Prevalence and risk factors for Staphylococcus aureus in health care workers at a University Hospital of Recife-PE

Abstract

Staphylococcus aureus is the main human pathogen that colonizes individuals in general population. The objective of the study was evaluate the epidemiological and sensitivity profile of S. aureus lineage, isolated in health care workers (HCW) of a University Hospital in Pernambuco state, Brazil. Biological samples of hands and nasal cavities were sown in agar sheep blood. Colonies under suspicion of being S. aureus were identified using Gram staining, catalase test and coagulase, mannitol-salty agar fermentation and DNAse agar. The resistance to mupirocin was analyzed through the Kirby Bauer technique. In relation to methicillin and vancomycin the determination was by the minimum inhibitory concentration method (E-test). From the 202 HCW evaluated, 52 were colonized by S. aureus (25,7%). The factors associated to the colonization by S. aureus were: age-group, professional category, use of individual protection equipments (frequency and numbers). All S. aureus isolate lineages were sensitive to mupirocin and vancomycin, and three of them were identified as methicillin-resistant. The prevalence of MSSA and MRSA among HCW was considered low and was below the results described in the literature. The isolate S. aureus lineages have shown low resistance profile.

The occurrence and dissemination of methicillin and vancomycin-resistant Staphylococcus in samples from patients and health professionals of a university hospital in Recife, State of Pernambuco, Brazil

“…The age group of MRSA occurrence among health professionals was the same as that described in other studies conducted in the same hospital in previous years 8,10,29 . The frequency of MRSA infection was highest in the 20-to 28-year-old age group.…”

Análise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina

“…podem produzir uma série de fatores de virulência que contribuem para que a bactéria invada as defesas fagocíticas do hospedeiro, facilite a sua aderência às células epiteliais e a colonização no tecido, favorecendo a sua persistência extracelular e garantindo assim êxito em sua instalação e manutenção nos tecidos do hospedeiro (Coelho et al 2011, Silva et al 2007). Entre estes fatores está a produção de um mucopolissacarídeo extracelular ("slime"), que parece ajudar na aderência e colonização do microrganismo ao epitélio glandular mamário.…”

Colonização bacteriana e resistência antimicrobiana em trabalhadores de saúde: revisão integrativa

“…The studies were published in 2008, (22) 2009, (14,15) 2010, (25) 2011, (23) 2012, (21) 2013, (12,13,16) 2014, (14,15,18) 2016, (20) 2017, (24) all in English. (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) In terms of level of evidence, 13 articles were cross-sectional, observational studies (level of evidence 6), (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) whereas one was a cohort study (level of evidence 4).…”

“…(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) In terms of level of evidence, 13 articles were cross-sectional, observational studies (level of evidence 6), (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) whereas one was a cohort study (level of evidence 4). (25) The studies were conducted in the following locations: USA, (12) Iran, (23,13) Brazil, (14,16,17,19,22) Ethiopia, (15) Sri Lanka, (18) Switzerland, (20) Libya,…”