Teste de alelismo entre os mutantes de amadurecimento alcobaça e non-ripening em tomateiro

Abstract

RESUMODesde o início da década de 1980, são relatadas na literatura divergências quanto às relações de alelismo ou não entre os mutantes de amadurecimento de frutos de tomateiro denominados alc (= alcobaça) e nor (=non-ripening). Para dirimir tais dúvidas, foi realizado um teste de alelismo entre os genes considerados. Foram avaliadas 364 plantas F 2 provenientes do cruzamento entre as linhagens de tomateiro TOM-559 (alc/alc) e TOM-613 (nor/nor), além de vinte plantas de cada uma das linhagens TOM-559 (alc/ alc), ABSTRACTSince the early 1980's there are conflicting reports on the possible allelic relations between the tomato ripening mutants alc (=alcobaça) and nor (=non-ripening). In order to end these controversies, a test of allelism between the genes alc and nor was performed. A total of 364 plants of the F 2 population between the tomato lines TOM-559 (alc/alc) and TOM-613 (nor/nor) were screened, along with 20 plants each of lines TOM-559 (alc/alc) and TOM-613 (nor/nor), of hybrids F 1 . TOM-559 and TOM-613 are near-isogenic lines to Floradade, and differ from the latter only due to the presence of genes alc and nor, respectively. Floradade fruit harvested at the breaker stage show normal red color (normal phenotype) when fully ripe, whereas fruit of either TOM-559 or TOM-613 remain yellow or yellowish-orange (mutante phenotype). Four fruits per plant were harvested at the breaker stage and subsequently evaluated for their mature fruit color phenotype (normal or mutant). The results of the test of allelism indicate that the most likely hypothesis is that alc and nor are allelic to each other. Therefore, alc was considered to be a third allele at the nor locus, and the symbol nor A was substituted for alc. Há na literatura descrição de vários alelos mutantes que interferem no processo natural de amadurecimento dos frutos do tomateiro, retardando o amadurecimento e prolongando a conservação em pós-colheita, dentre os quais destacam-se os alelos rin (ripening inhibitor), nor (non ripening), alc (alcobaça). Vários trabalhos foram realizados, utilizando esses alelos em heterozigose ou duplas combinações heterozigotas, com a finalidade de se aumentar o tempo de conservação dos frutos em pós-colheita (Mutschler et al., 1992;Freitas et al., 1998;Andrade Junior et al., 2005;Santos Junior et al., 2005; Faria et al., 2006).

Effect of pre-harvest calcium silicate on post-harvest quality of tomatoes

“…'Ivety' showed a higher respiration rate of 6,82 mg CO2 kg -1 h -1 (Table 1). This difference between hybrids can be explained by the fact that they present a completely different genetic characteristic, where the hybrid Natalia presents the rin gene, which is an improved hybrid and present in its natural process the delayed maturation, which prolongs the post-harvest conservation (Benites et al, 2010).…”

Mediterranean Long Shelf-Life Landraces: An Untapped Genetic Resource for Tomato Improvement

“…Several studies support that alc and nor mutations are allelic (Tigchelaar et al, 1976;Lobo et al, 1984;Benites et al, 2010;Casals et al, 2012;Kumar et al, 2018). Recently, studies of NACfamily genes described diverse nor alleles and nor-like genes involved in the tomato ripening process (Wang et al, 2018).…”

The Effect of Replacing Wildrye Hay with Mulberry Leaves on the Growth Performance, Blood Metabolites, and Carcass Characteristics of Sheep

“…The mulberry tree is a multi-purpose species that thrives in a variety of climates from the temperate zone to the tropics. It produces fruit, and its leaves are mainly used for sericulture, traditional herbs, and animal feeds [2]. The cultivated area of mulberry is estimated to be more than one million hectares, and the biological yield of fresh mulberry leaves is about 25-30 tons/hectare/year in China [3].…”