Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil

Abstract

Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and nonpathogenic strains.

IDENTIFICATION AND TYPING OF Haemophilus influenzae IN IRAQI CHILDREN DIAGNOSED WITH MENINGITIS

“…Based on serotyping results, all isolates belonged to serotype b. These results were expected and as stated by shoma et al [13] and Lancellotti et al [31] that other serotypes not detected in CSF of children less than five years of age, suggesting that H. influenzae type b is the primary cause of invasive infections such as meningitis.…”

Whole-genome analysis of haemophilus influenzae invasive strains isolated from Campinas state University hospital. An epidemiological approach 2012 - 2019 and ancestor strains

“…All strains were isolated from blood cultures except one Hi2015-6 isolated from cerebrospinal fluid. Hi38 and Hi45 strains had been characterized by Lancellotti et al 2008 6 and also isolates from the same hospital in 1998. The bacterial strains were grown in chocolate agar plates or BHI supplement with NAD (4 µg/ mL) and hemin (10 µg/ mL) (Kilian, 1976 7 ) and incubated at 37°C with 5% CO 2 .…”

“…The bacterial strains were grown in chocolate agar plates or BHI supplement with NAD (4 µg/ mL) and hemin (10 µg/ mL) (Kilian, 1976 7 ) and incubated at 37°C with 5% CO 2 . 6 …”

Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever

“…Recombinant DNA protocols and transformation were performed as described previously (Hollanda and others; Lancellotti and others 2008 ). The oligonucleotides used are listed in Table 2 .…”