Alterações morfofisiolócias de plantas de abacaxizeiro influenciadas por diferentes substratos durante o processo de aclimatização

Braga, Francyane Tavares; Pasqual, Moacir; Castro, Evaristo Mauro de; Rafael, Gabriel Coimbra; Favero, Ana Carolina; Valente, Thaís Cainã Teixeira

doi:10.1590/s1413-70542011000500001

Cited by 4 publications

(4 citation statements)

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“…Braga et al (2011) relatam que plantas que se desenvolveram heterotroicamente sob condições de cultivo in vitro, em alta umidade, passam por um período de adaptação, quando submetidas a condições autotróicas, com baixa umidade durante a aclimatização, em razão da grande diferença entre os dois ambientes. Nesse contexto, os resultados indicam que P. hispidinervum desenvolveu uma estratégia de armazenamento de água na hipoderme como uma adaptação para suportar as condições ex vitro.…”

Section: Para Os Estudos Anatômicos Foram Utilizados O Terço Médio Dunclassified

“…O cultivo in vitro pode causar alterações anatômicas, morfológicas e isiológicas nas plantas, prejudicando a aclimatização das plantas micropropagadas (ALBARELLO et al, 2001;CALVETE et al, 2002). Os brotos desenvolvidos in vitro estão expostos a um microambiente controlado, organizado para gerar estresse mínimo, mas com ótimas condições para a multiplicação (hAzARiKA, 2006).…”

Section: Introductionunclassified

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Anatomia comparada de folhas de pimenta longa (Piper hispidinervum C. DC.) e pimenta de macaco (Piper aduncum L.) cultivadas in vitro, ex vitro e in vivo

et al. 2014

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ResumoPiper hispidinervum e Piper aduncum apresentam compostos metabólitos secundários como safrol e dilapiol de interesse comercial em seus óleos essenciais. O trabalho teve como objetivo comparar aspectos anatômicos, relacionados a respostas isiológicas das folhas de P. hispidinervum e P. aduncum propagadas in vitro, in vivo e durante a aclimatização. Secções paradérmicas e transversais da lâmina foliar do cultivo in vitro, ex vitro e in vivo, feitas à mão-livre foram realizadas para a medição das estruturas anatômicas em microscópio de luz. A espessura da epiderme e hipoderme de P. hispidinervum e P. aduncum sofrem alterações na transição de cultivo in vitro para o ex vitro. Os tecidos do mesoilo e a abertura do poro estomático de ambas as espécies são inluenciados pelo ambiente in vitro. Diferentes ambientes de cultivo promovem a plasticidade das estruturas celulares da lâmina foliar e fundamentam o sucesso da micropropagação de ambas as espécies. Palavras-chave: Amazônia Sul-Ocidental; Micropropagação; Piperaceae; Variação estrutural AbstractComparative leaf anatomy of long pepper (Piper hispidinervum C. DC.) and spiked pepper (Piper aduncum L.) cultured in vitro, ex vitro and in vivo. Piper hispidinervum and Piper aduncum contain the secondary metabolites safrole and dilapiol, and there is commercial interest in their essential oils. The study aimed to compare anatomical aspects related to physiological responses of leaves from P. hispidinervum and P. aduncum propagated in vitro, in vivo and during acclimatization. Paradermal sections and cross-sections of leaves from in vitro, ex vitro and in vivo culture, were obtained for the measurement of anatomical structures using a light microscope. The thickness of the epidermis and hypodermis of P. hispidinervum and P. aduncum underwent changes in the transition from in vitro to ex vitro. Mesophyll tissues and stomatal pore opening of both species were inluenced by the environment in vitro. Different cultivation environments promote the plasticity of the cellular structures of the leaf blade and underlie the success of micropropagation of both species.

