Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

Abstract

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

“…A interação citocinina e auxina não favoreceu a formação de brotos em T. guianensis; provavelmente, a presença de ANA, nas dosagens testadas, pode ter provocado uma diminuição nos níveis de substâncias endógenas naturais, que promoveriam a divisão celular. A interação também não foi benéfica nas culturas de B. cheilantha (Gutiérrez et al, 2011b); já, para Cecropia glaziovii Snethl., a interação permitiu obter uma média de 15 brotações por segmento nodal (Alves, 2010). Siwach & Gill (2011) reportaram um número de brotos duas vezes maior aos 60 dias da multiplicação in vitro de Ficus religiosa L., em vez de aos 21 dias, comportamento diferente do observado para T. guianensis, para a qual, aos 60 dias, independentemente do explante utilizado, o maior número médio de brotos (3,21) observado (Figura 2C) assemelha-se ao encontrado aos 30 dias de inoculação.…”