Utility of the ceftazidime-imipenem antagonism test (CIAT) to detect and confirm the presence of inducible AmpC beta-lactamases among enterobacteriaceae

Cantarelli, Vlademir Vicente; Inamine, Everton; Brodt, Teresa; Secchi, Carina; Cavalcante, Bianca C.; Pereira, Fabiana de Souza

doi:10.1590/s1413-86702007000200014

Cited by 17 publications

(16 citation statements)

References 26 publications

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“…for meropenem and colistin, [2] phenotypic tests for AmpC production (by cefoxitin disc, [3] disc antagonism test, [3] boronic acid inhibition test [4] and ceftazidime-imipenem antagonism test) [5] and test for presence of effl ux pump were performed. [6] Multiplex polymerase chain reaction (PCR) for CTX-M gene 1, 2, 8, 9 and 25 [7] and bla IMP-1 , bla IMP-2 , bla VIM-1 and bla VIM-2 of carbapenemases genes were done.…”

mentioning

confidence: 99%

Multidrug resistant Gram-negative bacilli from neonatal septicaemia at a tertiary care centre in North India: A phenotypic and genotypic study

Srivastava

Agarwal

Srivastava

et al. 2014

Indian Journal of Medical Microbiology

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mentioning

confidence: 99%

Multidrug resistant Gram-negative bacilli from neonatal septicaemia at a tertiary care centre in North India: A phenotypic and genotypic study

Srivastava

Agarwal

Srivastava

et al. 2014

Indian Journal of Medical Microbiology

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“…All the susceptibility test plates were incubated at 30 o C for 18-24 hrs. Antagonism indicated by a visible reduction in the inhibition zone around the ceftazidime disk adjacent to the imipenem or cefoxitin disk was inferred as a positive inducible AmpC β-lactamase production [11,14]. Table 1 shows the rate of recovery of P. aeruginosa isolates from the feacal swab samples of the feacal matter of cow.…”

Section: Confirmatory Test For Ampc β-Lactamase Productionmentioning

confidence: 99%

“…AmpC β-lactamase production in the test isolates was phenotypically confirmed using the ceftazidime-imipenem antagonism test (CIAT) which was performed using ceftazidime (30 µg) disk, cefoxitin (30 µg) disk and imipenem disk (10 µg) as described by Cantarelli et al [14]. Ceftazidime disc and imipenem disk were placed at a distance of 20 mm apart on Mueller-Hinton agar plate previously inoculated with a suspension of the test bacteria (adjusted to 0.5 McFarland turbidity standards) that showed reduced susceptibility to cefoxitin.…”

Section: Confirmatory Test For Ampc β-Lactamase Productionmentioning

confidence: 99%

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Chika¹

2017

JMEN

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The possible contamination of food and/or food-producing animals with multidrug resistant bacteria including AmpC-producing Pseudomonas aeruginosa is considered a potential source for the wide dissemination of AmpC β-lactamase in the community. This portends public health risks for the populace -owing to the multidrug resistant nature of these organisms. There is paucity of data on the prevalence of AmpCproducing bacteria in Abakaliki, Nigeria -which was why this study was carried out. A total of 40 feacal swab samples from cow dung in a local abattoir were bacteriologically examined for the isolation and antimicrobial susceptibility testing of P. aeruginosa isolates using standard microbiological procedures on cetrimide selective agar and Kirby-Bauer disk diffusion method respectively. AmpC β-lactamase was phenotypically confirmed using the ceftazidime-imipenem antagonism test (CIAT). A total of 12 P. aeruginosa isolates were recovered from the samples; and they showed varied levels of resistance to the tested antibiotics especially to cefoxitin, ertapenem, oxacillin, amikacin and cefotaxime. Among the 12 P. aeruginosa isolates, AmpC β-lactamase was phenotypically detected in 3 (25%) isolates by the CIAT method. This study has presumptively shown that AmpC β-lactamase-producing P. aeruginosa isolates occur in abattoir. Thus, molecular characterization of the genes that encode AmpC β-lactamase production in this organism is crucial for a reliable epidemiological investigation into the possible emergence and dissemination of AmpC positive bacteria in the community. monitoring and possibly a ban on the use of antibiotics in the production and/or rearing of food producing animals in order to assuage the emergence and spread of drug resistant bacteria in the community [7,19,21]. Thus, this study presumptively detected the occurrence of AmpC-producing P. aeruginosa isolates from feacal matter of cow in a local abattoir. Keywords: Materials and Methods Sample collection and processingA total of forty (40) swab samples were collected from feacal matter of cow in a local abattoir using sterile swab sticks. After collection, each of the swab sticks was returned into their respective containers, labeled and transported to the Microbiology Laboratory Unit of Ebonyi State University, Abakaliki for bacteriological analysis. Isolation and identification of Pseudomonas aeruginosaCetrimide selective agar (Oxoid, UK) containing 10 ml of glycerol was used for the selective isolation of P. aeruginosa from the test samples. Each of the feacal swab samples was inoculated in 5 ml double strength of freshly prepared nutrient broth (Oxoid, UK) and incubated for 18-24 hours at 30 o C. A loopful of the broth culture was aseptically streaked on cetrimide selective agar plates; and the plates were incubated at 30 o C 18-24 hours. Suspected colonies were inoculated onto freshly prepared cetrimide selective agar for the isolation of pure cultures of P. aeruginosa. P. aeruginosa produces greenish/bluish pigmentation on cetrimide selec...

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“…These isolates were carrying CIT gene. Cantarelli et al (2007) found that CIAT detected chromosomal AmpC more perfectly than PMABLs as 100% of isolates were induced by imipenam, two (6%) were carrying plasmid (blaDHA) and 32 (94%) isolates were chromosomal. However, in our study there were 16 isolates that were carrying CIT gene, but were negative by the test; These islolates were resistance to ceftazidime and were having other Amp C genes which may inhibit or mask the effect of the inducer (imipenem).…”

mentioning

confidence: 97%

“…Also, Cantarelli et al, (2007) stated that, CIAT test can not be used to test strains showing no inhibition zone for ceftazidime or for those strains bearing plasmidmediated AmpC enzymes that are not typically inducible.…”

mentioning

confidence: 99%

Evaluation of Phenotypic Methods for Detection of Plasmid-Mediated AmpC β-Lactamases (PMABLs) among Klebsiella pneumoniae

Othman¹,

ElHamid²

2016

Int.J.Curr.Microbiol.App.Sci

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Utility of the ceftazidime-imipenem antagonism test (CIAT) to detect and confirm the presence of inducible AmpC beta-lactamases among enterobacteriaceae

Cited by 17 publications

References 26 publications

Multidrug resistant Gram-negative bacilli from neonatal septicaemia at a tertiary care centre in North India: A phenotypic and genotypic study

Multidrug resistant Gram-negative bacilli from neonatal septicaemia at a tertiary care centre in North India: A phenotypic and genotypic study

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Evaluation of Phenotypic Methods for Detection of Plasmid-Mediated AmpC β-Lactamases (PMABLs) among Klebsiella pneumoniae

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