Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa

Perez, Leandro Reus Rodrigues; Barth, Afonso Luís

doi:10.1590/s1413-86702011000400001

Cited by 8 publications

(11 citation statements)

References 10 publications

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“…According to the intensity of the violet color of the stained biofilm, the biofilm-forming ability of different P. aeruginosa isolates was classified as strongly adherent, moderately adherent, weakly adherent, or nonadherent. In this work, P. aeruginosa strain PAO1 and strain PA14 were used as references for strong and weak adherent strains, respectively, where the biofilmproducing abilities for these standard strains were confirmed in previous reports [27][28][29].…”

Section: Qualitative Detection Of Biofilm With the Tube Methodsupporting

confidence: 64%

“…erefore, we selected these antibiotics and investigated their inhibitory effects on the biofilm formation ability of P. aeruginosa. A previous study revealed that isolates with a higher ability to produce biofilm and had a tendency to produce higher rates of resistance to many antimicrobial agents [28]. Antibiotics at concentrations higher than their MICs do not induce killing effects on bacteria in biofilm communities [16,40].…”

Section: Biofilm Reduction (%)mentioning

confidence: 98%

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Effects of Lysozyme, Proteinase K, and Cephalosporins on Biofilm Formation by Clinical Isolates of Pseudomonas aeruginosa

Eladawy

El-Mowafy

El‐Sokkary

et al. 2020

Interdisciplinary Perspectives on Infectious Diseases

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Pseudomonas aeruginosa is an opportunistic pathogen that can form biofilms, which confer resistance to immune clearance and antibacterial treatment. Therefore, effective strategies to prevent biofilm formation are warranted. Here, 103 P. aeruginosa clinical isolates were quantitatively screened for biofilm formation ability via the tissue culture plate method. The effects of lysozyme (hydrolytic enzyme) and proteinase K (protease) on biofilm formation were evaluated at different concentrations. Lysozyme (30 μg/mL), but not proteinase K, significantly inhibited biofilm formation (19% inhibition). Treatment of 24-hour-old biofilms of P. aeruginosa isolates with 50 times the minimum inhibitory concentrations (MICs) of ceftazidime and cefepime significantly decreased the biofilm mass by 32.8% and 44%, respectively. Moreover, the exposure of 24-hour-old biofilms of P. aeruginosa isolates to lysozyme (30 μg/mL) and 50 times MICs of ceftazidime or cefepime resulted in a significant reduction in biofilm mass as compared with the exposure to lysozyme or either antibacterial agent alone. The best antibiofilm effect (49.3%) was observed with the combination of lysozyme (30 μg/mL) and 50 times MIC of cefepime. The promising antibiofilm activity observed after treatment with 50 times MIC of ceftazidime or cefepime alone or in combination with lysozyme (30 μg/mL) is indicative of a novel strategy to eradicate pseudomonal biofilms in intravascular devices and contact lenses.

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Section: Qualitative Detection Of Biofilm With the Tube Methodsupporting

confidence: 64%

Section: Biofilm Reduction (%)mentioning

confidence: 98%

Effects of Lysozyme, Proteinase K, and Cephalosporins on Biofilm Formation by Clinical Isolates of Pseudomonas aeruginosa

Eladawy

El-Mowafy

El‐Sokkary

et al. 2020

Interdisciplinary Perspectives on Infectious Diseases

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“…OD the isolate between 2X ODc and 4X ODc is considered moderate biofilm and OD the isolate > 4X ODc is considered strong biofilm producing strain. 1 …”

Section: Identification Of Strainsmentioning

confidence: 99%

“…1 This bacterium is the most frequently isolated Gram-negative organism in bloodstream, wound infections, pneumonia, intra-abdominal and urogenital sepsis. This is a serious problem, also infecting immunocompromised patients with cystic fibrosis, severe burns, cancer, AIDS, etc.…”

Section: Introductionmentioning

confidence: 99%

“…3 It is well recognized that biofilm mode of growth can promote resistance to antimicrobial agents, and its occurrence during the infectious process has been considered a limiting factor for therapeutic success. 1 Biofilm is a structured community of bacterial cells adherent to an inert or living surface and/or embedded in a selfproduced extracellular polymeric substances' (EPS) matrix. The bacteria within the biofilms are protected from physical, chemical and biological stresses, including antimicrobial agents, antibodies and the antimicrobial products of phagocytic cells.…”

Section: Introductionmentioning

confidence: 99%

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Bacteriophage-antibiotic synergism to control planktonic and biofilm producing clinical isolates of Pseudomonas aeruginosa

