Preservation of goat preantral follicles in saline or coconut water solution

Costa, Sónia; Santos, Regiane R.; Ferreira, Marcos; Machado, Vanessa Porto; Rodrigues, Ana Paula Ribeiro; Ohashi, Otávio Mitio; Figueiredo, José Ricardo de

doi:10.1590/s1413-95962002000600009

Cited by 11 publications

(8 citation statements)

References 15 publications

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“…Other experiments revealed that SS did not differ from TCM‐199 or coconut water (Costa et al. ) in its effect on PAF isolation. Both experiments mentioned are consistent with the results obtained in the present work, where no differences were observed between the solutions used.…”

Section: Discussionmentioning

confidence: 80%

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Leonel

Bento-Silva

Ambrozio

et al. 2013

Reprod Domestic Animals

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The aim of this study was to test the use of mechanical and mechanical-enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase). Incubation times of 10 and 20 min were employed. In the first group, 4.1 ± 4.9 PAFs were harvested with the mechanical-enzymatic method vs 71.1 ± 19.2 with the mechanical procedure, showing a significant difference between methods; using SS and PBS, these numbers were 35.7 ± 34.3 and 39.6 ± 39.6, respectively, with no significant difference between solutions. In the second group, there was significant difference between methods, with 7.1 ± 10.6 follicles harvested with the mechanical-enzymatic method vs 63.2 ± 22.9 with the mechanical procedure; using SS and PBS, means were 35.5 ± 36.4 and 34.9 ± 31.1, respectively. The mechanical method proved more effective than the mechanical-enzymatic approach. Both SS and PBS can be used as a media for equine PAFs preparation.

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Section: Discussionmentioning

confidence: 80%

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Leonel

Bento-Silva

Ambrozio

et al. 2013

Reprod Domestic Animals

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“…Other authors have reported that the coconut water can be as effective as saline for ovarian follicle preservation under certain conditions 16 . This information would justify the difficulty to highlight differences between the preservation solutions such as Ringer lactate and Belzer.…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 98%

Preliminary study of coconut water for graft tissues preservation in transplantation

César

Petroianu

Vasconcelos

et al. 2015

Rev. Col. Bras. Cir.

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OBJECTIVE: to verify the effectiveness of coconut water in preserving tissues for transplant. METHODS: Fifty male Wistar rats were randomly distributed in five groups, according to the following preservation solutions for tissue grafts: Group 1: Lactated Ringer; Group 2: Belzer solution; Group 3: mature coconut water; Group 4: green coconut water; Group 5: modified coconut water. In Group 5, the green coconut water has been modified like the Belzer solution. From each animal we harvasted the spleen, ovaries and skin of the back segment. These tissues were preserved for six hours in one of the solutions. Then, the grafts were reimplanted. The recovery of the function of the implanted tissues was assessed 90 days after surgery, by splenic scintigraphy and blood exame. The implanted tissues were collected for histopathological examination. RESULTS: The serum levels did not differ among groups, except for the animals in Group 5, which showed higher levels of IgG than Group 1, and differences in relation to FSH between groups 1 and 2 (p <0.001), 4 and 2 (p = 0.03) and 5 and 2 (p = 0.01). The splenic scintigraphy was not different between groups. The ovarian tissue was better preserved in mature coconut water (p <0.007). CONCLUSION: the coconut water-based solutions preserves spleen, ovary, and rat skin for six hours, maintaining their normal function.

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“…Regarding the ovine species, the best results were obtained when the medium was replaced every 6 days. Studies with chilled (Costa et al. 2002; Matos et al.…”

Section: Discussionmentioning

confidence: 99%

“…2002). Furthermore, studies performed in our laboratory with chilled (Costa et al. 2002; Matos et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Medium Replacement Interval on the Viability, Growth and In Vitro Maturation of Isolated Caprine and Ovine Pre‐Antral Follicles

Magalhães

Fernandes

Mororó

et al. 2011

Reprod Domestic Animals

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The aim of the present study was to evaluate the effects of different medium replacement intervals on the viability, antral cavity formation, growth and in vitro maturation (IVM) of oocytes from caprine and ovine pre-antral follicles. Pre-antral ovarian follicles (≥ 150 μm) were isolated from the ovarian cortex of goats and sheep and were individually cultured for 24 days using two different medium replacement intervals [2 days (T(1) ) or 6 days (T(2) )]. Follicle development was evaluated on the basis of antral cavity formation, increases in follicular diameter and the presence of healthy cumulus oocyte complexes and fully grown oocytes. For caprine species, results showed a higher percentage (p<0.05) of viable follicles in T(1) than T(2) from day 6 until the end of the culture. In addition, when comparing both treatments after the same culture duration, the rate of antrum formation was significantly higher in T(1) than in T(2) from day 12 onwards. Yet, in ovines, when both treatments were compared on day 24 of the culture, there were more viable follicles in T(2) than in T(1) (p<0.05). In the caprine species, percentages of fully grown oocytes (≥ 110 μm) acceptable for IVM after 24 days of culture were significantly higher in normal follicles cultured in T(1) (30.0%) than in T(2) (6.7%; p<0.05). On the other hand, in ovines, at the end of the culture, the percentage of oocytes destined for IVM was higher in T(2) than in T(1) (23.5% vs 2.9%; p<0.05). In conclusion, under the same conditions, the frequency of medium replacement significantly affected the in vitro development of caprine and ovine pre-antral follicles. To improve the efficiency of the culture system, the medium must be replaced every 2 and 6 days for goat and sheep pre-antral follicles, respectively.

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Preservation of goat preantral follicles in saline or coconut water solution

Cited by 11 publications

References 15 publications

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Methods for Equine Preantral Follicles Isolation: Quantitative Aspects

Preliminary study of coconut water for graft tissues preservation in transplantation

Effect of the Medium Replacement Interval on the Viability, Growth and In Vitro Maturation of Isolated Caprine and Ovine Pre‐Antral Follicles

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