A survey on pulmonary pathogens and their antibiotic susceptibility among cystic fibrosis patients

Khanbabaee, Ghamartaj; Akbarizadeh, Majid Reza; Sayyari, Aliakbar; Ashayeri-Panah, Mitra; Abdollahgorji, Fatemeh; Sheibani, Kourosh; Rezaeig, Nima

doi:10.1590/s1413-86702012000200003

Cited by 7 publications

(12 citation statements)

References 23 publications

(34 reference statements)

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“…Comparing with the previous bacteriological survey published from our same center, the number of patients with CF colonized with Pseudomonas spp. increased during the last 6 years from 38.8 to 45.9% [21]. This highlights the importance of screening and eradicating P. aeruginosa strains.…”

Section: Discussionmentioning

confidence: 98%

Microbial contamination of home nebulizers in children with cystic fibrosis and clinical implication on the number of pulmonary exacerbations

et al. 2020

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Background: Early detection of pulmonary contamination in children with cystic fibrosis (CF) is essential since these children are vulnerable to Pseudomonas aeruginosa (P. aeruginosa) colonization. In Iran, home nebulization of antibiotics is a widespread practice in treatment for patients with CF and, to the best our knowledge, no bacteriological surveys have been conducted till date in this regard. Method: This observational, cross sectional study was conducted on 61 children with CF at Mofid Children's Hospital, Tehran, from September 2017 to march 2018. The swab sampling was performed from 61 home nebulizers used by children diagnosed with CF. Contemporaneous sputum sample or deep nasopharyngeal swab was taken from each patient for bacterial and fungal testing. Medical records of the patients were reviewed and the number of exacerbations were recorded over the last 12 months prior to the study enrollment. Results: The results of study showed that, 43 (70.5%) nebulizers were contaminated; 31 (50.8%) mouthpieces, 21 (34.4%) reservoirs, and 11 (18%) connecting tubes. The most common organism to be isolated was P. aeruginosa and was recovered from 19 (31%) nebulizers, 16 of them belonged to patients chronically colonized with P. aeruginosa. The remaining three had at least one positive sputum culture for P. aeruginosa in the past 1 year before the study. There was a significant increase in the number of CF exacerbations with an average number of exacerbation being 1.5 ± 1(SD) over last 12 months in children who had pathogenic organisms recovered from their home nebulizers compared with 0.4 ± 0.7(SD) exacerbations per year in whom non-pathogenic organisms were isolated from their nebulizers (P < 0.001). Conclusion: The majority of domiciliary nebulizers used by children with CF were contaminated with microorganisms indicating that the nebulizers may serve as potential reservoirs of pathogens for the patients' lung. Perpetuating colonization is a possible concern in the ones recently colonized with P. aeruginosa and, therefore, decontamination of nebulizer requires more attention to prevent ongoing infection. The negative impact of contamination of nebulizer on CF exacerbation requires serious attention and further investigations.

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Section: Discussionmentioning

confidence: 98%

Microbial contamination of home nebulizers in children with cystic fibrosis and clinical implication on the number of pulmonary exacerbations

et al. 2020

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“…The results of these three studies are comparable to the findings of the current study. In a study conducted in Tehran by Khanbabaee et al, P. aeruginosa was again found to be the most prevalent species isolated from 129 pediatric CF patients (20).…”

Section: Discussionmentioning

confidence: 98%

Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran

Azimi

Ghanaiee

Shirdust

et al. 2019

Arch Pediatr Infect Dis

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Background: Gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (CF). Objectives: The study aimed to conduct the molecular identification of Gram-negative bacterial flora causing pulmonary infection in children with CF. Methods: In this study, sputum samples were taken from 64 CF children undergoing treatment as outpatients or inpatients at a referral children's hospital in Tehran. The PCR technique was used to detect the presence of Gram-negative bacteria, namely Pseudomonas spp., Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia. Results: All samples were positive for 16srRNA. Pseudomonas spp. and A. baumannii were detected in 47% and 14% of the studied samples, respectively. Co-colonization by Pseudomonas spp. and A. baumannii was observed in three (5%) samples. Conclusions: According to this survey, Pseudomonas spp. were the most prevalent Gram-negative bacteria isolated from CF patients with pulmonary infection by molecular assays.

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“…The use of copper‐based chemicals and commercial antibiotics is a primary approach to manage newly emerging bacterial diseases (Kado, ; Osdaghi et al ., , ). Rifampicin is one of the most commonly used antibiotics in clinical environments in southeastern Iran to combat infections of cystic fibrosis patients suspected to be caused by Bcc (Khanbabaee et al ., ). Therefore, all the bacterial strains obtained in this study were evaluated for sensitivity to rifampicin and copper sulphate.…”

Section: Methodsmentioning

confidence: 97%

Pathogenicity and molecular phylogenetic analysis reveal a distinct position of the banana fingertip rot pathogen among the Burkholderia cenocepacia genomovars

et al. 2018

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Banana (Musa spp.) is one of the most widely cultivated subtropical fruits around the globe. Banana cultivation has been extensively increased in southeastern Iran over the last two decades. Recently, banana fruits possessing rotten and blackened fingertip symptoms were observed in Sistan-Baluchestan, Iran. Isolation and characterization of the causal agent showed that the pathogen belongs to the multifaceted bacterial species Burkholderia cenocepacia. Pathogenicity tests and host range assays showed that the strains were pathogenic on banana, as well as carrot, onion and potato. All the strains were resistant to 50 mg L À1 rifampicin and 200 mg L À1 copper sulphate. Phylogenetic analysis of 16S rRNA and recA gene sequences showed that the strains belong to two different genomovars of B. cenocepacia (III-A and III-B), which also include environmental and cystic fibrosis associated strains of the species. The results obtained from recA phylogeny were confirmed using multilocus sequence analysis (MLSA), although MLSA showed that the banana strains were clustered as a novel phylogroup among the members of both genomovars. Banana-pathogenic B. cenocepacia strains isolated in Iran were different from the strains isolated in Taiwan, as the 'B. cepacia epidemic strain marker' reported in the Taiwanese strains was absent from Iranian strains. To the authors' knowledge, this is the first MLSA-based study on the banana-pathogenic strains of B. cenocepacia. However, further in-depth molecular studies are needed to decipher the relationships between the banana fingertip rot pathogen and the clinical strains of B. cenocepacia.

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A survey on pulmonary pathogens and their antibiotic susceptibility among cystic fibrosis patients

Cited by 7 publications

References 23 publications

Microbial contamination of home nebulizers in children with cystic fibrosis and clinical implication on the number of pulmonary exacerbations

Microbial contamination of home nebulizers in children with cystic fibrosis and clinical implication on the number of pulmonary exacerbations

Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran

Pathogenicity and molecular phylogenetic analysis reveal a distinct position of the banana fingertip rot pathogen among the Burkholderia cenocepacia genomovars

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