Modelo de suspensão pela cauda e seu efeito em algumas propriedades mecânicas do osso do rato

Silva, Adriana Valadares da; Volpon, José Batista

doi:10.1590/s1413-78522004000100004

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Furthermore, we observed that jump training prevented loss of body mass. These findings are in agreement with other studies ( 18 , 23 , 29 , 30 ).…”

Section: Discussionsupporting

confidence: 94%

High-impact exercise in rats prior to and during suspension can prevent bone loss

Yanagihara

Paiva

Gasparini

et al. 2016

Braz J Med Biol Res

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High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double energy X-ray absorptiometry and the same bones were evaluated by mechanical tests. Bone microarchitecture was evaluated by scanning electron microscopy. One-way ANOVA was used to compare groups. Significance was determined as P<0.05. Regarding bone mineral density, mechanical properties and bone microarchitecture, the beneficial effects were greater in the bones of animals subjected to pre-suspension training and subsequently to training during suspension, compared with the bones of animals subjected to pre-suspension training or to training during suspension. Our results indicate that a period of high impact exercise prior to tail suspension in rats can prevent the installation of osteopenia if there is also training during the tail suspension.

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“…Furthermore, we observed that jump training prevented loss of body mass. These findings are in agreement with other studies ( 18 , 23 , 29 , 30 ).…”

Section: Discussionsupporting

confidence: 94%

High-impact exercise in rats prior to and during suspension can prevent bone loss

Yanagihara

Paiva

Gasparini

et al. 2016

Braz J Med Biol Res

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“…However, this procedure may lead to hemostatic and morphofunctional disorders in bone tissue, with a consequent decrease of their mechanical properties. (2,15) In this study, the used immobilization generated a reduction in the number of osteocytes and a reduction in the diameter of the medullary canal (µm) in all immobilized groups when compared to CG. Nonetheless, the cortical thickness was significantly reduced only in the ICG and IMG when compared to the CG.…”

Section: Discussionmentioning

confidence: 82%

“…(15) This result seems to be associated with the decrease in bone metabolism, which causes a decrease in the cells number of the maintenance of this tissue. (15)(16)(17)(18) According to studies by Yeh et al, (16) the immobilization protocol allows the reabsorption and depression of the bone formation, results in decreased bone mass relative to its volume and simultaneously changes the material and geometric properties of the bone. It is estimated that about 30% of the bone loss induced by immobilization experiment in rats is caused by increase of bone resorption and about 70% by decreased bone formation.…”

Section: Discussionmentioning

confidence: 99%

Joint immobilization: effects on bone tissue of obese and malnourished animals.

et al. 2020

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Introduction: The immobilization induces an unbalanced bone metabolism, followed by rapid bone loss and consequent loss of the mechanical function of the bone. In general, the obesity and protein malnutrition conditions affect a large number of people worldwide, and both morbidities have specific characteristics that may cause deleterious effects on bone tissues of patients by different mechanisms. Objective: the present study aimed to verify experimentally if the joint immobilization protocol causes bone tissue atrophy in obese and undernourished animals. Method: 20 adult male mice (C57/BL6) were used, divided into four groups (N=5): Control Group (CG), Immobilized Control Group (ICG), Immobilized Obese Group (IOG) and Immobilized Malnourished Group (IMG). The histomorphometric analysis of the tibia quantified the number of osteocytes, cortical thickness and diameter of the medullary canal. Results: The study involving the tibia of the animals showed statistical differences in variables analysis. All immobilized groups showed lower amount of osteocytes in the evaluated tissue and increase in diameter of the spinal canal when compared to the CG. The cortical thickness was reduced in ICG and IMG groups when compared to the CG. Conclusion: The used joint immobilization protocol caused bone atrophy in the studied animals. The association between obesity, malnutrition and joint immobilization conditions cause increase in bone tissue atrophy.

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“…The animals included on groups S, S-R and S-T were suspended by tail for 28 days, and all procedures for hanging the animals by the tail were based on the study by Silva and Volpon (12) , who used a suspension model developed from a description by Kasper et al (13) . For preparing animals' tails for suspension, the rats were anesthetized by intramuscular injection of a combination of 30mg/kg and 3mg/kg ketamine and xylazine, respectively.…”

Section: Animal Suspensionmentioning

confidence: 99%

Comportamento mecânico do terço proximal de fêmures de ratos após período de suspensão pela cauda e exercitação

Shimano

Volpon

2007

Acta ortop. bras.

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A remodelação óssea pode ser estimulada por forças mecânicas presentes nas atividades físicas normais. Neste trabalho foi analisado o comportamento mecânico do terço proximal de fêmur de ratas submetidas à suspensão pela cauda e posterior treinamento em esteira. Sessenta e seis ratas da raça Wistar foram usadas. Primeiramente os animais foram criados por noventa dias e divididos em cinco grupos (dois controles e três experimentais). Os animais do grupo Controle I foram sacrificados com 118 dias de idade. No grupo S (suspenso) os animais foram suspensos pela cauda por 28 dias e sacrificados. No grupo Controle II os animais foram sacrificados com 139 dias de idade. No grupo S-L (suspenso-liberado) as ratas foram liberadas 21 dias após a suspensão. No grupo S-T (suspenso-treinado) após o período de suspensão os animais passaram por treinamento em esteira durante 21 dias. Para análise do comportamento mecânico do osso foi aplicada uma força vertical na cabeça femoral até a ruptura. A fratura foi analisada por raios-X. A suspensão causou um decréscimo da força máxima e, o treinamento e a liberação após a suspensão causaram a recuperação das propriedades mecânicas. Mas, o padrão de fratura não apresentou diferença entre os grupos experimentais.

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Modelo de suspensão pela cauda e seu efeito em algumas propriedades mecânicas do osso do rato

Abstract: silience). 53 adult female rats were used and distributed in four groups according to the length of time in suspension (control, 7, 14 and 21 days). The model of suspension was efficient with good animals adaptation and it caused a significant weakness of bones mainly in the 21-day period.

Cited by 8 publications

References 11 publications

High-impact exercise in rats prior to and during suspension can prevent bone loss

High-impact exercise in rats prior to and during suspension can prevent bone loss

Joint immobilization: effects on bone tissue of obese and malnourished animals.

Comportamento mecânico do terço proximal de fêmures de ratos após período de suspensão pela cauda e exercitação

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