Determinação da variabilidade em isolados de Colletotrichum lindemuthianum por meio de marcadores morfológicos e culturais

Souza, Brígida; Souza, Elaine Aparecida de; Mendes-Costa, Maria Cristina

doi:10.1590/s1413-70542007000400009

Cited by 13 publications

(12 citation statements)

References 3 publications

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“…isolates into the groups formed in the tree was mainly due to the colony diameter characteristic. Souza et al (2007) identified the shape of Colletotrichum lindemuthianum conidia as a morphological marker that could be used for genetic analyses; Alves et al (2008) studied a collection of isolates, identified as Lasiodiplodia theobromae, and potential cryptic species based on differences in conidial morphology and on sequence data from the ITS regions and EF1-α gene; and Jeewon et al (2004) reported that, for Pestalotiopsis species, the pigmentation of median cells and morphology of apical appendages are the most import characters to distinguish groups. These reports corroborate the studies of Wei & Xu (2004) and Liu et al (2007), who used morphological characters as markers for species or isolate differentiation.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil

Harakawa

Figueredo

2014

Pesq. agropec. bras.

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-The objective of this work was to characterize and cluster isolates of Pestalotiopsis species and to identify those that are pathogenic to pecan, based on morphological and molecular characters. Pestalotiopsis spp. isolates were identified by sequencing the internal transcribed spacer (ITS) and β-tubulin regions. Identification methods were compared to indicate the key morphological characters for species characterization. Thirteen isolates were used for the pathogenicity tests. Morphological characterization was performed using the following variables: mycelial growth rate, sporulation, colony pigmentation, and conidial length and width. Ten pathogenic isolates were identified, three as Pestalotiopsis clavispora and three as P. cocculi. The other isolates remained as an undefined species. The morphological characters were efficient for an initial separation of the isolates, which were grouped according to differences at species level, mainly colony diameter, which was identified as an important morphological describer. Beta-tubulin gene sequencing was less informative than the ITS region sequencing for species identification.Index terms: Carya illinoinensis, β-tubulin, ITS rDNA, morphological characters, phylogeny, UPGMA. Identificação e caracterização de espécies de Pestalotiopsis patogênicas à nogueira-pecã no BrasilResumo -O objetivo deste trabalho foi caracterizar e agrupar isolados de espécies de Pestalotiopsis e identificar aquelas patogênicas à nogueira-pecã, com base em caracteres morfológicos e moleculares. Os isolados de Pestalotiopsis spp. foram identificados por meio do sequenciamento das regiões internal transcribed spacer (ITS) e β-tubulina. Os métodos de identificação foram comparados, para indicar os caracteres morfológicos chave para a caracterização das espécies. Treze isolados foram utilizados para os testes de patogenicidade. A caracterização morfológica foi realizada com a utilização das seguintes variáveis: taxa de crescimento micelial, esporulação, pigmentação das colônias, comprimento e largura de conídios. Dez isolados patogênicos foram identificados, três como Pestalotiopsis clavispora e três como P. cocculi. Os outros isolados permaneceram como espécie não definida. Os caracteres morfológicos foram eficientes na separação inicial dos isolados, os quais foram agrupados por diferenças quanto à espécie, principalmente o diâmetro das colônias, identificado como um importante descritor morfológico. O sequenciamento do gene da β-tubulina foi menos informativo do que o sequenciamento da região ITS para a identificação de espécies.

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Section: Resultsmentioning

confidence: 99%

Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil

Harakawa

Figueredo

2014

Pesq. agropec. bras.

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“…The Colletotrichum genus exhibits wide genetic variability and, in addition to sexual recombination, other mechanisms such as the parasexual cycle exist that allow the transfer of genetic material (Castro-Prado et al, 2007) and asexual recombination through conidial anastomosis tubes (CATs) (Roca et al, 2003;Ishikawa et al, 2012). High variability has been observed in morphological and physiological traits, and several of these are important for pathogen development and survival, such as mycelial growth rate, colony diameter, sporulation capacity, and percent germination (Souza et al, 2007). Another important trait used in taxonomy of the genus Colletotrichum is the cytology of the conidia and the presence of septa in the germinated conidia, the latter of which is an important morphological marker (Souza et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…High variability has been observed in morphological and physiological traits, and several of these are important for pathogen development and survival, such as mycelial growth rate, colony diameter, sporulation capacity, and percent germination (Souza et al, 2007). Another important trait used in taxonomy of the genus Colletotrichum is the cytology of the conidia and the presence of septa in the germinated conidia, the latter of which is an important morphological marker (Souza et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Variability of Colletotrichum spp in common bean

Mota¹,

Barcelos²,

Dias³

et al. 2016

Genet. Mol. Res.