Nouraldin

Baddour²,

Harfoush³

et al. 2016

Alexandria Journal of Medicine

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Introduction: Pseudomonas aeruginosa (P. aeruginosa) is a highly resistant opportunistic pathogen and is capable of forming biofilms on medical devices. Bacterial biofilms, which are micro-colonies encased in extracellular polysaccharide material are so difficult to be treated by conventional antibiotics. During the last decade, P. aeruginosa phages have been extensively examined as an alternative to antimicrobial agents. The aim of the study was to assess bacteriophageantibiotic combination on planktonic and biofilm states of P. aeruginosa isolates. Materials: In this study, we isolated 6 lytic phages, from hospital effluents, they were tested against 50 P. aeruginosa strains, isolated from different clinical specimens delivered to the Diagnostic Microbiology Laboratories, Faculty of Medicine, Alexandria University. Results: Out of the 50 isolates, 15 were susceptible to these phages. So the biofilm forming capacity of these 15 isolates was investigated. The results showed that 14 isolates (93.33%) produced detectable biofilm. The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) assays were used to evaluate the antibiotic sensitivity patterns of these P. aeruginosa isolates in their planktonic and biofilm phases to amikacin and meropenem. Also, the effects of phage on the planktonic and biofilm states of isolates at different multiplicities of infections (MOI) were tested. On the planktonic state, the amikacin-phage combination showed synergistic effect (P = 0.001), and the meropenem-phage combination showed synergistic effect (P = 0.003). On the biofilm state, the amikacin-phage combination showed biofilm eradication Please cite this article in press as: Nouraldin AAM et al. Bacteriophage-antibiotic synergism to control planktonic and biofilm producing clinical isolates of Pseudomonas aeruginosa, Alex J Med (2015), http://dx.doi.org/10.1016/j.ajme.2015.05.002in 50% of the isolates (P = 0.003). On the other hand, the meropenem-phage combination showed biofilm eradication in 14.3% of the strains. Conclusion: The combination of phage and antibiotics could have potentially more benefits on P. aeruginosa planktonic and biofilm states than just using phages or antibiotics alone.

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Essential oils against bacterial isolates from cystic fibrosis patients by means of antimicrobial and unsupervised machine learning approaches

Ragno

Papa²,

Patsilinakos

et al. 2020

Sci Rep

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Recurrent and chronic respiratory tract infections in cystic fibrosis (CF) patients result in progressive lung damage and represent the primary cause of morbidity and mortality. Staphylococcus aureus (S. aureus) is one of the earliest bacteria in CF infants and children. Starting from early adolescence, patients become chronically infected with Gram-negative non-fermenting bacteria, and Pseudomonas aeruginosa (P. aeruginosa) is the most relevant and recurring. Intensive use of antimicrobial drugs to fight lung infections inevitably leads to the onset of antibiotic resistant bacterial strains. New antimicrobial compounds should be identified to overcome antibiotic resistance in these patients. Recently interesting data were reported in literature on the use of natural derived compounds that inhibited in vitro S. aureus and P. aeruginosa bacterial growth. Essential oils, among these, seemed to be the most promising. In this work is reported an extensive study on 61 essential oils (EOs) against a panel of 40 clinical strains isolated from CF patients. To reduce the in vitro procedure and render the investigation as convergent as possible, machine learning clusterization algorithms were firstly applied to pickup a fewer number of representative strains among the panel of 40. This approach allowed us to easily identify three EOs able to strongly inhibit bacterial growth of all bacterial strains. Interestingly, the EOs antibacterial activity is completely unrelated to the antibiotic resistance profile of each strain. Taking into account the results obtained, a clinical use of EOs could be suggested. Cystic fibrosis (CF), one of the most common lethal genetic disorders in Caucasian population, is inherited as an autosomal recessive disease and affects 70.000 persons worldwide (Cystic Fibrosis Foundation, CFF). The defective gene, identified in 1989, is the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) that is carried by 4% of persons (among Caucasians). Since CFTR encodes for a chloride channel of the epithelial cell surface, CF patients manifest a variety of multi-organ problems due to the alteration of sodium and chloride secretion across cell membranes and the subsequent luminal dehydration 1. The impairment of mucociliary clearance, which should remove all microbes entering the airways, leads to the production of a thick and dehydrated mucus in the CF lung, which promotes the airway chronic bacterial colonization 2. The microbiology of CF respiratory tract is peculiar. In the early stage of life, it is characterized by the prevalence of the Gram-positive bacterium Staphylococcus aureus (S. aureus). Overall, in 2017 more than half of affected individuals had at least one culture positive for methicillin sensitive S. aureus (MSSA). The highest prevalence of methicillin resistant S. aureus (MRSA) occurs in individuals between the ages of 10 and 30, while MSSA reaches the peak among patients younger than 10 (Cystic Fibrosis Foundation. 2017. Patient Registry Annual

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Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa

Cited by 8 publications

References 10 publications

Effects of Lysozyme, Proteinase K, and Cephalosporins on Biofilm Formation by Clinical Isolates of Pseudomonas aeruginosa

Effects of Lysozyme, Proteinase K, and Cephalosporins on Biofilm Formation by Clinical Isolates of Pseudomonas aeruginosa

Bacteriophage-antibiotic synergism to control planktonic and biofilm producing clinical isolates of Pseudomonas aeruginosa

Essential oils against bacterial isolates from cystic fibrosis patients by means of antimicrobial and unsupervised machine learning approaches

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