Leishmania mexicana infection of the eyelid in a traveler to Belize

Vinetz, Joseph M.; Soong, Lynn

doi:10.1590/s1413-86702006000400016

Cited by 8 publications

(8 citation statements)

References 22 publications

(5 reference statements)

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“…Promastigotes were cultured at 23°C in Schneider's Drosophila medium (Invitrogen, Grand Island, NY), pH 7.0, supplemented with 20% fetal bovine serum (FBS; HyClone, Logan, UT), 2 mM L-glutamine, and 50 g/ml gentamicin. L. mexicana promastigotes, which were originally isolated from a patient (53), were cultured at 26°C in M199 medium. Unless specified, the stationary-stage promastigotes were used in all of the experiments.…”

Section: Methodsmentioning

confidence: 99%

Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

Wang

Chen

Luo³

et al. 2011

Infect Immun

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Recent studies have shown that histone proteins can act as antimicrobial peptides in host defense against extracellular bacteria, fungi, and Leishmania promastigotes. In this study, we used human recombinant histone proteins to further study their leishmaniacidal effects and the underlying mechanisms. We found that the histones H2A and H2B (but not H1(0)) could directly and efficiently kill promastigotes of Leishmania amazonensis, L. major, L. braziliensis, and L. mexicana in a treatment dose-dependent manner. Scanning electron microscopy revealed surface disruption of histone-treated promastigotes. More importantly, the preexposure of promastigotes to histone proteins markedly decreased the infectivity of promastigotes to murine macrophages (Mφs) in vitro. However, axenic and lesion-derived amastigotes of L. amazonensis and L. mexicana were relatively resistant to histone treatment, which correlated with the low levels of intracellular H2A in treated amastigotes. To understand the mechanisms underlying these differential responses, we investigated the role of promastigote surface molecules in histone-mediated killing. Compared with the corresponding controls, transgenic L. amazonensis promastigotes expressing lower levels of surface gp63 proteins were more susceptible to histone H2A, while L. major and L. mexicana promastigotes with targeted deletion of the lipophosphoglycan 2 (lpg2) gene (but not the lpg1 gene) were more resistant to histone H2A. We discuss the influence of promastigote major surface molecules in the leishmaniacidal effect of histone proteins. This study provides new information on host innate immunity to different developmental stages of Leishmania parasites.

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Section: Methodsmentioning

confidence: 99%

Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

Wang

Chen

Luo³

et al. 2011

Infect Immun

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“…Lesion-derived amastigotes remained in the amastigote form under these culture conditions. L. mexicana (TG) axenic amastigotes were obtained from a patient in 2002 (37) and were maintained as the amastigote form in Schneider's Drosophila media (pH 5.0) at 33°C. pM cultures.…”

Section: Methodsmentioning

confidence: 99%

l-Arginine and Cationic Amino Acid Transporter 2B Regulate Growth and Survival ofLeishmania amazonensisAmastigotes in Macrophages

Wanasen¹,

MacLeod

Ellies

et al. 2007

Infect Immun

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“…Cutaneous leishmaniasis is the most common clinical outcome . In Mexico and many regions of Central America, Leishmania mexicana is the main species causing LCL and DCL in humans and animals …”

Section: Introductionmentioning

confidence: 99%

Th17 cells and neutrophils: Close collaborators in chronic Leishmania mexicana infections leading to disease severity

Pedraza-Zamora

Delgado-Domínguez

Zamora‐Chimal

et al. 2017

Parasite Immunology

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Cutaneous leishmaniasis caused by Leishmania mexicana is associated with an important inflammatory response. We here analysed the kinetics of Th17 cells and neutrophils in ear lobe lesions caused by Leishmania mexicana throughout 90 days of disease progression in susceptible BALB/c and semi-resistant C57BL/6 mice infected with 1 × 10 Leishmania mexicana promastigotes. Cells in the lesions were extracted and quantified by flow cytometry, whereas their distribution in the tissues in relation to the parasites was analysed by immunohistochemistry. Our results show that in BALB/c mice, both Th17 cells and neutrophils increase concomitantly and to significantly higher levels on day 90 post-infection, as compared to C57BL/6 mice. Our results provide novel evidence on the cells causing chronic inflammation throughout Leishmania mexicana infections, resulting as a consequence of neutrophil recruitment together with Th17 cell differentiation and recruitment, both of which remain in the infection site throughout the late phase of the infection. We conclude that the more enhanced levels of Th17 cells and neutrophils during chronic inflammatory lesions in BALB/c mice participate in their enhanced susceptibility towards a progressive disease evolution, whereas the more controlled response of these cells in C57BL/6 mice possibly relates to the more resistant profile of this mouse strain.

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Leishmania mexicana infection of the eyelid in a traveler to Belize

Cited by 8 publications

References 22 publications

Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

l-Arginine and Cationic Amino Acid Transporter 2B Regulate Growth and Survival ofLeishmania amazonensisAmastigotes in Macrophages

Th17 cells and neutrophils: Close collaborators in chronic Leishmania mexicana infections leading to disease severity

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