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The Colletotrichum genus presents large genetic variability, as demonstrated by the occurrence of several pathogenic races and phenotypic traits. The objective of this study was to characterize 22 strains of C. lindemuthianum and Colletotrichum spp recovered from anthracnose lesions and bean scab, and to verify the relationship between species of the Colletotrichum genus, which inhabit anthracnose and scab lesions. Colony morphology, conidium size, the presence of septa, germination, sporulation, and mycelium growth rates, were analyzed in addition to the presence of mating-type genes, IRAP markers, and pathogenicity. Strains of Colletotrichum spp presented wide variation for all evaluated traits, indicating the presence of different species. Pathogenicity tests verified that the severity of the disease caused by strains of Colletotrichum spp must be evaluated 17 days after inoculation. Molecular analysis showed that only the C. lindemuthianum strains were grouped by the IRAP markers. For the physiological traits, we observed that C. lindemuthianum mycelium growth is slower than that of Colletotrichum spp strains. The information generated in this study confirms variability in the evaluated species of Colletotrichum and may direct future basic and applied studies aiming to control these diseases in common bean.

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“…Cinco dias após a aplicação do patótipo virulento (Sicard et al,1997;Souza et al, 2007), e a indução da resistência das plantas é uma forma de garantir que a resistência não seja quebrada por um período maior de tempo. Também foi observada interação dos tratamentos na redução da severidade da doença.…”

Section: Três Dias Após a Inoculaçãounclassified

Indução de resistência sistêmica à antracnose em feijoeiro-comum pela raça delta avirulenta de Colletotrichum lindemuthianum

Campos

Hampe

Ferreira

et al. 2009

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o potencial da raça delta avirulenta do fungo Colletotrichum lindemuthianum, como protetora contra raças virulentas deste fungo e quanto à capacidade de induzir resistência sistêmica em feijoeiro-comum (Phaseolus vulgaris). Quatro cultivares de feijoeiro foram avaliadas quanto às alterações nas atividades de beta-1,3-glucanase e quitinase, em dois estádios de desenvolvimento (V2 e R6), três dias após a aplicação de suspensão de esporos de C. lindemuthianum raça delta avirulenta, em comparação com aplicações de água e ácido salicílico. As plantas foram, então, infectadas com o patótipo virulento 33/95 de C. lindemuthianum em suspensão e, depois de cinco dias, foram reavaliadas quanto à atividade das enzimas. Observaram-se acréscimos signifi cativos nas atividades da beta-1,3-glucanase e quitinase, após inoculação do fungo indutivo, nas duas avaliações, nos dois estádios de desenvolvimento. As atividades da beta-1,3-glucanase e da quitinase variaram entre as cultivares e entre os estádios de desenvolvimento das plantas. A correlação entre o índice de severidade da doença e a atividade das enzimas foi altamente signifi cativa. O uso de C. lindemuthianum raça delta avirulenta diminuiu a severidade da doença e pode ter potencial para controlar a antracnose do feijoeiro.Termos para indexação: Phaseolus vulgaris, ácido salicílico, beta-1,3-glucanase, controle biológico, enzimas hidrolíticas, quitinase. Induction of systemic resistance to anthracnose in common bean by the avirulent delta race of Colletotrichum lindemuthianumAbstract -The objectives of this work were to evaluate the potential of the avirulent delta race of Colletotrichum lindemuthianum as a protector against virulent races of this fungus and induce systemic resistance to anthracnose in common bean (Phaseolus vulgaris). Four common bean cultivars were evaluated for changes in the activities of beta-1,3-glucanase and chitinase at two common bean developmental stages, V2 and R6, three days after the infection with delta race of C. lindemuthianum, in comparison with control applications of water and salicylic acid. The plants were then infected with a spore suspension of 33/95, a virulent C. lindemuthianum patotype and reevaluated fi ve days later for the enzyme activities. Signifi cant increases in the activities of both beta-1,3-glucanase and chitinase were observed after inoculation of inductive fungus at the two evaluation periods, at both plant development stages. The activities of both beta-1,3-glucanase and chitinase varied among cultivars and among plant development stages. A signifi cant correlation was observed between the disease severity and the enzyme activity. The avirulent delta race of C. lindemuthianum reduced the severity of anthracnose on common bean and may have the potential to control the disease.

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Determinação da variabilidade em isolados de Colletotrichum lindemuthianum por meio de marcadores morfológicos e culturais

Cited by 13 publications

References 3 publications

Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil

Identification and characterization of pathogenic Pestalotiopsis species to pecan tree in Brazil

Variability of Colletotrichum spp in common bean

Indução de resistência sistêmica à antracnose em feijoeiro-comum pela raça delta avirulenta de Colletotrichum lindemuthianum

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