Type IV SCCmec found in decade old Brazilian MRSA isolates

Reinert, Cristina; McCulloch, John Anthony; Watanabe, Shinya; Ito, Teruyo; Hiramatsu, Kazumasa; Mamizuka, Elsa Masae

doi:10.1590/s1413-86702008000300010

Cited by 16 publications

(11 citation statements)

References 28 publications

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“…ST88 was the dominant clone among PVLpositive isolates. ST88 with PVL-positive isolates had been reported in Sweden, Brazil, Italy and Asia (Afroz et al, 2008;Fang et al, 2008;Reinert et al, 2008;Stefani et al, 2009). In China, this clone was the predominant lineages of PVL-positive clones and correlated to skin and soft tissue infection (Yao et al, 2010).…”

Section: Mlst-ccmentioning

confidence: 82%

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

Ma¹,

Chen²,

Zhou³

et al. 2011

Afr. J. Microbiol. Res.

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The aim of this study is to investigate the genetic backgrounds of methicillin susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA) and heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) isolates isolated from clinical specimens of the patients with verified infections in a Chinese teaching hospital. Macro E test (MET) was used to detect hVISA and multilocus sequence typing (MLST) was used to determine the STs of the selected isolates. The genotypes of SCCmec were determined by multiplex polymerase chain reaction (PCR) in MRSA isolates. Panton-valentine leucocidin (PVL) genes were also detected by PCR. Among 273 S. aureus isolates, hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA), community-associated methicillin-resistant S. aureus (CA-MRSA), hospital-acquired methicillin susceptible Staphylococcus aureus (HA-MSSA) and community-associated methicillin susceptible Staphylococcus aureus (CA-MSSA) isolates accounted for 55.6, 1.5, 36.3, and 6.6%, respectively. Nine isolates were confirmed as hVISA by MET. Among 60 HA-MRSA isolates, ST239-MRSA-III was the most prevalent clone accounting for 51.7%, followed by ST5-MRSA-II clone. Fifty percentage and 22.2% of CA-MSSA isolates were found to be ST121 and ST88. ST239-MRSA-III was the predominant clone in hVISA isolates. However, no predominant ST type was found in HA-MSSA isolates. Of 9 PVL-positive strains, ST88 was the most prevalent ST (50.0%; 4/8), followed by ST121 (33.3%; 3/9), ST5 (4.5%; 1/22) and ST239 (2.6%; 1/39). In conclusion, ST239-III was the major pandemic clone in hVISA and HA-MRSA and spread in China. ST5-emerging rapidly in China had remained stable viability.

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Section: Mlst-ccmentioning

confidence: 82%

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

Ma¹,

Chen²,

Zhou³

et al. 2011

Afr. J. Microbiol. Res.

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“…The patient remained hospitalized for 50 days and was discharged after treatment with clindamycin and gentamicin (Gelatti et al, 2009). Reinert et al (2008), analyzed a culture collection grown between 1995 and 1999 and characterized them by PFGE (electrophoresis in pulsed-field gel). The results showed that the predominant profile (80%) corresponded to the Brazilian Epidemic Clone (BEC).…”

Section: Case Reportsmentioning

confidence: 99%

“…These data showed that the presence of CA-MRSA in Brazil is longstanding and well established and must have passed unnoticed in clinical laboratories. It is necessary to detect and monitor these strains in the community and in hospitals for a better understanding of its epidemiology, as well as to inform public health strategies to control its spread (Reinert et al, 2008).…”

Section: Case Reportsmentioning

confidence: 99%

CA-MRSA: Epidemiology of a Pathogen of a Great Concern

Bonesso¹,

Oliveira²,

Cunha³

2012

Epidemiology Insights

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“…There are no data about the prevalence of SCCmec IV in Brazilian communities, but Reinert et al (22) characterized through pulsed-field gel electrophoresis (PFGE) a culture collection recovered between 1995 and 1999. The results showed that the predominant profile (80%) corresponded to the Brazilian endemic clone (BEC).…”

Section: S Aureus S Epidermidis S Saprophyticus S Haemolyticusmentioning

confidence: 99%

“…Even though the study was performed with hospital samples, these data showed that SCCmec IV has long been present in Brazil and must have passed unnoticed in the laboratory practice. These strains must be detected both in community and in the hospital to better elucidate their epidemiology and control their dissemination (22).…”

Section: S Aureus S Epidermidis S Saprophyticus S Haemolyticusmentioning

confidence: 99%

Untitled

Bonesso

Marques

Cunha

2011

J. Venom. Anim. Toxins incl. Trop. Dis.

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Abstract:Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) are frequently found in nosocomial environments as the main pathogen in several infections. In 1961, reports of nosocomial S. aureus resistant to methicillin, the drug of choice against penicillin-resistant strains, required new alternatives and vancomycin started being used to treat infections caused by methicillin-resistant S. aureus (MRSA). Community-acquired methicillin-resistant S. aureus (CA-MRSA) was first reported in 1990 affecting patients without risk factors for infection with MRSA of hospital origin. MRSA of community origin harbor the genes responsible for the synthesis of Panton-Valentine leukocidin (PVL), a toxin associated with skin and soft tissue infections and that carries the staphylococcal cassette chromosome mec (SCCmec) type IV. CA-MRSA emergence has caused great impact on the worldwide medical community since the presence of this pathogen in patients without risk factors represents a high risk to public health.

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Type IV SCCmec found in decade old Brazilian MRSA isolates

Abstract: Methicillin-resistant

Cited by 16 publications

References 28 publications

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

Molecular characteristic of Staphylococcus aureus isolates in a Chinese teaching hospital

CA-MRSA: Epidemiology of a Pathogen of a Great Concern

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