Enrichment methodology to increase the positivity of cultures from body fluids

Daur, Alessandra Valle; Jr., Francisco Klimak; Cogo, Laura Lúcia; Botão, Gislene Diógenes; Monteiro, Cristina; Costa, Libera Maria Dalla

doi:10.1590/s1413-86702006000600002

Cited by 14 publications

(17 citation statements)

References 7 publications

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“…The overall 14.1% (n=54/384) prevalence of bacteria from body fluids was relatively lower than the finding from Brazil study [17]. This might be due to over diagnosing, prior exposure to antibiotics and emergence of non-infectious conditions like malignancy.…”

Section: Discussioncontrasting

confidence: 55%

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Teklehymanot¹,

Legese²,

Desta³

2017

Biol Med

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Background: Infections caused by multidrug resistant (MDR) bacteria remain a public health threat for patients and health care workers. There are scarcity of data on bacterial profiles and their drug susceptibility patterns from body fluids in Ethiopia. Hence, this study aimed at assessing bacterial profiles and their antimicrobial susceptibility patterns (AST) from body fluids at Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia.

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Section: Discussioncontrasting

confidence: 55%

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Teklehymanot¹,

Legese²,

Desta³

2017

Biol Med

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“…Thereafter, the organism was repeatedly isolated by blood culture bottles. The method using the blood culture bottles produced a significant and consistent increase in the isolation rate for body fluid cultures compared to conventional method 10 . Therefore, we cannot exclude that D. acidovorans may be the causative agent for the empyema.…”

Section: Discussionmentioning

confidence: 88%

Delftia acidovorans Isolated from the Drainage in an Immunocompetent Patient with Empyema

et al. 2009

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Delftia acidovorans is a gram-negative motile rod found ubiquitously in soil and in water. Confirmed isolation from clinical infections is rare, and has been documented mostly in immunocompromised patients or those with indwelling catheters. A 53-year-old man was referred for the evaluation of a huge mass-like lesion found incidentally by chest X-ray. The lesion occupied more than half of the right lung and was diagnosed as a large loculated pleural effusion by CT scan. Bloody pus was drained through a percutaneous catheter, and D. acidovorans, identified by the Vitek GN card and confirmed by amplification of 16S ribosomal RNA and sequencing analysis, was isolated repeatedly from the drained pus. The patient was treated with imipenem/cilastatin to which the organism was sensitive. This is a rare report of chronic empyema associated with D. acidovorans in the respiratory system of an immunocompetent patient.

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“…Daur et al [1] reported that Pseudomonas aeruginosa and Escherichia coli were the most frequently isolated pathogens in their study. Similar finding were also reported by Lakshmi et al [5] from Hyderabad who found that the enteric Gram negative bacilli were the predominant pathogens.…”

Section: Discussionmentioning

confidence: 92%

“…The different enrichment culture systems include trypticase-soy broth, [1] Soyabean-Casein digest(SCD) broth, Brain-Heart infusion broth [2] and automated systems like BACTEC [3] and BacT Alert. [4,5] The present study was planned to identify common organisms isolated from various body fluids along with their antimicrobial susceptibility pattern.…”

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confidence: 99%

Bacteriology of Body Fluids With an Evaluation of Enrichment Technique to Increase Culture Positivity

Deb¹,

Mudshingkar²,

Dohe³

et al. 2014

jemds

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BACKGROUND: Body fluids like ascitic fluids, pleural fluids, cerebrospinal fluids (CSF) etc. are sent for culture in a clinical microbiology laboratory to achieve etiological diagnosis. However the yield of such cultures is usually very low. So, ongoing monitoring of prevalent pathogenic organisms and their sensitivities help the clinicians institute therapy in absence of a culture report. AIMS: The study was done to identify the common pathogens isolated from body fluids along with their antimicrobial susceptibility pattern and also to evaluate the impact of enrichment on their culture positivity. SETTING AND DESIGN: A 3-month prospective analytical study was done in a tertiary care hospital. MATERIALS AND METHODS: A total of 333 Body fluids were processed; 103 of them were ascitic fluids, 71 pleural fluids, 139 CSF and 20 other fluids. They were processed by plating the direct sample and after enrichment. Enrichment was done by two methods: in SoyabeanCaesin digest broth (274 samples) and by BACTEC (59 samples).Isolates were identified by routine procedures & their antimicrobial susceptibility determined as per CLSI guidelines. The results were analyzed using Microsoft Excel® software using p<0.05 as the cut-off for significance. RESULTS: Gram negative isolate were obtained from 21.3% of the samples. The common isolates were Pseudomonas (20.7%), Acinetobacter (11.6%), Citrobacter (10.7%) and E. coli (10.7%). The antibiotics most effective against Gram negative pathogens were Gentamicin (47.5%), PipercillinTazobactam (51.6%), Amikacin (56.7%) and Cefoperazone-Sulbactam(65.3%). Gram positive isolates, obtained from 9% of the samples, mostly consisted of MSSA, Enterococcus and CONS, for which Ciprofloxacin (48%) followed by Cotrimoxazole (40%) and Erythromycin (28.6%) showed reasonable efficacy. The Culture positivity with direct plating, Soyabean-Caesin broth enrichment and BACTEC was 14.41%, 29.19% and 42.37% respectively. Increase in positivity by Soyabean-Casein broth was maximum for pleural fluids (12%) followed by ascitic fluids (11.6%) and CSF (11.52%).Using automated system the corresponding increases were 20.7%for ascitic fluids and 5.4%for pleural fluids. The mean time for identification using direct plating, enrichment method and BACTEC were 48 hours, 72 hours and 40 hours respectively. CONCLUSION: Gram negative isolates are commonly isolated pathogens from body fluids in our setup. Enrichment of body fluids improved yield of pathogens. In resource-poor settings simple enrichment in blood culture bottles can increase culture positivity of these precious samples.

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Enrichment methodology to increase the positivity of cultures from body fluids

Cited by 14 publications

References 7 publications

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Bacterial Profile and their Antimicrobial Resistance Patterns from Body Fluids at Tikur Anbesa Specialized Hopital, Addis Ababa, Ethiopia

Delftia acidovorans Isolated from the Drainage in an Immunocompetent Patient with Empyema

Bacteriology of Body Fluids With an Evaluation of Enrichment Technique to Increase Culture Positivity

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