Cytochemical aspects of the peripheral blood cells of Oreochromis (Tilapia) niloticus. (Linnaeus, 1758) (Cichlidae, Teleostei): part II

Ueda, Ivete Kotomi; Egami, Mizue Imoto; Sasso, Wilson da Silva; Matushima, Eliana Reiko

doi:10.1590/s1413-95962001000600005

Cited by 31 publications

(59 citation statements)

References 26 publications

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“…The intense staining for myeloperoxidase, acid phosphatase and esterase observed in neutrophil has also been reported in such a granulocyte in other fish species [9,35,38]. The present study found that the staining for ACP is intense in young neutrophils whereas it is only slight or even negative in mature neutrophils.…”

Section: Cytochemistrysupporting

confidence: 86%

See 1 more Smart Citation

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Silva

Egami

Santos

et al. 2011

Fish & Shellfish Immunology

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Section: Cytochemistrysupporting

confidence: 86%

“…This inclusion has been referred to as fundamental to the mechanism of phagocytosis [5,11,35]. Our findings show the presence of glycogen neutrophils.…”

Section: Cytochemistrysupporting

confidence: 60%

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Silva

Egami

Santos

et al. 2011

Fish & Shellfish Immunology

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“…Thrombocytes have been described as the second most abundant blood cells in fish, after erythrocytes (UEDA et al, 2001). However, in all groups of this study, the number of thrombocytes was lower than the absolute number of leukocytes.…”

Section: Discussioncontrasting

confidence: 55%

Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols

Weinert

Volpato

Costa

et al. 2015

SCA

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Clinical hematology facilitates the diagnosis of disease and can act as a prognostic indicator of pathological conditions in fish. The aim of the present study was to evaluate hematological parameters of Nile tilapia (Oreochromis niloticus) subjected to different anesthetics and anticoagulants. Thirty apparently healthy fishes (average weight of 473 ± 35. 50 g and mean total length of 29. 33 ± 0. 37 cm), were selected from the local commercial fish farm in the Lages municipality (Santa Catarina, Brazil). The animals were randomly divided into three groups of 10. In two groups, anesthesia was induced with eugenol (70 mg·L -1 ) (EG) and Benzocaine hydrochloride (100 mg·L -1 ) (BG), respectively. Anesthesia was not administered to fish of the third group (CG/control group). Blood samples were obtained by venipuncture of the caudal vessels and placed into microtubes containing sodium heparin or Na 2 EDTA for further analysis. The results were analyzed by Sigma Stat for Windows, the paired t-test for significant differences between anticoagulants of the same group, and analysis of variance followed by the Tukey test for comparison of means between groups (p ≤ 0. 05). Most of the observed changes in the erythrogram were significantly higher for the anticoagulant heparin and benzocaine group in comparison to the control group. However, the values obtained for the leukogram were significantly higher for all groups subjected to the Na 2 EDTA anticoagulant, suggesting that heparin may cause cell clumping. The results suggest that the anesthetics under investigation effectively minimizes the effects of stress caused by handling and invasive procedures, and that the anticoagulant heparin causes less hemolysis in comparison to Na 2 EDTA for Nile tilapia. Thus, the hematological variations attributed to different anesthetic protocols and/or different anticoagulants should be considered for the species Oreochromis niloticus. Key words: Anesthetics, anticoagulants, hematology, Oreochromis niloticus, osmotic fragility ResumoA hematologia clínica é uma ferramenta que auxilia na realização do diagnóstico de doenças e pode atuar como um indicador prognóstico das condições patológicas de peixes. O objetivo deste estudo foi avaliar os valores hematológicos de tilápias do Nilo (Oreochromis niloticus) submetidas a diferentes anestésicos e amostras obtidas com diferentes anticoagulantes. Foram utilizados trinta peixes, com peso médio de 473±35,50 g e comprimento total médio de 29,33±0,37 cm, adquiridos de piscicultura comercial localizada no município de Lages (SC). Os animais foram divididos aleatoriamente em três grupos (n=10) conforme a forma de indução: grupo eugenol (GE) na concentração de 70 mg L -1 , grupo cloridrato de benzocaína (GB) na concentração de 100 mg L -1 e grupo controle (GC) sem o uso de anestésicos. As amostras de sangue foram obtidas por venopunção dos vasos caudais e acondicionadas em microtubos contendo heparina sódica ou Na 2 EDTA e posteriormente processadas. Os resultados foram avaliados pelo Sigma Sta...

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“…Peroxidases and the halogen ions that they produce within the phagocytic vacuoles of leucocytes form an effective anti-bacterial system in higher vertebrates. Therefore, the presence of peroxidase activity is a strong indication of anti-bacterial and phagocytic functions [8,11,12], with peroxidases being characteristic of mammalian neutrophils as well as the piscine equivalent, the heterophil [13][14][15][16]. Acid and alkaline phosphatases are lysosomal enzymes also involved in the phagocytic process, being discharged into the phagocytic vacuoles of leucocytes that have ingested bacteria and other particles [17].…”

Section: Discussionmentioning

confidence: 99%

“…Periodic acid Schiff's stains glycogen and glycoproteins, with the staining intensity increasing during cell maturation due to the accumulation of glycogen [20]. It has been suggested that the presence of glycogen is related to the phagocytic functions of certain cell types that require both endogenous and exogenous sources of energy [11].…”

Section: Discussionmentioning

confidence: 99%

Cytochemical characterisation of the leucocytes and thrombocytes from Murray cod (Maccullochella peelii peelii, Mitchell)

Shigdar

Harford²,

Ward

2009

Fish & Shellfish Immunology

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Cytochemical aspects of the peripheral blood cells of Oreochromis (Tilapia) niloticus. (Linnaeus, 1758) (Cichlidae, Teleostei): part II

Cited by 31 publications

References 26 publications

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

Hematology of Nile tilapia (Oreochromis niloticus) subjected to anesthesia and anticoagulation protocols

Cytochemical characterisation of the leucocytes and thrombocytes from Murray cod (Maccullochella peelii peelii, Mitchell)

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