Análise cromossômica e molecular do javali europeu Sus scrofa scrofa e do suíno doméstico Sus scrofa domesticus

Gimenez, Danilo Lucano; Mota, Lígia Souza Lima Silveira da; Curi, Rogério Abdallah; Rosa, Guilherme Jordão de Magalhães; Gimenes, Marcos Aparecido; Lopes, Catalina Romero; Lucca, Edmundo José de

doi:10.1590/s1413-95962003000200009

Cited by 12 publications

(11 citation statements)

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“…The average of 6.0 polymorphic bands for any amplifying primer is higher than the 3.0 obtained by Bouzat (2001) in great rhea; the 4.6 obtained by Albuquerque et al (2006) in water buffaloes. It was similar to the 5.5 bands obtained by Serrano et al (2004) in cattle and to the 5.8 obtained by Prim et al (2003) in bullfrogs and was equal to that found by Godoy et al (2005) in ostriches. The number of bands/primer was different from those found by Spritze et al (2003) in Crioulo cattle; from Paiva et al (2005), who found 2.8 bands/primer in Brazilian sheep and Gimenez et al (2003), who find the highest number of bands/primer: 10.7 in wild boars, pigs and their hybrids and from Leipoldt et al (1996), who found 22.5 bands/primer in lemurs.…”

Section: Resultssupporting

confidence: 91%

“…This percentage was similar to that reported by Godoy et al (2005) in ostriches, where they could select 38.9% of the primers according to the quality of the bands they produced. This result was lower than that obtained with water buffaloes (Albuquerque et al, 2006), wild boars and pigs (Gimenez et al, 2003), where 52.4 and 70.1% of the primers resulted in polymorphic fragments. This percentage was higher than that obtained in sheep, where 13.6% of the primers were functional (Paiva et al, 2005).…”

Section: Resultssupporting

confidence: 88%

“…RAPD-PCR (Random Amplified Polymorphic DNA PCR) can help to know the diversity and the genetic correlations; previous knowledge of the genetic sequence of the studied species is not needed, only a small amount of DNA is required for analysis and it is efficient and cheap (Welsh & Mc Clelland, 1990;Williams et al, 1990;Hadrys et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique

Antunes¹,

Machado²,

Serão³

et al. 2010

R. Bras. Zootec.

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-The genetic diversity was analyzed among spotted paca (Agouti paca) from three commercial flocks located in Brazil. As the genome of this species is unknown, the RAPD-PCR technique was used. Ten primers generated sixty polymorphic bands. The among and within population genetic variability estimated by analysis of molecular variance (AMOVA) was 12.55 and 87.45%, respectively. The shortest Nei distance value was 11.76% among the Carangola (CG) and São Francisco do Glória (SF) populations. This value can be explained by the exchange of reproduction males and females between the two geographically close breeding sites. The analysis of principal components showed well defined and structured groups aggregating animals according their population of origin, with some exceptions. Lower diversity was found in the São Francisco population than in the Carangola and Castelo (CS) populations. This result suggested the variability is better conserved in breeding farms with fifty or sixty animals (CG and CS) than in the breeding farm with a dozen animals (SF). The RADP-PCR technique proved to be informative for the quantification of among and within population genetic variability of the spotted paca. The phenogram generated by UPGMA using the NTSYS-PC software from the Nei Distance, grouped CG and SF on a single branch connected to the CS, with 76 and 100% accuracy, respectively, to the bootstrap. This result was not only consistent with the historical and geographical information on flocks, but also shows the need for periodic reproductive male replacement. Future studies should be developed with co-dominant markers and include spotted paca from more distant places.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 88%

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Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique

Antunes¹,

Machado²,

Serão³

et al. 2010

R. Bras. Zootec.

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“…The meat was well accepted by consumers and the market expanded with the introduction of purebreds from France. Commercial farms currently exist in several Brazilian states (GIMENEZ et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Parasitic helminths of the digestive system of wild boars bred in captivity

Silva

Müller

2013

Rev. Bras. Parasitol. Vet.

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This study aimed to identify the parasites that inhabit the digestive system of Sus scrofa scrofa from a commercial breeding facility in southern Brazil, and reports the first occurrence of Trichostrongylus colubriformis in wild boars. The gastrointestinal tracts of 40 wild boars from a commercial breeding facility were collected and individualized during slaughter in a cold-storage slaughterhouse. Out of this total, 87.5% were parasitized by the helminths Ascaris suum, Trichostrongylus colubriformis, Oesophagostomum dentatum and Trichuris suis. T. colubriformis presented a prevalence of 45%, mean intensity of 28.4 and mean abundance of 12.8. The data from this study showed that T. colubriformis not only has a capacity to develop in the small intestines of wild boars, but also adapts well to animals raised in captivity, thus representing a possible cause of economic loss in commercial wild boar farming.Keywords: Wild boar, Sus scrofa scrofa, helminths, Trichostrongylus colubriformis. gastrointestinal tract. ResumoO estudo teve por objetivo identificar os parasitos que habitam o sistema digestório de Sus scrofa scrofa provenientes de criatório comercial do sul do Brasil, reportando a primeira ocorrência de Trichostrongylus colubriformis em javalis. Foram coletados e individualizados os tratos gastrintestinais de 40 javalis oriundos de criatório comercial durante abate em frigorífico. Destes, 87,5% estavam parasitados por helmintos, sendo eles, Ascaris suum, Trichostrongylus colubriformis, Oesophagostomum dentatum e Trichuris suis. T. colubriformis apresentou prevalência de 45%, intensidade média de 28,4 e abundância média de 12,8. Os dados deste trabalho demonstram que T. colubriformis além da capacidade de desenvolvimento no intestino delgado de javalis, adapta-se bem às criações, representando uma possível causa de perdas econômicas nas criações de javalis.

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“…Currently, distinction between the pure and hybrid animals is made not only by phenotype observation, but also by the means of number of chromosomes analysis in diploid cells. In some cases, these methods are insufficient for safe determination of animal origin, since the hybrid phenotypes could be close to the pure animals and the chromosomal analysis does not determine the individual pureness but population pureness (Gimenez et al, 2003). Other molecular methods, as molecular markers, can collaborate with this distinction.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)

Silva

Lui

Band

et al. 2011

Braz. arch. biol. technol.

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Análise cromossômica e molecular do javali europeu Sus scrofa scrofa e do suíno doméstico Sus scrofa domesticus

Cited by 12 publications

References 0 publications

Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique

Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique

Parasitic helminths of the digestive system of wild boars bred in captivity

Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)

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