Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Ferreira, Carla Elizabeth de Oliveira; Raboni, Sônia Mara; Pereira, Luciane Aparecida; Nogueira, Meri Bordignon; Vidal, Luíne Rosele Renaud; Almeida, Sérgio Monteiro de

doi:10.1590/s1413-86702012000300009

Cited by 27 publications

(21 citation statements)

References 40 publications

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“…The results obtained from this study were consistent with those of previous studies, which have reported worldwide occurrence rates of viral co-infections ranging from 1.6 % to 19 % [3,12,18,[48][49][50][51]. As in previous studies [49,[51][52][53], RV ?…”

Section: Discussionsupporting

confidence: 93%

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

Jia

Zhong

et al. 2015

Arch Virol

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Rotavirus (RV), norovirus (NoV), sapovirus (SaV), human astrovirus (HAstV) and human adenovirus (HAdV) are significant because they are the most common pathogens that cause diarrhea in young children. The aim of this study was to investigate the genetic characteristics and compare the roles of these five viruses in outpatient children with diarrhea in Shanghai. A total of 436 fecal samples were collected from pediatric patients with acute gastroenteritis from January 2010 to December 2011. The selected samples were subjected to reverse transcription PCR (RT-PCR) or PCR to detect and genotype RV, NoV, SaV, HAstV and HAdV. RV (43.3 %, 189/436) was the most prevalent virus, followed by NoV (28.9 %, 126/436), HAdV (7.1 %, 31/436). HAstV (1.8 %, 8/436) and SaV (0.5 %, 2/436). The percentage of multiple infection cases was 14.9 % (65/436), and RV + NoV was the predominant mixed infection. The RV genotype combinations of P[8]G3 (52/189, 27.5 %), P[8]G1 (51/189, 26.9 %) and P[8]G9 (48/189, 25.4 %) occurred most frequently. The predominant NoV genotype was GII.4 (73.0 %, 92/126), and the majority of GII.4 clustered as GII.4-2006b (65.2 %, 60/92). Two of the SaV cases were identified as GI.2 and GII.1. All HAstV-positive samples belonged to HAstV-1. The predominant HAdV type was HAdV-41 (45.2 %, 14/31). This study clearly shows the diversity of the viral causative agents of acute gastroenteritis in outpatient children in Shanghai, which will provide baseline information for future vaccination strategies and development in this area.

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Section: Discussionsupporting

confidence: 93%

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

Jia

Zhong

et al. 2015

Arch Virol

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“…These viruses commonly infect young children, often without signs of clinical disease (67)(68)(69)(70)(71). Coinfections involving RV and other enteric viral or bacterial pathogens have been frequently reported, and there have been a few studies suggesting that coinfections with certain pathogens can alter GI disease severity (63,(72)(73)(74)(75)(76). However, little information is available concerning whether coinfection of young children, and particularly neonates, with RV and Aichi virus, astrovirus, or salivirus/klassevirus has an impact on disease symptoms.…”

Section: Discussionmentioning

confidence: 99%

Absence of Genetic Differences among G10P[11] Rotaviruses Associated with Asymptomatic and Symptomatic Neonatal Infections in Vellore, India

Libonati

Dennis

Ramani

et al. 2014

J Virol

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Rotaviruses (RVs) are leading causes of severe diarrhea and vomiting in infants and young children. RVs with G10P[11] genotype specificity have been associated with symptomatic and asymptomatic neonatal infections in Vellore, India. To identify possible viral genetic determinants responsible for differences in symptomology, the genome sequences of G10P[11] RVs in stool samples of 19 neonates with symptomatic infections and 20 neonates with asymptomatic infections were determined by Sanger and next-generation sequencing. The data showed that all 39 viruses had identical genotype constellations (G10-P[11]-I2-R2-C2-M2-A1-N1-T1-E2-H3), the same as those of the previously characterized symptomatic N155 Vellore isolate. The data also showed that the RNA and deduced protein sequences of all the Vellore G10P[11] viruses were nearly identical; no nucleotide or amino acid differences were found that correlated with symptomatic versus asymptomatic infection. Next-generation sequencing data revealed that some stool samples, both from neonates with symptomatic infections and from neonates with asymptomatic infections, also contained one or more positive-strand RNA viruses (Aichi virus, astrovirus, or salivirus/klassevirus) suspected of being potential causes of pediatric gastroenteritis. However, none of the positive-strand RNA viruses could be causally associated with the development of symptoms. These results indicate that the diversity of clinical symptoms in Vellore neonates does not result from genetic differences among G10P[11] RVs; instead, other undefined factors appear to influence whether neonates develop gastrointestinal disease symptoms. IMPORTANCERotavirus (RV) strains have been identified that preferentially replicate in neonates, in some cases, without causing gastrointestinal disease. Surveillance studies have established that G10P[11] RVs are a major cause of neonatal infection in Vellore, India, with half of infected neonates exhibiting symptoms. We used Sanger and next-generation sequencing technologies to contrast G10P[11] RVs recovered from symptomatic and asymptomatic neonates. Remarkably, the data showed that the RNA genomes of the viruses were virtually indistinguishable and lacked any differences that could explain the diversity of clinical outcomes among infected Vellore neonates. The sequencing results also indicated that some symptomatic and some asymptomatic Vellore neonates were infected with other enteric viruses (Aichi virus, astrovirus, salvirus/klassevirus); however, none could be correlated with the presence of symptoms in neonates. Together, our findings suggest that other poorly defined factors, not connected to the genetic makeup of the Vellore G10P[11] viruses, influence whether neonates develop gastrointestinal disease symptoms.