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Section: Para Os Estudos Anatômicos Foram Utilizados O Terço Médio Dunclassified

Section: Introductionunclassified

Anatomia comparada de folhas de pimenta longa (Piper hispidinervum C. DC.) e pimenta de macaco (Piper aduncum L.) cultivadas in vitro, ex vitro e in vivo

et al. 2014

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“…Among the main causes of functional disorders in plants ex vitro are the imperfect functioning of the stomatal apparatus, poor development of the cuticle and non-functioning root system [13,19,20]. In this regard, some authors reasonably recommend the use of structural changes monitoring in the vegetative organs of investigated plants adapted ex vitro for the development and optimization of the clonal micropropagation protocol [14,[21][22][23][24][25][26], as well as to study their functional abilities [27,28].…”

Section: Introductionmentioning

confidence: 99%

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

et al. 2021

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Peach [Prunus persica (L.) Batsch] is among the most demanded fruit crops in the world. Biotechnological methods help to originate new hybrid forms in order to increase the cultivar diversity and create new valuable genotypes. Cross combinations between the cultivars Clyde Wilson, Jerseyglo, Loadel, Summerglo and the promising cultivar ‘Nikitskiy Podarok’ have been done. The embryos of these hybrids germinated and formed plantlets after stratification at 4 °C for 45–60 days. The best regeneration rates in the hybrids ‘Loadel’ × ‘Nikitskiy Podarok’ and ‘Summerglo’ × ‘Nikitskiy Podarok’ (96.30% and 92.59%, respectively) were noted on hormone-free Monnier culture medium supplemented with 400.0 mg L−1 casein hydrolyzate. When the newly formed plantlets had necrosis of the shoot apex or immature roots, nodal shoot segments were used. At the same time, a high regeneration capacity was noted in the hybrids ‘Summerglo’ × ‘Nikitskiy Podarok’ and ‘Loadel’ × ‘Nikitskiy Podarok’ on B5 culture medium with 0.75 mg L−1 6–benzyl–aminopurine (BAP) + 0.1 mg L−1 indole–3–butyric acid (IBA). After the second subculture, the number of new adventitious shoots was 5.18 ± 0.18 and 4.95 ± 0.18 shoots per explant, respectively. The plants obtained from the hybrid embryos in a soil mixture soil: peat: sand (3:1:1) were adapted. The main morphological and anatomical features of the leaf blades in newly originated peach hybrids have been studied: the thickness of their tissues and the distribution of stomatal apparatus, as well as the physiological parameters of the photosystem II activity in regenerants cultured in vitro and during their in vivo acclimatization. The high capacity to post aseptic adaptation in the obtained hybrids has been shown.

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“…As a result, in vitro cultivated pla nts h ave morphol ogica l and physi ologi cal abnormaliti es (BARBOZA et al, 2006). Those modifications make them very sensitive to the abrupt environmental changes experienced when they are removed from culture containers and placed under ex vitro conditions (KELLER; LUTTGE, 2005;BRAGA et al, 2011). As a result, a gradual acclimatization period is normally required after ex vitro transplantation to reduce stress as in vitro plants progressively acclimatize to the new growing condition (YANES; GONZÁLEZ; RODRÍGUEZ, 2000).…”

Section: Introductionmentioning

confidence: 99%

Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization

et al. 2012

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Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L.) Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate) were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D') leaf (cm) or plant length (cm). However, after 45 days plant fresh weight (g), length and width of the 'D' leaf (cm) and root number all increased significantly, while transpiration (mmol H2O m-2 s-1) declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.). Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.

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Alterações morfofisiolócias de plantas de abacaxizeiro influenciadas por diferentes substratos durante o processo de aclimatização

Cited by 4 publications

References 3 publications

Anatomia comparada de folhas de pimenta longa (Piper hispidinervum C. DC.) e pimenta de macaco (Piper aduncum L.) cultivadas in vitro, ex vitro e in vivo

Anatomia comparada de folhas de pimenta longa (Piper hispidinervum C. DC.) e pimenta de macaco (Piper aduncum L.) cultivadas in vitro, ex vitro e in vivo

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization

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