In vitro viability of canine spermatozoa frozen in tris-fructose-citric acid extender with ethylene glycol

Vannucchi, Camila Infantosi; Santos, Silvia Edelweiss Crusco dos; Visintin, José Antônio

doi:10.1590/s1413-95961999000400007

Cited by 2 publications

(2 citation statements)

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“…These data showed that T2 preserved better the structural integrity of the plasma membrane than the two other treatments. The association of lactose-egg yolk extender with a cryoprotectant of low toxicity as EG, which has high permeability due to its low molecular weight (Vannucchi, 1999) is likely to better stabilize the structure of the plasma membranes. Based on the results of the present study and preliminary data from two above mentioned studies, a larger trial using EG as the cryoprotectant should be conduct.…”

Section: Discussionmentioning

confidence: 99%

“…However, the toxicity of glycerol (Fary, 1986;England, 1993) is leading to the need to elect other alternatives. Penetrating cryprotectants such as dimethyl sulphoxido (DMSO) (Olar, 1989) and ethylene glycol (Vannucchi et al, 1999) have been used for canine sperm preservation. Recent studies used some forms of amides, including methyl and dimethyl formamide (DMF) and acetamide as cryoagent for equine spermatozoa preservation (Hanada and Nagase, 1980;Snoek, 2003;Squires et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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In vitro evaluation of canine spermatozoa cryopreserved in different extenders

Oliveira¹,

Juliani²,

Marques³

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of postthawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling test. Lactose-egg yolk based extenders showed better cryoprotectant capability and dimethyl formamide was an alternative cryoprotectant agent for dog sperm cells.Keywords: dog, semen, cryopreservation, ethylene glycol, dimethyl formamide RESUMO Avaliou-se a eficácia de três diluidores, tris-gema com 5% de etileno glycol (T1), lactose-gema com 5% de etileno glicol (T2) e lactose-gema com 5% de dimetil-formamida (T3) na

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Section: Discussionmentioning

confidence: 99%