aged less than 6 months using both techniques (FNA and imprint); therefore, testicular FNA should not be recommended for cats at this age. Complete spermatogenesis was found in 64% of cats aged from 6 months up to one year and in all cats aged more than one year. There were no differences of Sertoli cell Index (SEI) and Spermatic Index (SI) between FNA and imprints of cats older than 6 months. In conclusion, FNA using needles of small diameter in the testes of domestic cats is viable, reliable and can be used as a tool for the analysis of the spermatogenesis status of cats older than 6 months, mainly in cases in which semen collection is not possible.

show abstract

Section: Introductionmentioning

confidence: 99%

Testicular cytology by fine needle aspiration in domestic cats

Leme

Visacre²,

Castro

et al. 2018

Theriogenology

View full text Add to dashboard Cite

show abstract

“…2005) which has been proposed as a diagnostic tool in infertile male dogs (Dhalbom et al. 1997; De Souza et al. 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Testicular fine needle aspiration (TFNA) is a less invasive technique widely performed in human andrology to evaluate spermatogenesis. This is a quick, simple, economic and useful analysis for immediate evaluation of spermato-genesis in human infertility (Mahajan et al 1999;Bettella et al 2005) which has been proposed as a diagnostic tool in infertile male dogs (Dhalbom et al 1997;De Souza et al 2004). Although remarkably less invasive than open biopsy (Dhalbom et al 1997), its diagnostic value is almost as high as that of testicular biopsy (Qublan et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Clinical Use of Testicular Fine Needle Aspiration Cytology in Oligozoospermic and Azoospermic Dogs

Romagnoli

Bonaccini

Stelletta

et al. 2009

Reprod Domestic Animals

View full text Add to dashboard Cite

Clinical investigation of canine testicular function is complicated by the difficulty in the evaluation of seminiferous tubules. Until recently, testicular biopsy was the only diagnostic option for dogs with persistent oligo/azoospermia. In human andrology, testicular fine needle aspiration (TFNA) is currently considered a useful method in the evaluation of azoospermia and severe oligozoospermia, and has long replaced classical biopsy to evaluate spermatogenesis. In order to verify its diagnostic efficacy for the clinical approach to canine oligo- or azoospermia, TFNA was performed in seven adult (two oligozoospermic and five azoospermic) dogs. After sedation, a fine (21-23 gauge) butterfly needle connected to a 50-ml syringe was inserted into each testicle; strong suction was applied and the aspirated fluid squirted on a glass slide, smeared out, air-dried and stained with a modified May-Grunwald-Giemsa. Under light microscopy, Sertoli cells (all those found in each investigated field) and spermatogenic cells (n = 100) were counted on each smear in order to differentiate spermatogonia, primary spermatocytes, secondary spermatocytes, early spermatids, late spermatids and spermatozoa, and calculate their relative percentages. Cytological analysis showed the following testicular pictures: normal spermatogenesis (compatible with obstruction of the seminal ducts), hypospermatogenesis, maturative disturbances and Sertoli cell-only syndrome. Two dogs with an obstructive lesion were treated with corticosteroids; one of them recovered and sired two litters of puppies.

show abstract

“…Some authors have reported that these are useful predictors for daily spermatozoa production and, consequently, for fertility in bulls, rams, dogs, and other species including humans. [35][36][37] In this study, it was shown that the sperm counts of healthy animals but not of chronic chagasic dogs are dependent on the scrotal perimeter. This finding suggests that the scrotal circumference could be useful for predicting the production and ejaculation of sperm only in healthy males.…”

Section: Discussionmentioning

confidence: 99%

Sperm Morphological Features Associated with Chronic Chagas Disease in the Semen of Experimentally Infected Dogs

Rodríguez-Morales

Pedro-Martínez

Hernández-Pichardo

et al. 2014

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The presence of trypanosomatids in the reproductive systems of different mammals (causing genital lesions in the acute stage of the disease) may predispose the animals to low semen quality. However, there are no studies examining the alterations in the sperm morphological features in the chronic stage of Trypanosoma cruzi infection. Knowledge of these aspects is important to understand the other ways of transmission of the Chagas disease. Progressive motility, mass motility, concentration, and sperm morphology of 84 ejaculates of dogs that were chronically infected with T. cruzi were evaluated. Most of the findings were consistent with the reference values and with those obtained from healthy control dogs. The scrotal circumference was not correlated with spermatozoa concentration in the infected animals. In conclusion, the T. cruzi Ninoa (MHOM/MX/1994/Ninoa) strain does not cause significant alterations in the semen quality of dogs experiencing chronic Chagas disease (at concentrations of 5

show abstract

Evaluation testicular fine needle aspiration cytology and serum testosterone levels in dogs

Cited by 8 publications

References 0 publications

Testicular cytology by fine needle aspiration in domestic cats

Testicular cytology by fine needle aspiration in domestic cats

Clinical Use of Testicular Fine Needle Aspiration Cytology in Oligozoospermic and Azoospermic Dogs

Sperm Morphological Features Associated with Chronic Chagas Disease in the Semen of Experimentally Infected Dogs

Contact Info

Product

Resources

About