Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the Municipality of Rio de Janeiro, Brazil

Madeira, Maria de Fátima; Schubach, Armando de Oliveira; Schubach, Tânia Maria Pacheco; Leal, Cristianni Antunes; Marzochi, Mauro Célio de Almeida

doi:10.1590/s1413-86702004000600008

Cited by 31 publications

(25 citation statements)

References 13 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The successful infection of laboratory-bred L. longipalpis fed on two of three randomly selected dogs from our asymptomatic group 3 , however, tends to support the conclusion that dogs with inapparent CVL are of importance in the maintenance of AVL foci 21 . A similar conclusion was reached regarding the transmission of the closely related Leishmania (L.) infantum infantum in the Mediterranean region of the Old World, where the vector Phlebotomus perniciosus was successfully infected when fed on asymptomatic dogs 23 .…”

mentioning

confidence: 51%

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Lima

Carneiro

Campos

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Canine visceral leishmaniasis (CVL) is recognizable by characteristic signs of disease and is highly lethal. The infection, however, may be quite inapparent in some seropositive dogs, and this has raised the polemic question as to whether or not such animals can be a source of infection for Lutzomyia longipalpis, the vector of American visceral leishmaniasis (AVL). In this study we have examined 51 dogs with acute CVL from an AVL area in Pará State, northern Brazil, and compared the parasite density, amastigotes of Leishmania (L.) infantum chagasi, in the skin, lymph node and viscera of symptomatic with that of nine asymptomatic but seropositive dogs (IFAT-IgG). Post-mortem biopsy fragments of these tissues were processed by immunohistochemistry, using a polyclonal antibody against Leishmania sp. The X² and Mann Whitney tests were used to evaluate the means of infected macrophage density (p < 0.05). There was no difference (p > 0.05) in the skin (10.7/mm² x 15.5/mm²) and lymph node (6.3/mm² x 8.3/mm²), between asymptomatic and symptomatic dogs, respectively. It was higher (p < 0.05), however, in the viscera of symptomatic (5.3/mm²) than it was in asymptomatic (1.4/mm²) dogs. These results strongly suggest that asymptomatic or symptomatic L. (L.) i. chagasi-infected dogs can serve as a source of infection, principally considering the highest (p < 0.05) parasite density from skin (10.7/mm² x 15.5/mm²), the place where the vetor L. longipalpis takes its blood meal, compared with those from lymph node (6.3/mm² x 8.3/mm²) and viscera (1.4/mm²x 5.3/mm²).

show abstract

mentioning

confidence: 51%

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Lima

Carneiro

Campos

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…In two studies conducted in the Municipality of Rio de Janeiro, MADEIRA et al 16,17 isolated Leishmania from 18/20 and from 31/39 animals, respectively, using skin fragments obtained from dogs euthanized because of a suspicion of CVL for parasitological confirmation. In another study, the same authors reported a rate of isolation of 78.7% when skin samples from 394 dogs were evaluated 15 .…”

Section: Discussionmentioning

confidence: 99%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

show abstract

“…A presença de 50 a 70% de cães assintomáticos em áreas endêmicas, potencialmente infectantes para o vetor 12 , denota a importância dos inquéritos sorológicos caninos para que se antecipem as ações profiláticas, já que os casos caninos precedem a doença no homem 18 . Nesta investigação, a porcentagem obtida de cães assintomáticos foi de 56,2%, não divergindo do cenário encontrado no país.…”

Section: Discussionunclassified

Inquérito soroepidemiológico de leishmaniose canina em áreas endêmicas de Cuiabá, Estado de Mato Grosso

Almeida

Faria

Pimentel

et al. 2009

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

As leishmanioses são zoonoses em expansão no Brasil, tendo o cão importância na transmissão e dispersão da doença, principalmente em áreas de leishmaniose visceral. O objetivo desta pesquisa foi avaliar a soroprevalência de leishmaniose em cães domiciliados na zona urbana de Cuiabá. Para a pesquisa foram selecionados quatro bairros de Cuiabá, sendo um em cada regional administrativa. A amostragem canina foi definida estatisticamente, considerando-se a prevalência de 8,4%. Dos 468 cães analisados, 16 foram reagentes na imunofluorescência indireta, obtendo-se uma prevalência geral de 3,4%. Não foi observada predisposição racial, sexual e etária para a ocorrência da leishmaniose canina. Os principais fatores de risco identificados na ocorrência da infecção canina na Cidade de Cuiabá, foram a localização dos cães no peridomicílio, bem como a proximidade das residências de matas, evidenciando mudanças na ocorrência da doença no ambiente urbano.

show abstract

Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the Municipality of Rio de Janeiro, Brazil

Cited by 31 publications

References 13 publications

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Inquérito soroepidemiológico de leishmaniose canina em áreas endêmicas de Cuiabá, Estado de Mato Grosso

Contact Info

Product

Resources

About