Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil

Wollheim, Cláudia; Guerra, Ivani Maria F; Conte, Vania D; Hoffman, Sheila P; Schreiner, Fernando J; Delamare, Ana Paula L; Barth, Afonso Luís; Echeverrigaray, Sérgio; Costa, Sérgio Olavo P da

doi:10.1590/s1413-86702011000200008

Cited by 18 publications

(20 citation statements)

References 25 publications

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“…Similar results were reported by other investigators [5]. Additionally, urine samples were the second major source for ESBL-producing isolates.…”

Section: Discussionsupporting

confidence: 91%

“…Our results showed that the majority of ESBL isolates (189; 80%) were obtained from inpatients, indicating that ESBLassociated infections in our population remain essentially nosocomial in nature and their dissemination to the community is not very significant. This finding is consistent with other regional and international data [32,35,5]. However, unless strict infection control measures are enforced, there is a high likelihood that these ESBL producers can be trafficked from the hospital into the community.…”

Section: Discussionsupporting

confidence: 91%

“…Similarly, in a study conducted in Brazil, 74.3% of ESBL-producing K. pneumonia isolates selected for conjugation experiments transferred ESBL genes by conjugation. The obtained transconjugants exhibited concomitant decreased aminoglycoside susceptibility [5]. This observation highlights the necessity of rapid and efficient infection control procedures.…”

Section: Discussionmentioning

confidence: 75%

“…Since the introduction of broad-spectrum cephalosporins into clinical practice in the early 1980s, the selective pressure of the use and overuse of antibiotics has resulted in the emergence and rapid development of resistance to expandedspectrum beta-lactam antibiotics [1,2]. Numerous outbreaks of infection with organisms producing extended-spectrum beta-lactamases (ESBLs) have been observed in many countries throughout the world [2,3], and these organisms have achieved notoriety for causing nosocomial infections that lead to prolonged hospital stay, increased morbidity and mortality, and consequently increased health-care associated costs [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping

Hassan

Alkharsah

Alzahrani

et al. 2013

J Infect Dev Ctries

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Introduction: Few reports about the prevalence and genetic basis of extended spectrum beta-lactamases (ESBLs) are available from Saudi Arabia. We sought to determine the prevalence of ESBL-producing Enterobacteriaceae in a university hospital in eastern Saudi Arabia and to characterize the ESBLs produced by these isolates at the molecular level. Methodology: All clinical isolates of Escherichia coli, Klebsiella spp., and Proteus spp. collected over two years were evaluated for susceptibility to a panel of antimicrobials and were analyzed for the ESBL phenotype using screening and confirmatory tests. ESBL-positive isolates were then screened for the presence of genes encoding CTX-M, SHV, and TEM beta-lactamases by PCR. Results and conclusions: The overall prevalence of ESBL-producing isolates was 4.8% (253/5256). Most isolates (80%) were from the inpatient department. The ESBL phenotype was more frequently detected in K. pneumonia. CTX-M genes were the most prevalent ESBL genes, detected in 82% of the studied isolates. The ESBL producers demonstrated a high multidrug resistance rate (96.6%). In transconjugation assay, the same ESBL gene pattern was transmitted from 29.7% of K. pneumoniae donors to the recipient strain, and the latter exhibited concomitant decreased aminoglycosides and co-trimoxazole susceptibility. We observed the presence of ESBL screenpositive but confirmatory-negative isolates (8.9%). Phenotypic tests for the production of AmpC β-lactamase tested positive in 52% of these isolates. Further studies are needed for appropriate detection of concomitant ESBL and AmpC enzyme production among such isolates. Continued surveillance and judicious antibiotic usage together with the implementation of efficient infection control measures are absolutely required.

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“…Similar results were reported by other investigators [5]. Additionally, urine samples were the second major source for ESBL-producing isolates.…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping

Hassan

Alkharsah

Alzahrani

et al. 2013

J Infect Dev Ctries

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“…The prevalence of ESBL among community-acquired UPEC strains observed in this work was higher than observed among strains isolated in the southern (0.5%) and southeastern (1.5%) regions of Brazil [12,13] and lower than those isolated in the northern region of the country (7.6%). [14].…”

Section: Resultscontrasting

confidence: 75%

Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil

Gonçalves

Martins-Júnior

Melo

et al. 2016

Journal of Global Antimicrobial Resistance

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Evaluation of disinfection efficacy of ozone and chlorinated disinfectant against the biofilm of Klebsiella michiganensis and Pseudomonas aeruginosa

Saha

Atwain

et al. 2014

Ann Microbiol

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Household microorganisms mostly reside in the form of biofilms on wet surfaces in the bathroom and kitchen areas. Microorganisms constituting biofilm communities are less susceptible than their planktonic counterparts to antimicrobial agents and are thus difficult to control. Traditionally, chemical disinfectants have been used as cleaners in the household environment. Recently, incorporation of ozone as a microbial control agent in the consumer product industry has been initiated as an alternative method of disinfection. In this study, antimicrobial efficacy of ozone and a commercial chlorinated disinfectant was evaluated against the biofilm of a commonly occurring Pseudomonas aeruginosa and Klebsiella michiganensis, a newly described species recovered from toothbrush holder. Single species biofilm was grown on borosilicate glass and polycarbonate coupons using the Centers for Disease Control and Prevention (CDC) biofilm reactor. This is the first study demonstrating the biofilm forming capability of K. michiganensis under laboratory conditions using the CDC biofilm reactor. Planktonic cells and mature biofilms were exposed to ozonated water for 2 and 4 min and chlorinated cleaner for 2 min and 10 min. Based on the ozone stability study, the exposure experiments were carried out in two different ways. The type 1 treatment was carried out according to ASTM method E2871-12, with 5 mL of ozonated water as disinfectant in a 50 mL conical centrifuge tube; whereas in type 2 treatment, 14.0 mL of ozonated water was used to avoid any headspace in the capped culture tube. In biofilms, type 1 ozone treatment demonstrated an average log reduction of 0.88 (±0.13) and 0.12 (±0.01) for K. michiganensis and P. aeruginosa, respectively, in 2 min. Similarly, for type 2 treatment, the values were 1.56 (±0.58) and 0.27 (±0.08). In planktonic cells, log reduction was 2.61 (±0.11) in P. aeruginosa and>4.0 in K. michiganensis, within 2 min. Chlorinated cleaner demonstrated > 4.0 log reduction in all cases. The disinfection efficacy of ozone type 1 treatment varied between bacterial species (p<0.001) and exposure times (p<0.04), but not the coupon types (p>0.95). Within biofilms, log reduction varied significantly between ozone and chorine treatments (p<0.001). Significant differences in log reduction were also observed between planktonic cells and biofilms (p<0.001), target organisms (p<0.05) and the types of ozone treatment (p<0.05, for K michiganensis only). From the results, it can be concluded that K. michiganensis was more susceptible to the disinfectants compared to P. aeruginosa. Within a shorter contact time, commercial chlorinated cleaner was more effective against both biofilms and planktonic cells than ozonated water.

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Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil

Cited by 18 publications

References 25 publications

Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping

Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping

Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil

Evaluation of disinfection efficacy of ozone and chlorinated disinfectant against the biofilm of Klebsiella michiganensis and Pseudomonas aeruginosa

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