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“…İnfeksiyöz ishaller arasinda viral gastroenteritler önemli bir yer tutmaktadir. Bu gastroenteritlerde rastlanan patojenler siklikla rotavirüs ve adenovirüslerdir [2][3][4].…”

Section: Akut Gastroenterit Vakalarında Rotavirüs Ve Adenovirüs Sıklıunclassified

Investigation of the frequency of rotavirus and adenovirus in acute gastroenteritis cases

Çaycı¹,

Yılmaz²,

Bırınci³

2017

Pau Med J

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ÖzetAmaç: Çalişmamizda hastanemize başvuran akut gastroenteritli hastalarda rotavirüs ve adenovirüs sikliğini, cinsiyet, yaş ve mevsimlere göre dağilimlarini retrospektif olarak belirlemeyi amaçladik. Gereç ve yöntem: Mikrobiyoloji Laboratuvarina Ocak 2014-Haziran 2015 tarihleri arasinda çeşitli poliklinik ve servislerden gönderilen, akut gastroenterit ön tanili 2355 hastanin dişki örneğinde rotavirüs ve adenovirüs antijenleri, renkli kromatografik yöntemle çalişan Rota-Adeno Card Test kiti kullanilarak araştirildi. Bulgular: Rotavirüs araştirilan 1101 örneğin, 129'unda (%11,7) pozitiflik saptanmiştir. Adenovirüs ise 1254 örneğin 42'sinde (%3,3) pozitif saptanmiştir. İki örnekte, her iki virüs de saptanmiştir. Rotavirus ve adenovirus pozitiflik orani en sik 13-24 ay grubunda saptanirken, rotavirüs ve adenovirüse bağli enfeksiyonlarin kiş ve ilkbahar aylarinda daha sik görüldüğü saptanmiştir. Yaşa ve mevsimlere göre rotavirüs ve adeno virüs dağilimi incelendiğinde istatiksel olarak anlamli bir ilişki saptanmamiştir (p>0,05). Sonuç: Dişki örneklerinde rotavirüs ve adenovirüs antijenlerinin araştirilmasi epidemiyolojik amaçlar ve gereksiz antibiyotik kullanimini önleme açisindan önemlidir. Pam Tıp Derg 2017;(1):61-65Anahtar sözcükler: Rotavirüs, adenovirus, gastroenterit. AbstractPurpose: We aimed to evaluate the prevalance of rotavirus and adenovirus, and their distribution according to gender, age and season, retrospectively in patients with acute gastroenteritis. Materials and methods: Samples from 2355 patients with suspected acute gastroenteritis, sent from different clinics and policlinics to Microbiology Laboratuary between January 2014 and June 2015 were investigated. Rotavirus and adenovirus antigens were investigated in stool samples using the kit Rota-Adeno Card Test which uses a colored chromatographic method. Results:Rotavirus was detected in 129 of 1101 (11.7%) specimens and adenovirus was detected in 42 of 1254 (3.3%) specimens. Two specimens were positive for both of the viruses. Positivity for rotavirus and adenovirus was determined mostly in winter and spring and at 13 -24 months of age. There was no statistically significant difference between adenovirus and rotavirus antigen positivity regarding age and seasons (p>0.05). Conclusion: Investigation of rotavirus and adenovirus antigens in stool samples is important for epidemiological purposes and preventing the use of unnecessary antibiotics.Pam Med J 2017;(1):61-65

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Viral acute gastroenteritis: clinical and epidemiological features of co-infected patients

Cited by 27 publications

References 40 publications

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

Absence of Genetic Differences among G10P[11] Rotaviruses Associated with Asymptomatic and Symptomatic Neonatal Infections in Vellore, India

Investigation of the frequency of rotavirus and adenovirus in acute gastroenteritis cases

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