Laser 830nm na viabilidade do retalho cutâneo de ratos submetidos à nicotina

Abstract

Objective: To investigate the possible effects of laser diode 830nm counter to the action of nicotine on the viability of the skin flap in rats. Methods: 24 Wistar-albino rats were used, divided into 3 groups of 8: Group 1 -subject to the surgical technique to obtain the random skin flap in cranial base, subcutaneous injection of nicotine of 2mg/Kg/day a week before and one week after surgery and simulation of laser. Group 2 -similar to group 1, the random skin flap in the cranial base were subjected to laser irradiation immediately after surgery and on the subsequent 4 days. After euthanasia, areas of necrosis and viable tissue were examined by a manual method (weighing the Role of Feedback) and by the semi-automated method of analysis (Mini-Mop ® ). Results: The results were statistically analyzed by ANOVA. The values of the percentage of necrotic area by the method of analysis showed a decreased area of necrosis in group 2 where the laser was applied. Conclusion: The 830nm laser was effective in improving the viability of skin flaps in rats subjected to the action of nicotine. Level of evidence II: Therapeutic Studies -Investigating the results of treatment.

Viabilidade do retalho cutâneo randômico dorsal em camundongos submetidos à fotobiomodulação e ultrassom terapêutico

“…Furthermore, PBM helps in angiogenesis, reducing the percentage of tissue necrosis and consequently increasing the viable area [6][7][8][20][21][22][23][24] . The parameters used in G5 coincide with the literature, which indicates the wavelength of 830 nm is more promising in neoangiogenesis and tissue healing 23 when compared to 630 nm.…”

“…Once the local blood flow is sufficient, especially in the distal part of the flap, necrosis is no longer a problem 6 . Thus, studies have been conducted aiming to increase the local blood supply with non-invasive resources, such as Photobiomodulation (PBM) 7,8 . The effects of PBM in the biological system depend on the amount of energy absorbed and the rate of energy deposition 9 so that the wavelength used determines the dispersion and absorption of light 9,10 .…”

“…The choice of TUS parameters in the present study was based on previous literature, which showed positive effects on wound healing in rats 14,16 . The PBM parameters were based on previous studies [7][8][9]12 , which used wavelengths of 660 and 830 nanometers.…”

Efeito da estimulação elétrica de alta voltagem na integração de enxertos de pele total em ratos submetidos à ação da nicotina

“…A viabilidade de retalhos experimentais afetados pela exposição a nicotina tem sido estudada, demonstrando a influência deletéria da substância (Kaufman et al, 1984;Ely et al, 2009;Selçuk et al, 2012;Das Neves et al, 2011;Neves et al, 2011;Shah et al, 2012;Neves et al, 2016.). No presente estudo foi observado que o grupo que foi submetido a nicotina e não recebeu nenhuma estimulação, apresentou a maior porcentagem de contração do enxerto, fato que pode indicar a provável influência negativa da nicotina na sua integração, por promover isquemia, e por consequência diminuição do fluxo sanguíneo no local (Silverstein, 1992).…”

“… Grupo 4 (Nicotina)animais submetidos à injeção de nicotina injetada na tela subcutânea em uma dose de 2 mg/Kg, 2x ao dia, por um período de 28 dias até o procedimento cirúrgico, e posteriormente, submetidos ao procedimento cirúrgico de enxerto de pele (Neves et al, 2011).…”