Health care workers humoral immune response against GLcB, MPT51 and HSPX from Mycobacterium tuberculosis

Reis, Marcio R. C.; Rabahi, Marcelo Fouad; Kipnis, André; Junqueira-Kipnis, Ana Paula

doi:10.1590/s1413-86702009000600006

Cited by 9 publications

(10 citation statements)

References 33 publications

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“…Of these, an abstract of a full article in Portuguese language was in English and reported prevalence of LTBI among HCWs, and was included in both qualitative and quantitative synthesis [ 22 ]. Five articles and two abstracts were excluded for probable duplication [ 23 – 29 ]. One abstract that did not include the number of TST positives was excluded [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Latent Tuberculosis among Health Care Workers in High Burden Countries: A Systematic Review and Meta-Analysis

2016

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BackgroundTuberculosis is one of the leading causes of death worldwide. Twenty-two high burden countries contributed to the majority of worldwide tuberculosis cases in 2015. Health care workers are at high risk of acquiring tuberculosis through occupational exposure.ObjectiveTo estimate the prevalence of latent tuberculosis infection (LTBI) among health care workers in high burden countries.MethodsDatabases including MEDLINE (Ovid), EMBASE (Ovid), CINAHL (Ovid) and ISI Web of Science (Thompson-Reuters), and grey literature were searched for English language records on relevant medical subject headings (MeSH) terms of LTBI and health care providers. Literature was systematically reviewed using EPPI-Reviewer4 software. Prevalence and incidence of LTBI and 95% confidence intervals (CI) were reported. Pooled prevalence of LTBI and 95% CI were calculated using random-effects meta-analysis models and heterogeneity was assessed using I2 statistics. Sub-group analysis was conducted to assess the cause of heterogeneity.ResultsA total of 990 records were identified. Of those, 18 studies from only 7 high burden countries representing 10,078 subjects were included. Tuberculin skin test results were available for 9,545 participants. The pooled prevalence of LTBI was 47% (95% CI 34% to 60%, I2 = 99.6%). In subgroup analyses according to the country of the study, the pooled prevalence of LTBI was lowest in Brazil (37%) and highest in South Africa (64%). The pooled prevalence of LTBI among medical and nursing students was 26% (95% CI 6% to 46%, I2 = 99.3%) while the prevalence among all types of health care workers was 57% (95% CI 44% to 70%, I2 = 99.1%). Incidence of LTBI was available for health care workers in four countries. The cumulative incidence ranged from 2.8% in Brazilian medical students to 38% among all types of health care workers in South Africa.ConclusionThe findings of this study suggest that there is a high burden of LTBI among health care workers in high burden countries. Adequate infection control measures are warranted to prevent and control transmission in health care settings.

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Section: Resultsmentioning

confidence: 99%

Prevalence of Latent Tuberculosis among Health Care Workers in High Burden Countries: A Systematic Review and Meta-Analysis

2016

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“…To our knowledge, this work is the first study to evaluate cell subtypes by their specific immune responses to Mtb antigens in the active (GlcB) and latent (HspX and GlcB) phases of TB. These antigens were specifically recognised by Abs and T cells from active and LTBI patients who had previously received BCG vaccinations in endemic areas and who were thus important for this study ( Rabahi et al 2007 , Reis et al 2009 ). Therefore, combining one antigen expressed by Mtb during the latent phase of infection with another more ubiquitous antigen may improve the diagnosis of LTBI individuals.…”

Section: Discussionmentioning

confidence: 99%

“…2006 , Hougardy et al 2007 , Araújo-Filho et al 2008 , Scriba et al 2008 , Cruz et al 2009 ). Many antigens, such as heat shock protein X (HspX) antigens, can be used to analyse the overall immune responses of LTBI individuals ( Tavares et al 2006 , Rabahi et al 2007 , Reis et al 2009 ); however, these antigens have not been tested for specific cellular responses. Thus, the objectives of this work were to analyse the specific immune responses of Th1, Th17 and Treg cells from RA patients to malate synthase (GlcB) and HspX from Mtb and to evaluate the use of these responses for identifying LTBI RA patients.…”

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confidence: 99%

The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients

Silva

Tannus-Silva

Rabahi

et al. 2014

Mem. Inst. Oswaldo Cruz

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Rheumatoid arthritis (RA) is an autoimmune disease characterised by the destruction of articular cartilage and bone damage. The chronic treatment of RA patients causes a higher susceptibility to infectious diseases such as tuberculosis (TB); one-third of the world’s population is latently infected (LTBI) with Mycobacterium tuberculosis (Mtb). The tuberculin skin test is used to identify individuals LTBI, but many studies have shown that this test is not suitable for RA patients. The goal of this work was to test the specific cellular immune responses to the Mtb malate synthase (GlcB) and heat shock protein X (HspX) antigens of RA patients and to correlate those responses with LTBI status. The T-helper (Th)1, Th17 and Treg-specific immune responses to the GlcB and HspX Mtb antigens were analysed in RA patients candidates for tumour necrosis factor-α blocker treatment. Our results demonstrated that LTBI RA patients had Th1-specific immune responses to GlcB and HspX. Patients were followed up over two years and 14.3% developed active TB. After the development of active TB, RA patients had increased numbers of Th17 and Treg cells, similar to TB patients. These results demonstrate that a GlcB and HspX antigen assay can be used as a diagnostic test to identify LTBI RA patients.

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“…Studies that analyzed the humoral and cellular immune response to MPT51 verified discrimination between patients with active TB from healthy individuals [36]. …”

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confidence: 99%

“…Moreover, HspX epitopes can be recognized by CD4 + and CD8 + T cells from active TB patients [36, 42]. …”

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confidence: 99%

Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

Silva

Cavalcanti

Oliveira

et al. 2014

The Journal of Veterinary Medical Science

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This study aimed to evaluate the immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the CMX fusion protein composed of immunodominant epitopes Ag85C, MPT51 and HspX of Mycobacterium tuberculosis, which are important mycobacteria virulence factors. A group of Nelore heifers that were 10 to 12 months of age and negative for the tuberculin skin test (TST) were immunized with four doses of the recombinant vaccine mc2-CMX (M. smegmatis-Ag85C-MPT51-HspX) during a period of one year. Before each immunization, blood was collected to obtain sera for antibody analysis. Serological analysis demonstrated that mc2-CMX was able to induce a humoral response with increased levels of specific IgG antibodies against CMX, despite minimum antibody levels being detected for individual Ag85C, MPT51 or HspX recombinant antigens. However, there was no significant increase in specific CD4+ IFN-γ-positive T cells. Lymphadenomegaly was observed in superficial cervical lymph nodes adjacent to the site of vaccination among mc2-CMX-vaccinated bovines, and the histopathological analysis demonstrated follicular hyperplasia without inflammatory infiltrate or granuloma formation. Animals remained negative for the TST until the end of the experiments, showing no cross-reactivity with the recombinant vaccine and tuberculin proteins. We discuss the potential of mc2-CMX to induce an immune response in cattle.

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Health care workers humoral immune response against GLcB, MPT51 and HSPX from Mycobacterium tuberculosis

Cited by 9 publications

References 33 publications

Prevalence of Latent Tuberculosis among Health Care Workers in High Burden Countries: A Systematic Review and Meta-Analysis

Prevalence of Latent Tuberculosis among Health Care Workers in High Burden Countries: A Systematic Review and Meta-Analysis

The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients

Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

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