Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction

Khosravi, Azar Dokht; Goodarzi, Hamed; Alavi, Seyed Mohammad

doi:10.1590/s1413-86702012000100010

Cited by 7 publications

(8 citation statements)

References 11 publications

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“…Molecular biological detection techniques may be used to detect the detailed molecular characteristics of katG and inhA gene mutations, which may provide rapid early diagnosis for the treatment of drug-resistant TB. The mutation spectrum and mutation rates of katG and inhA have regional differences, but katG315 and inhA-15 are the most common mutation sites (17,18). Therefore, the detection of mutations at these two sites has potential application in determination of INH resistance.…”

Section: Introductionmentioning

confidence: 99%

Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid

Tang

Wang

Shen

et al. 2017

Experimental and Therapeutic Medicine

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The objective of the present study was to evaluate the potential development of DNA microarray chips to detect rifampicin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB), using samples from clinical tuberculosis (TB) patients in Soochow City, China. The sputum samples of 42 patients with TB in the Affiliated Hospital of Infectious Diseases of Soochow University (Soochow, China) were collected. The conventional Lowenstein-Jensen culture medium (Gold Standard) was used to assess drug sensitivity using the absolute concentration method. GeeDom MTB drug detection kits were also used to create a DNA microarray chip and examine the RFP-resistance associated gene mutation points rpoB-RRDR 511, 513, 516, 526, 531 and 533, and the INH-resistance associated gene mutation points katG315 and inhA-15 of the sputum samples. Compared with the results from the absolute concentration method, the susceptibility and specificity of RFP sensitivity detected by the DNA microarray chip were 92.8 and 93.8%, respectively. The susceptibility and specificity of INH sensitivity detected were 66.7 and 81%, respectively. The rpoB-RRDR 526, 531 mutations were the primary causes of MTB RFP resistance and the katG315 mutation was the primary cause of INH resistance. The detection of rpoB and katG gene mutation points by a DNA microarray chip may be used as a rapid, accurate and bulk clinical detection method for RFP and INH resistance in MTB. This is very valuable for the control of TB epidemics.

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Section: Introductionmentioning

confidence: 99%

Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid

Tang

Wang

Shen

et al. 2017

Experimental and Therapeutic Medicine

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“…In Farazi A et al .,[ 16 ] isoniazid resistance in new cases was 2.9% and 2.6% in total, and rifampin resistance in new cases was 1% and total 1.7%. In Khosravi AD et al .,[ 17 ] resistance to rifampin and isoniazid was reported to be 15% and 13.7%, respectively. In Tavanaee Sani A et al .,[ 18 ] resistance to isoniazid was 7%, resistance to rifampin 7% and resistance to both 4%.…”

Section: Discussionmentioning

confidence: 99%

“…[ 13 ] Isoniazid and rifampin resistance in other study seem are higher than us because of the higher patient group and cases with previous history of TB. [ 10 17 19 ]…”

Section: Discussionmentioning

confidence: 99%

“…In our study mean age: 38.78 years was close to Nyagosya Range[ 9 ] (34.22 years), Akaninyene Out[ 8 ] (34. years), Kelemework Adane[ 13 ] (36.8 years), Park[ 19 ] (31.1 years), Abhishek[ 17 ] and Pourhaji et al . [ 15 ] but was different to Tavanaee[ 18 ] and Velayati,[ 20 ] this is due to the difference in age between the youngest and oldest.…”

Section: Discussionmentioning

confidence: 99%

“…There were no significant association between smoking and drug resistance is similar to Tavanaee Sani et al .,[ 18 ] Abhishek Agarwal et al . [ 17 ] and this factor was not evaluated in other studies.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the frequency of resistance to 2 drugs (Isoniazid and Rifampin) by molecular investigation and it's risk factors in new cases of smear positive pulmonary tuberculosis in health centers under the cover of Jundishapur University of Medical Sciences in 2017

Salmanzadeh

Karamian

Alavi

et al. 2020

J Family Med Prim Care

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Introduction: Despite the great efforts to control tuberculosis (TB), the disease is still one of the major health challenges throughout the world. The basic treatment for TB is drug therapy. Currently, the main anti-tuberculosis drugs with major use in the treatment and control of the disease are isoniazid, rifampin, pyrazinamide, ethambutol, and streptomycin. One of the serious crises in controlling TB epidemic is diagnosis and treatment of patients with Multidrug Resistant Tuberculosis (MDR-TB MDR). The purpose of the study was to examine and evaluate the resistance of mycobacterium TB strains isolated from specimens of newly diagnosed smear positive pulmonary TB to isoniazid and rifampin using molecular methods and their risk factors. Methods: Sputum samples of newly diagnosed smear positive pulmonary TB patients were prepared, collected, and sent to Reference Laboratory in Ahvaz. DNA of mycobacterium tuberculosis was prepared from the samples using Qiagen kit according to the instructions of the manufacturing company. Isoniazid resistance was evaluated using specific primers for inhA and KatG genes. Rifampin resistance was evaluated using MAS-PCR method with three specific alleles of rpobB codons and codons 516, 526 and 531. Results: Mycobacterium tuberculosis resistance to Isoniazid was 7.3%, to Rifampin 5.5% and to both drugs 1.8%. In our study, there were no association between drug resistance and gender, age, prison history, smoking, drug use, underlying disease, occupation, and HIV. Conclusion: According our findings that include prevalence of 7.3% Isoniazide resistance, 5.5% Rifampin resistance and 1.8% to both drugs, evaluating all newly diagnosed patients for resistance to standard anti-tuberculosis treatment seems rational.

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Structural analyses and classification of novel isoniazid resistance coupled mutational landscapes inMycobacterium tuberculosis: a combined molecular docking and MD simulation study

Shahbaaz

Qari

Abdellattif

et al. 2020

Journal of Biomolecular Structure and Dynamics

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Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction

Cited by 7 publications

References 11 publications

Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid

Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid

Evaluation of the frequency of resistance to 2 drugs (Isoniazid and Rifampin) by molecular investigation and it's risk factors in new cases of smear positive pulmonary tuberculosis in health centers under the cover of Jundishapur University of Medical Sciences in 2017

Structural analyses and classification of novel isoniazid resistance coupled mutational landscapes inMycobacterium tuberculosis: a combined molecular docking and MD simulation study

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